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Hygro-Bem1-mCherry-AID

Plasmid

#173925

Purpose

Plasmid designed for the genomic tagging of saccharomyces cerevisiae Bem1 with mCherry-AID

Depositor

Liedwij Laan

Article

Daalman et al Philos Trans R Soc Lond B Biol Sci. 2023

Inserts

Partial Bem1 C-terminus
hygromycin marker
Bem1 downstream sequence

Tags

mCherry- AID

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_mcherry

Plasmid

#167894

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Sept. 13, 2022

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_mcherry

Plasmid

#167870

Purpose

PiggyBac compatible plasmid expressing dCas9

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only
pET3a-FruK

Plasmid

#186256

Purpose

Overexpression of FruK for purification

Depositor

Liskin Swint-Kruse, Emile Van Schaftingen

Article

Veiga-da-Cunha et al Protein Expr Purif. 2000 Jun;19(1):48-52

Insert

1-phosphofructokinase (fruK E. coli)

Expression

Bacterial

Promoter

T7

Available Since

July 13, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Hygro_zhang2.0

Plasmid

#167915

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA EYFP_zhang2.0

Plasmid

#167914

Purpose

Lentiviral vector for expressing U6 MS2-sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_KanR neo

Plasmid

#167893

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_iRFP713

Plasmid

#167892

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Hygro

Plasmid

#167891

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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NAX_CAGGS_CAS9_VPR_Blasto

Plasmid

#167888

Purpose

PiggyBac compatible plasmid expressing Cas9-VPR

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

Cas9-VPR

Use

CRISPR

Available Since

Feb. 9, 2022

Availability

Academic Institutions and Nonprofits only

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