We narrowed to 5,913 results for: pcDNA 3.1

1. NC3 pCDNA3.1 FLAG WTX (1-804)

   Plasmid

   #36958

   Depositor

   Randall Moon

   Article

   Major et al Science. 2007 May 18;316(5827):1043-6.

   Insert

   WTX (AMER1 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 24, 2012

   Availability

   Academic Institutions and Nonprofits only

2. pcDNA3.1-2xFLAG-2xSTREP-CCND1(T286A)

   Plasmid

   #172647

   Purpose

   Expresses 2xFLAG-2xSTREP-tagged cyclin D1(T286A) in mammalian cells

   Depositor

   Michele Pagano

   Article

   Simoneschi et al Nature. 2021 Apr;592(7856):789-793. doi:

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Tags

   2xFLAG-2xSTREP

   Expression

   Mammalian

   Mutation

   Thr286Ala

   Promoter

   CMV

   Available since

   Aug. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNATM3.1/V5-His TOPO-PKCi-A120E

   Plasmid

   #55687

   Purpose

   expresses constitutively active human PKCi tagged with V5

   Depositor

   Pedro Salas

   Article

   Wald et al J Cell Sci. 2008 Mar 1;121(Pt 5):644-54.

   Insert

   Homo sapiens protein kinase C, iota (PRKCI) (PRKCI Human)
   

   Use

   Tags

   V5/His

   Expression

   Mammalian

   Mutation

   A120E

   Promoter

   CMV

   Available since

   July 28, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA3.1(+)/AUG-nLuc-3XFLAG-PEST

   Plasmid

   #127312

   Purpose

   Express 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon

   Depositor

   Jeremy Wilusz

   Article

   Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

   Insert

   nanoLuciferase
   

   Use

   Tags

   3xFLAG and PEST

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. (137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)

   Plasmid

   #160740

   Purpose

   Expresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobody

   Depositor

   Christina Woo

   Article

   Ramirez et al ACS Chem Biol. 2020 Apr 17;15(4):1059-10

   Insert

   HA-nEPEA-(EAAAK)4-OGT(4)
   

   Use

   Tags

   HA-Tag

   Expression

   Mammalian

   Mutation

   Promoter

   T7/CMV

   Available since

   May 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3.1(+) Laccase2 MCS-ciRS7 Exon

   Plasmid

   #69900

   Purpose

   Expresses the human ciRS7/CDR1as circular RNA in mammalian cells

   Depositor

   Jeremy Wilusz

   Article

   Kramer et al Genes Dev. 2015 Oct 8.

   Insert

   ciRS7 (stw, CDR1-AS Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA3.1 LIC 6A CARD8-FLAG S297A

   Plasmid

   #169949

   Purpose

   Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

   Depositor

   Hao Wu

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S297A

   Promoter

   CMV

   Available since

   May 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3.1-dCas9-MQ1(C141S)-EGFP

   Plasmid

   #89635

   Purpose

   Targeted CpG methylation

   Depositor

   Margaret Goodell

   Article

   Lei et al Nat Commun. 2017 Jul 11;8:16026. doi: 10

   Insert

   site-specific DNA-methyltransferase SssI
   

   Use

   CRISPR

   Tags

   3*FLAG, 6*His, and T2A-EGFP

   Expression

   Mammalian

   Mutation

   C141S, TGC-->TCC

   Promoter

   CMV

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-Flag HBx (G124L, I127A)

   Plasmid

   #42598

   Depositor

   Ding Xue

   Article

   Geng et al Proc Natl Acad Sci U S A. 2012 Nov 6;109

   Insert

   Hepatitis B virus protein X (G124L, I127A)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   G124L I127A

   Promoter

   Available since

   March 22, 2013

   Availability

   Academic Institutions and Nonprofits only

10. pcDNA3.1-tvD-Cit-tevs-DHFR

    Plasmid

    #116054

    Purpose

    Reporter plasmid for measuring protease activity

    Depositor

    Michael Elowitz

    Article

    Gao et al Science. 2018 Sep 21;361(6408):1252-1258

    Insert

    tvD-Cit-tevs-DHFR
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1(+)-Myr-DnaJB1-PKAcat-mCherry

    Plasmid

    #181853

    Purpose

    mCherry-tagged DnaJB1-PKA catalytic subunit fusion with N-terminal myristoylation site added; for mammalian expression.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    Myr-DnaJB1-PKAcat-mCherry (PRKACA, DNAJB1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+) ZKSCAN1 MCS-HIPK3 Exon

    Plasmid

    #69905

    Purpose

    Expresses the human HIPK3 exon 2 circular RNA in mammalian cells

    Depositor

    Jeremy Wilusz

    Article

    Kramer et al Genes Dev. 2015 Oct 8.

    Insert

    HIPK3 (HIPK3 Fly, Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Expresses aa1-194 of HIPK3

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1(-) Full length Her2(ERBB2)-Fc

    Plasmid

    #194330

    Purpose

    Mammalian expression of full length Human epidermal growth factor (Her)-2-Fc fusion protein

    Depositor

    Alexander McLellan

    Insert

    Her2-Fc (ERBB2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pcDNA3.1/CIP2A(1-905) R522D V5 His

    Plasmid

    #119289

    Purpose

    CIP2A R522D V5 overexpression, CIP2A dimer defective mutant

    Depositor

    Jukka Westermarck

    Article

    Wang et al EMBO Rep. 2017 Mar;18(3):437-450. doi: 1

    Insert

    KIAA1524 (CIP2A Human)
    

    Use

    Tags

    V5 His

    Expression

    Mammalian

    Mutation

    R522D

    Promoter

    T7

    Available since

    Jan. 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1 SARS-CoV-2 S D614-trunc

    Plasmid

    #158076

    Purpose

    Expresses SARS-CoV-2 spike protein with a truncation

    Depositor

    Jeremy Luban

    Article

    Yurkovetskiy et al Cell. 2020 Sep 15. pii: S0092-8674(20)31

    Insert

    SARS-CoV-2 spike (S )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1 GIL-11 myc TEV TwinStrep

    Plasmid

    #199627

    Purpose

    Eucaryotic expression plasmid for chimeric cytokine of IL-11 and LIF (GIL-11) with a TwinStrep-tag

    Depositor

    Jurgen Scheller

    Article

    Rafii et al Commun Biol. 2023 Apr 15;6(1):418. doi:

    Insert

    GIL-11
    

    Use

    Tags

    TwinStrep-tag

    Expression

    Mammalian

    Mutation

    Promoter

    T7

    Available since

    May 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA 3.1 (+) FLAG-Ago2 S387E

    Plasmid

    #60653

    Purpose

    Expresses CMV-FLAG-Ago2 (N-term tag) S387E mutant in mammalian cells

    Depositor

    Carl Novina

    Article

    Horman et al Mol Cell. 2013 May 9;50(3):356-67. doi:

    Insert

    Argonaute 2 (AGO2 Human)
    

    Use

    Tags

    CMV-FLAG-Ago2 (N-term tag)

    Expression

    Mammalian

    Mutation

    S387E mutant

    Promoter

    CMV

    Available since

    Dec. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-CMV-CIBN-mCherry-VAMP2

    Plasmid

    #178573

    Purpose

    Mammalian expression plasmid of CIBN-VAMP2 of Opto-vTrap

    Depositor

    Won Do Heo

    Article

    Won et al Neuron. 2022 Feb 2;110(3):423-435.e4. do

    Insert

    CIBN-mCherry-VAMP2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3.1 FLIPglu-30uDelta13V ECFP-W67G

    Plasmid

    #18017

    Depositor

    Wolf Frommer

    Article

    Takanaga et al FASEB J. 2010 Aug . 24(8):2849-58.

    Insert

    FLIPglu D154A Delta13V ECFP-W67G
    

    Use

    Tags

    ECFP-W67G and Venus

    Expression

    Mammalian

    Mutation

    mglB-D154A; non-functional ECFP

    Promoter

    Available since

    Feb. 17, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1/Myc-His(-)-HSulf-2 deltaCC

    Plasmid

    #13006

    Depositor

    Steven Rosen

    Article

    Morimoto-Tomita et al J Biol Chem. 2002 Dec 20. 277(51):49175-

    Insert

    human sulfatase 2 deltaCC (SULF2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    changed Cysteine 88 to Alanine

    Promoter

    Available since

    Oct. 30, 2006

    Availability

    Academic Institutions and Nonprofits only