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We narrowed to 14,439 results for: eng

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Showing: 1861 - 1880 of 14439 results

  1. pCIFR-4

    Plasmid
    #227664
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Sm) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Publication
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Expression
    Bacterial
    Promoter
    p14G-BCD2
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227664-plasmid-map-sequence-id-458897

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  2. pCIFR-2

    Plasmid
    #227663
    Purpose
    Integration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette (Km) used for selecting for the integration can be removed in a later stage with pFNC.
    Depositor
    Pablo Ivan Nikel
    Publication
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Insert
    msfGFP
    Expression
    Bacterial
    Promoter
    p14G-BCD2
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227663-plasmid-map-sequence-id-458896

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  3. pFNC-6

    Plasmid
    #227669
    Purpose
    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.
    Depositor
    Pablo Ivan Nikel
    Publication
    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1
    Inserts
    BxbI phage integrase
    sacB
    Expression
    Bacterial
    Promoter
    PEM7
    Available since
    Jan. 22, 2025
    Availability
    Academic Institutions and Nonprofits only
    227669-plasmid-map-sequence-id-458626

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  6. pCHC030 (∆CBBp ∆MBH ∆SH)

    Plasmid
    #226205
    Purpose
    Electroporation knockout plasmid (pK18sB) to delete the CBBp, membrane-bound hydrogenase, soluble hydrogenase, and intervening sequences (∆CBBp ∆MBH ∆SH).
    Depositor
    Christopher Johnson
    Publication
    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10
    Insert
    Homology arms for CBBp, MBH, and SH operons, and intervening sequencessequences.
    Use
    Synthetic Biology
    Mutation
    ∆cbbR ∆cbbLSXYEFPTZGKA ∆hoxKGZMLOQRTV ∆hypA1B1F1C…
    Available since
    Nov. 18, 2024
    Availability
    Industry, Academic Institutions, and Nonprofits
    226205-plasmid-map-sequence-id-453511

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  7. pACB143

    Plasmid
    #222214
    Purpose
    For recombinant expression of vanB (PP_3737 from Pseudomonas putida) with the A24P mutation and an N-terminal thrombin-cleavable His tag
    Depositor
    Gregg Beckham
    Publication
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    vanB(A24P)
    Tags
    N-terminal, thrombin-cleavable 6x His tag
    Expression
    Bacterial
    Mutation
    Changed Alanine 24 to Proline
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222214-plasmid-map-sequence-id-438176

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  8. pACB109

    Plasmid
    #222208
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the K10N mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Publication
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (K10N)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Lys10 to Asn
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222208-plasmid-map-sequence-id-437369

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  9. pACB110

    Plasmid
    #222209
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G258D mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Publication
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA (G258D)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Gly258 to Aspartate
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222209-plasmid-map-sequence-id-437372

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  10. pACB111

    Plasmid
    #222210
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the G76V mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Publication
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(G76V)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed glycine 76 to valine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222210-plasmid-map-sequence-id-437376

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  11. pACB112

    Plasmid
    #222211
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the S11R mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Publication
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(S11R)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Serine 11 to Arginine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222211-plasmid-map-sequence-id-437378

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  12. pACB113

    Plasmid
    #222212
    Purpose
    For recombinant expression of fghA (PP_1617 from Pseudomonas putida) with the W15C mutation and a C-terminal His tag
    Depositor
    Gregg Beckham
    Publication
    Bleem et al Metab Eng. 2024 Jul;84:145-157. doi: 10.
    Insert
    fghA(W15C)
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    Changed Tryptophan 15 to Cysteine
    Promoter
    T7
    Available since
    Aug. 26, 2024
    Availability
    Academic Institutions and Nonprofits only
    222212-plasmid-map-sequence-id-437381

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  13. pTC3cIfm

    Plasmid
    #193778
    Purpose
    Expresses frame-shifted cI under the control of PLTetO-1 promoter, p15A origin of replication, Chloramphenicol selection
    Depositor
    Sangram Bagh
    Publication
    Sarkar et al J Biol Eng. 2019 Mar 1;13:20. doi: 10.11
    Insert
    Frame-shifted CI
    Use
    Synthetic Biology
    Expression
    Bacterial
    Promoter
    PLTetO-1
    Available since
    April 10, 2023
    Availability
    Academic Institutions and Nonprofits only
    193778-plasmid-map-sequence-id-389809

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  14. pTS2333_Tier3(Lenti_inverse)-YPet_Puro

    Plasmid
    #169664
    Purpose
    Tier-3 vector for stable integration via lentiviral transduction in inverted direction with polyA carrying a constitutive expression cassette for puromyine and Ypet (PCMV-5'LTR-Psi-gag-env-PRPBSA-YPet-p2A-PuroR-pA::A2-pA::A3-pA-WPRE-3'LTR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PhCMV / RPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169664-plasmid-map-sequence-id-365639

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  15. pTS2361_Tier3(SB)-rtTA-YPet_PuroR

    Plasmid
    #169657
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via sleeping beauty transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169657-plasmid-map-sequence-id-365632

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  16. pTS1042

    Plasmid
    #169620
    Purpose
    Tier-2 co-expression vector encoding PhCMV-driven SEAP coupled to fluorescent reporter iRFP670 and PmPGK1-driven secreted Nluc coupled to fluorescent reporter mTagBFP2 (PhCMV-SEAP-p2A-iRFP670-pA::PmPGK1-IgkSS-Nluc-p2A-mTagBFP2-pA::A3-pA).
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PCMV-driven expression of SEAP and iRFP and PPGK-driven expression of secreted Nluc and mTagBFP2
    Expression
    Mammalian
    Promoter
    PhCMV / PPGK
    Available since
    June 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    169620-plasmid-map-sequence-id-365622

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  17. pTS2363_Tier3(PB)-rtTA-YPet_PuroR

    Plasmid
    #169659
    Purpose
    Tier-3 vector for stable stable integration of up to three cassettes via Piggy Bac transposase including a YPet marker and PuroR resistance gene and a PPGK-driven rtTA transactivator (5'ITR-A1-pA::PPGK-rtTA-pA::PRPBSA-YPet-p2A-PuroR-pA-3'ITR)
    Depositor
    Martin Fussenegger
    Publication
    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10
    Insert
    PPGK-driven rtTA expression and PRPBSA-driven YPet and PuroR expression
    Expression
    Mammalian
    Promoter
    PRPBSA
    Available since
    June 16, 2022
    Availability
    Academic Institutions and Nonprofits only
    169659-plasmid-map-sequence-id-364557

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Showing: 1861 - 1880 of 14439 results