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pCFJ2560 - pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Plasmid

#159825

Purpose

Single copy or array insertion by MosTI using split unc-119 selection

Depositor

Christian Froekjaer-Jensen

Article

El Mouridi et al G3 (Bethesda). 2022 Jul 28. pii: 6651068

Insert

pEXP(mosTI, unc-119)[Peft-3 | ceGFP(PATC, NLS) | tbb-2 3'UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-208

Plasmid

#135345

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 208 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-208

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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AIDx-ID-468

Plasmid

#135346

Purpose

Expression of intradomain insertion of truncated human cytosine deaminase, hAIDx, at position 468 of nSpyCas9

Depositor

Alex Hewitt

Article

Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

Insert

nSpyCas9-hAIDx-ID-468

Use

CRISPR

Expression

Mammalian

Mutation

WT

Available Since

Oct. 1, 2020

Availability

Academic Institutions and Nonprofits only

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myc-Miro2 (lacking TM)

Plasmid

#127614

Purpose

Human Miro2 with the transmembrane deleted

Depositor

Josef Kittler

Article

Lopez-Domenech et al EMBO J. 2018 Feb 1;37(3):321-336. doi: 1

Insert

Miro2 (RHOT2 Human)

Tags

myc

Expression

Mammalian

Mutation

Transmembrane deletion

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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Met133I, M1381I, M186I ELL2

Plasmid

#127263

Purpose

For in vitro translation of human ELL2 with Met133I, M1381I, M186I

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133ILeu, M1381ILeu, M186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Three Mets (133, 138, 186) to Ileu

Promoter

T7

Available Since

Nov. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA tagged Sph ELL2

Plasmid

#127270

Purpose

For in vitro translation of human WT ELL2 with internal HA tag

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (ELL2 Human)

Use

In vitro translation

Mutation

WT Mets and HA tag at SphI site (8th Met)

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu ELL2

Plasmid

#127260

Purpose

For in vitro translation of human ELL2 with Met138 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met138 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met186ILeu ELL2

Plasmid

#127261

Purpose

For in vitro translation of human ELL2 with Met186 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met186 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met186 to Ileu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met138ILeu, Met186ILeu ELL2

Plasmid

#127265

Purpose

For in vitro translation of human ELL2 with Met138ILeu, Met186ILeu

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met138ILeu, Met186ILeu) (ELL2 Human)

Use

In vitro translation

Mutation

Two Mets (138,186) to ILeu Quikchange

Promoter

T7

Available Since

July 15, 2019

Availability

Academic Institutions and Nonprofits only

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Met133ILeu ELL2

Plasmid

#127259

Purpose

For in vitro translation of human ELL2 with Met133 mutated to Ileu to ask if there is internal initiation

Depositor

Christine Milcarek

Article

Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

Insert

ELL2 (Met133 to Ileu) (ELL2 Human)

Use

In vitro translation

Mutation

Met133 to Ileu Quikchange

Promoter

T7

Available Since

July 12, 2019

Availability

Academic Institutions and Nonprofits only

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