We narrowed to 7,396 results for: tet on
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Plasmid#217706PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRL1 (ADGRL1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-838Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB085d
Plasmid#79209PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-F3
ExpressionBacterialPromoterPlacZ-opt (OR1); PtetAvailable SinceOct. 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAB085c
Plasmid#79208PurposeDirected Evolution of Protein-Protein InteractionsDepositorInsertPlacZ-opt (OR1) luxAB; Ptet 434cI-TnTBR3-FL
ExpressionBacterialPromoterPlacZ-opt (OR1); PtetAvailable SinceNov. 8, 2016AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-pBen-sfGFP
Plasmid#114598Purpose"Output expression of sfGFP under the activation of BenR transcription factor for cell-free expression"DepositorInsertsfGFP
UseSynthetic Biology; Cell-free protein synthesisPromoterPBenAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-BenR
Plasmid#114597Purpose"Strong constitutive expression of transcription factor, BenR, for cell-free expression"DepositorInsertBenR
UseSynthetic Biology; Cell-free protein synthesisPromoterOR2-OR1-PrAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGLTR-FP-GFP
Plasmid#58242PurposeGATEWAY-compatible lentiviral RNAi delivery vector; SFFV-driven eGFP markerDepositorInsertattR1-Cm(R)-CcdB-attR2
UseLentiviral and RNAi; Gateway destionation vectorExpressionMammalianPromoternoAvailable SinceNov. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEN12A-P750
Plasmid#49525PurposeAmpicillin resistant; entry clone containing PtetO-4C5G and attL1 and attL2 sites for recombination into a Gateway destination vector at the attR4 and attR1 sitesDepositorInsertP750
PromoterP750Available SinceJan. 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pAP118
Plasmid#105495PurposepRPF185 derived plasmid encoding an anhydrotetracyclin inducible HupA-SmBiT and HupA-LgBiT fusionsDepositorInsertHupA-SmBiT/HupA-LgBiT
UseAtc-dependent expression in c. difficilePromoterPtetAvailable SinceSept. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG5 CTF-FLAG
Plasmid#217703PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRG5 (ADGRG5 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-226Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF4 CTF-FLAG
Plasmid#217697PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF4 (ADGRF4 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-384Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-STITCH
Plasmid#129535PurposeSTITCH (Synthetic Toplogical Insulator with TetO for CHromatin-engineering) with homology arms for MYC locus insertion.DepositorInsertSTITCH
UseCRISPR; Donor templateAvailable SinceDec. 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFU-cMVIIA-PE
Plasmid#24147DepositorInsertloxP-DsRed-loxP-omega-Conotoxin MVIIA-FLAG-PDGF_R_TM-EGFP (PDGFRB Human, Conus magus, Aequorea victoria)
UseLentiviralExpressionMammalianAvailable SinceMarch 24, 2010AvailabilityAcademic Institutions and Nonprofits only -
pBS-TRE-mTagBFP2-WPRE
Plasmid#193332PurposeExpresses mTagBFP2 under the TRE promoter in mammalian cellsDepositorInsertmTagBFP2
ExpressionMammalianMutationA XhoI site in the coding sequence is silencedPromoterTREAvailable SinceMay 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
pXGFPC-5 Pxyl-dcas9 (Spy)
Plasmid#133316Purposeexpresses dcas9 from Streptococcus pyogenes; repressed with 0.2% glucose, induced with 0.3% xylose; for homologous recombination at the xylose locus in Caulobacter crescentus; tetracycline resistanceDepositorInsertdcas9 (Streptococcus pyogenes)
UseCRISPRPromoterxyloseAvailable SinceFeb. 14, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBEAST-J23101-CocE
Plasmid#114600Purpose"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"DepositorInsertCocE
UseSynthetic Biology; Cell-free protein synthesisPromoterJ23101Available SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pyc128m
Plasmid#158778Purposesoluble GCaMP expressionDepositorInsertGCaMP6m
UseAAVAvailable SinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
p5A0-T
Plasmid#92016PurposeExpresses TALE targeted to lac operator without TEV cut sites, anhydrotetracycline inducible TEV proteaseDepositorInsertsTALE targeting lac operator
Tobacco Etch Virus Protease
UseTALENTags5x Arginine Tag, 6x His Tag, 7x His Tag, and FLAG…MutationS219VPromoterJ23102 Promoter (from Anderson promoter library) …Available SinceJan. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-HA-Ago2
Plasmid#115362PurposeExpression vector to produce HA-tagged human Ago2 protein without N-peptideDepositorAvailable SinceSept. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
Tags6X-His tag followed by thrombin siteExpressionBacterialPromoterT7Available SinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
pKF-D2irTNP2AG-T2APuroR-5kwh
Plasmid#128255PurposeMammalian linearizer gene circuit based on negative feedback in a Flp-In expression system controlling the PuroR gene.DepositorInserthTetR::P2A::EGFP::T2A::PuroR
UseSynthetic Biology; Flp-in expression vectorExpressionMammalianPromoterCMV-D2ir promoterAvailable SinceJan. 2, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-SAP25-126-186(pL91)
Plasmid#124387PurposeE.Coli produced SAP25 SID purified by FPLC nickel columnDepositorAvailable SinceMay 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBT267_(pCA-ATG-intron-tTA2-TRE-tdT3Myc)
Plasmid#36879DepositorInsertstTA2
tdT-3Myc
Tags3 Myc tagsExpressionMammalianMutationinsertion of a beta-globin intron with a loxP sit…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available SinceAug. 8, 2012AvailabilityAcademic Institutions and Nonprofits only -
pHL 651
Plasmid#53016PurposeLacIq + PCon::RBS (st3) tetR::T1 terminator + p15a + CamR. Backbone from pZA32MCS1 (Lutz and Bujard, 1997).DepositorInsertsLacIq
tetR
UseSynthetic BiologyTagsRBS(st3) and T1 terminatorExpressionBacterialPromoterpConAvailable SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1277
Plasmid#53017PurposeAmpR + PLtetO-1::RBS (st7) gfp::T1 terminator + PLlacO-1::RBS (st7) mCherry::Asp terminator + ColE1DepositorInsertsGFP
mCherry
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHL 1278
Plasmid#53018PurposeAmpR + PLtetO-1::RBS (st7) mCherry::T1 terminator + PLlacO-1::RBS (st7) gfp::Asp terminator + ColE1DepositorInsertsmCherry
GFP
UseSynthetic BiologyTagsRBS(st7)ExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceMay 14, 2014AvailabilityAcademic Institutions and Nonprofits only -
pBEST-HipO
Plasmid#114599PurposeStrong constitutive expression of metabolic enzyme, HipO, for cell-free expressionDepositorInsertHipO
UseSynthetic Biology; Cell-free protein synthesisPromoterOR2-OR1-PrAvailable SinceApril 16, 2019AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ratFDH
Plasmid#135813PurposeExpresses rat FDH (also referred to as AldH1L1) in mammalian cells.DepositorAvailable SinceJan. 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual PNG-FLAG PLU-HIS
Plasmid#113173PurposeBaculovirus expression of PNG and PLUDepositorInsertPNG, PLU
UseBaculovirusAvailable SinceAug. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHL718
Plasmid#62826PurposeLim Lab plasmid: AmpR + pLlacO-1::ompC::gfp::T1T2 terminator + PLtetO-1::ipex::mCherry::T1 terminator + ColE1DepositorInsertsgfp
mCherry
UseSynthetic BiologyTagsipex leader and ompC leaderExpressionBacterialPromoterpLlacO-1 and pLtetO-1Available SinceAug. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1855
Plasmid#53041PurposePLlacO-1:glgC::gfp::LVA, PconNoHind:RBS(st3):tetR, PconNoHind:RBS(st3):csrADepositorInsertsGFP
tetR
csrA
UseSynthetic BiologyTagsLVA, RBS(st3), and glgC leaderExpressionBacterialPromoterpConNoHind and pLlac0-1Available SinceMay 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
DoS2
Plasmid#244467PurposeContaining RP4 oriT, RP4 inc and I-SceI enzyme sequence, for eliminating RP4 plasmid and self-cutting.DepositorAvailable SinceNov. 4, 2025AvailabilityAcademic Institutions and Nonprofits only -
pBA1729
Plasmid#246063PurposeBacterial expression of Panoptes locus with 300bp of context upstream and 150bp downstream of the ECOR31 operon cloned under the pTet promoter; p15a oriDepositorInsertPanoptes locus
ExpressionBacterialPromoterpTet promoterAvailable SinceOct. 27, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCK320
Plasmid#242906PurposeaTc-inducible expression of dCas9-AsiADepositorAvailable SinceSept. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
Reckleen_1_Cas9
Plasmid#233456PurposeLevel 2 RECKLEEN plasmid, genome editing of Klebsiella pneumoniaeDepositorInsertLevel 2 RECKLEEN plasmid
UseCRISPRAvailable SinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2C
Plasmid#236535PurposeFor expression of NM2C (dog) in mammalian cellsDepositorInsertMYH14
TagsHAExpressionMammalianMutationWTAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
HA-cNM2B-Flag
Plasmid#236534PurposeFor expression of NM2B (dog) - with HA and FLAG tags in mammalian cellsDepositorInsertMYH10
TagsFLAG and HAExpressionMammalianMutationWTAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMaSat-miRFP670 pc3944
Plasmid#201695PurposeExpresses of miRFP-tagged MaSat in mammalian cellsDepositorInsertMaSat Synthetic construct zinc finger
TagsmiRFP670ExpressionMammalianPromoterCMVAvailable SinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW369 CMV-TO-ZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF(FLP-IN)
Plasmid#236145PurposePlasmid encoding the ZNF35/ZNF250 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertZNF35(5-8)-ZNF250(1-4)-deImmunLink-NZF
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW490 CMV-TO-UTRN-14ZF-realdeImmLink-NZF (FLP-IN)
Plasmid#236149PurposePlasmid encoding the UTRN-14 zinc finger array attached by a deimmunized linker to the NZF transcriptional activation domain, under control of CMV promoter with two TetR binding sitesDepositorInsertUTRN-14ZF-deImmLink-NZF
ExpressionMammalianPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
EW392 CMV-TO-NZF-(GGS)x8-FRB(T2098L) (FLP-IN)
Plasmid#236157PurposeExpresses NZF transcriptional activation domain attached by a GS linker to the mTOR FRB rapamycin binding domain with the T098L mutation, under control of CMV promoter with two TetR binding sitesDepositorInsertNZF-FRB
ExpressionMammalianMutationT2098L in FRBPromoterCMV promoter with two TetR binding sitesAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1821
Plasmid#228327PurposeConstitutive mutant rTetTA expressionDepositorInsertrtetTA mutant
UseSynthetic BiologyExpressionYeastMutationK8NPromoterScPGK1 promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMT1375
Plasmid#228314PurposeConstitutive expression of mutant rTetTADepositorInsertrtetTA mutant
UseSynthetic BiologyTagsThree copies of VP16 (VP48)ExpressionYeastMutationK8NPromoterKpGAPDH promoterAvailable SinceMarch 28, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_SV40-BFP
Plasmid#224861PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from SV40 promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-2O-crScaffold_EF1a-BFP
Plasmid#224862PurposecrRNA scaffold for RfxCas13d expressed from hU6-2xTetO promoter and reporter BFP protein expressed from EF1a promoterDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAMC042z21
Plasmid#209747PurposeRepA(N99D) SC101 T0-BS7 proD SD8 sfGFP( V93AGGA Y151UAGA) ProK 5 tRNA operon tetA-orfL blaDepositorInsertsfGFP - tRNA operon
ExpressionBacterialMutationsfGFP - Valine 93 AGGA quadruplet codon, Tyrosine…Available SinceOct. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRG7 CTF-FLAG
Plasmid#217705PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGR7 (ADGRG7 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-415Available SinceAug. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pToxEXPr6
Plasmid#218290PurposeIntroduction of a cyanamide-inducible I-SceI expression cassette and a TetR-TUP1 expression cassette, use together with pJQR3DepositorInserthph>tCYC1-ISceI.site-Repeat2-pDDI2>ISceI>tSynth3-pHAC1>TetR>TUP1>tABF1-HO(182,1979)
ExpressionYeastAvailable SinceMay 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF3 CTF-FLAG
Plasmid#217696PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF3 (ADGRF3 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-752Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRF1 CTF-FLAG
Plasmid#217694PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRF1 (ADGRF1 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-566Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
HA-MBP-TEVpCS-ADGRD2 CTF-FLAG
Plasmid#217688PurposeMammalian expression of Adhesion G Protein-Coupled Receptor transmembrane domainsDepositorInsertADGRD2 (ADGRD2 Human)
TagsFLAG and HAExpressionMammalianMutationDeletion of residues 1-636Available SinceApril 18, 2024AvailabilityAcademic Institutions and Nonprofits only