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Plasmid#68611PurposeGateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plantsDepositorTypeEmpty backboneUseTagsModified EAR motif plant-specific repression doma…ExpressionPlantMutationPromoterAvailable sinceJune 24, 2016AvailabilityAcademic Institutions and Nonprofits only
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H1.99.luc
Plasmid#193663PurposeExpresses a truncated Polymerase III H1 promoter of 99 nucleotides (H1-99)DepositorInsertTruncated Pol III H1 promoter (H1.99) + 41 nucleotides
UseLuciferaseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
Linked mRuby3 VLP
Plasmid#182430PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter) and fusion protein VP2C-GFP-mRuby3 (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP-mRuby3
deltaVP1
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1 and GAL10Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
Coexpressed mRuby3 VLP
Plasmid#182431PurposeYeast integrative plasmid for expressing Murine polyomavirus deltaVP1 (GAL1 promoter), VP2C-GFP (GAL10 promoter), and VP2C-mRuby3 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).DepositorInsertsVP2C-GFP
deltaVP1
VP2C-mRuby3
UseSynthetic BiologyTagsExpressionYeastMutationPromoterGAL1, GAL10, and GAL7Available sinceDec. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
PRE-Hsp70BbCas9_1.0
Plasmid#190795PurposePRE-Hsp70Bb-Cas9-T2A-eGFP; heat-shock inducible Cas9-T2A-eGFP expression regulated by upstream Polycomb response elements. Step 1 in cloning processDepositorInsertPRE-Hsp70Bb-Cas9-T2A-eGFP
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET11-PC(75-228)-H6
Plasmid#183779PurposeExpress PC(75-228)-H6 in E. coli. PC(75-228)-H6 was used for GST-CBP pull-down assays.DepositorInsertPolycomb (Pc Fly)
UseTags6 His Tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET-SU(Z)12(624-900)-H6
Plasmid#183772PurposeExpress of SU(Z)12(624-900)-H6 in E. coli. Purified SU(Z)12(624-900)-H6 was used for raising polyclonal anti-SU(Z)12 antibody in guinea pigs.DepositorInsertSU(Z)12 residues 624-900 (Su(z)12 Fly)
UseTagsHis tag at the C-terminusExpressionBacterialMutationPromoterT7Available sinceApril 21, 2022AvailabilityAcademic Institutions and Nonprofits only -
L1-Ck1-hs1-l-chlo
Plasmid#163937PurposeFor integration in the chloroplast trnG_trnfM region: HS1-L, genome position: 40216_ 41843, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha trnG_trnfM region left homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck4-hs1-r-chlo
Plasmid#163940PurposeFor integration in the chloroplast trnG_trnfM region: HS1-R, genome position: 41847_ 43622, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha trnG_trnfM region right homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
L1-Ck4-hs2-r-chlo
Plasmid#163941PurposeFor integration in the chloroplast rbcL_trnR region: HS2-R, genome position: 57681- 59304, Genbank accession no. MH635409DepositorInsertMarchantia polymorpha rbcL_trnR region right homologous sequence
UseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 30, 2021AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET29b-AviTag-mEGFP-dspB(E184Q W330Y)-6xHis
Plasmid#176575PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein mEGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAvitag, Hexahistidine tag, and mEGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceNov. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pV5endoNA2
Plasmid#174729PurposeExpresses V5-endoNA2 fusion protein for detection of polysialic acid. The endoNA2 is a catalytically inactive form of endosialidase from bacteriophage PK1A2.DepositorInsertV5 epitope tag
UseTagsHistagExpressionBacterialMutationPromoterT5Available sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-2
Plasmid#48146PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPK1E-1
Plasmid#48145PurposeShuttle vector E. coli/B.meg., encoding a promoter for RNA polymerase of the phage K1EDepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Ring1bCD
Plasmid#235574PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Ring1b fused with scFV to programme H2AK119ub.DepositorInsertRing1b (Rnf2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Prdm9CD
Plasmid#235577PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Prdm9 fused with scFV to programme H3K4me3DepositorInsertPrdm9 (Prdm9 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Prdm9CD-CatMut
Plasmid#235586PurposeDox-inducible expression of control catalytic-mutant Prdm9 CD fused with scFVDepositorInsertPrdm9 (Prdm9 Mouse)
UseCRISPRTagsExpressionMutationG282APromoterAvailable sinceApril 30, 2025AvailabilityAcademic Institutions and Nonprofits only -
pPB_TRE3G_scFV-sfGFP-Setd2CD
Plasmid#235579PurposeDox-inducible expression of the catalytic-domain (CD) of epigenetic effector Setd2 fused with scFV to programme H3K36me3DepositorInsertSetd2 (Setd2 Mouse)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceApril 29, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNOC-CRISPR-GFP
Plasmid#100009PurposeExpresses Cas9-GFP and sgRNA in Nannochloropsis oceanica, contains hygromycin resistance cassette, for integration into geneomeDepositorTypeEmpty backboneUseAlgae, nannochloropsis expressionTagsCas9-GFPExpressionMutationPromoterRibi promoterAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
pNOC-ARS-CRISPR-v2
Plasmid#99863PurposeExpresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.DepositorTypeEmpty backboneUseAlgae, nannochloropsisTagsExpressionMutationPromoterRibiAvailable sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only