We narrowed to 4,045 results for: golden gate
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Plasmid#217609PurposeDestination vector with ampicillin resistance and pBR322 origin of replication. Carries E-H CIDAR MoClo overhangs for level 0 or 2 construct cloning.DepositorTypeEmpty backboneUseSynthetic BiologyMutationNoneAvailable SinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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pBH502
Plasmid#215781PurposeNBU2 backbone with LacI(YQR) and P(O1)_sgRNA targeting NanolucDepositorInsertsLacI(YQR)
sgRNA-ribozyme cassette
Nanoluc
UseSynthetic BiologyExpressionBacterialPromoterBt1311, P(O1), and cfxAAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Empty-cleavableELP
Plasmid#219401PurposeInsert protease recognition site with BsaI to make custom protease-responsive elastin-like polypeptide (ELP)DepositorInsertEmpty-cleavableELP
Tags6xHisExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-FXa-cleavableELP
Plasmid#219403PurposeFactor Xa-responsive elastin-like polypeptide (ELP)DepositorInsertFXa-cleavableELP
Tags6xHisExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-EK-cleavableELP
Plasmid#219404PurposeEnterokinase-responsive elastin-like polypeptide (ELP)DepositorInsertEK-cleavableELP
Tags6xHisExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET22b-Thr-cleavableELP
Plasmid#219405PurposeThrombin-responsive elastin-like polypeptide (ELP)DepositorInsertThr-cleavableELP
Tags6xHisExpressionBacterialAvailable SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBTK1059
Plasmid#209549PurposeBTK Type 5 homology arm for Golden Gate assemblyDepositorInsertDownstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
UseSynthetic BiologyAvailable SinceFeb. 1, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
GGDestX2-Amp
Plasmid#157654PurposeGolden Gate assembly and mammalian expression of dCas9 fusion-proteins plus gRNADepositorTypeEmpty backboneUseSynthetic BiologyExpressionMammalianPromoterU6, CBhAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ131C-RZ-Lb
Plasmid#134347PurposeGolden Gate entry vector; empty vector to clone the 1st LbCas12a crRNA under OsU6 promoterDepositorTypeEmpty backboneUseCRISPRAvailable SinceMarch 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pICSL12022
Plasmid#117510PurposeAtAG promoter Golden Gate Level 0DepositorInsertBsaI:GGAG:pAG:AATG:BsaI
ExpressionPlantPromoterAtAGAvailable SinceFeb. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJet1.2 'rncS
Plasmid#117129Purposesmc gene upstream homology regionDepositorInsert'rncS
UseGolden gate donor vector for gene targeting in ba…Available SinceNov. 14, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGGE003
Plasmid#48854PurposeProvides the At1g04880 terminator as GreenGate module.DepositorInsertAt1g04880 terminator
UseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGC014
Plasmid#48827PurposeProvides GFP as GreenGate coding sequence module.DepositorInsertGFP
UseGolden gate compatible cloning vectorMutationA206KAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pGGC024
Plasmid#48829PurposeProvides BFP as GreenGate coding sequence module.DepositorInsertBFP
UseGolden gate compatible cloning vectorAvailable SinceJan. 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
EC71174
Plasmid#154067PurposeLevel 2 Golden Gate vector, pL2M-HvHSP17-CREU5-UBQ-loxmCHERRYER-eGFPERDepositorInsertsHS Cre recombinase
Ubiquitin promoter driving mCherry with the 35S terminator, flanked by lox sites, followed by eGFP with the Actin terminator
HYGROMYCIN Resistance Cassette
UseCre/Lox and Synthetic Biology; Golden gateExpressionBacterial and PlantMutationThe Arabidopsis thaliana U5 small nuclear ribonuc…Promoter35S promoter, Hordeum vulgare HSP 17 promoter use…Available SinceSept. 29, 2020AvailabilityAcademic Institutions and Nonprofits only -
PB-hEF1A-MG-U6-sgRNA
Plasmid#175505PurposeAll-in-one piggyBac transposon destination vector for hEF1a expression of Mega Gate cloned elements and hU6 expression of Golden Gate cloned guide RNAsDepositorTypeEmpty backboneUseCRISPR and Synthetic BiologyExpressionMammalianPromoterhEF1a, U6Available SinceOct. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCBL101gg
Plasmid#199735PurposeGolden Gate recipient vector for transgene overexpressionDepositorInsertsacB
ExpressionPlantAvailable SinceApril 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pBH504
Plasmid#215782PurposeNBU1 backbone with constitutive dCas9, LacI(YQR), P(O1)_sgRNA targeting B. theta amylopectin SusC geneDepositorInsertsLacI(YQR)
sgRNA-ribozyme cassette
dCas9
UseSynthetic BiologyExpressionBacterialPromoterBt1311, P(O1), and P1Available SinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICSL12015
Plasmid#117506PurposeAtUBI10 promoter Golden Gate Level 0DepositorInsertBsaI:GGAG:pUBI10:AATG:BsaI
ExpressionPlantPromoterAtUBI10Available SinceOct. 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJPC259
Plasmid#234531PurposeCre/Lox 71/66 Recombinase switch; plant T-DNADepositorInsertRUBY
ExpressionPlantMutationnoneAvailable SinceApril 18, 2025AvailabilityAcademic Institutions and Nonprofits only