We narrowed to 15,404 results for: nans

1. pET21-pelB-LaG-16-4GS-LaG-2

   Plasmid

   #172764

   Purpose

   Bacterial expression of dimerized anti-GFP nanobodies LaG-16 and LaG-2 (4GS linker)

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-16-4GS-LaG-2
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pre_IV_CS8_7569_v1

   Plasmid

   #126865

   Purpose

   7886 bases long scaffold with Zn-dependent self-cleaving DNAzyme cassettes, from compatibility group 8, made by method IV, yields linear 7569 b long scaffolds after Zn incubation

   Depositor

   Hendrik Dietz

   Article

   Engelhardt et al ACS Nano. 2019 Apr 22. doi: 10.1021/acsn

   Insert

   IV_CS8_7569_v1
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pGLOW31

   Plasmid

   #172698

   Purpose

   C. elegans mScarlet co-injection marker

   Depositor

   Ryan Doonan

   Article

   Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

   Insert

   Pmyo-3::mScarlet
   

   Use

   Tags

   mScarlet

   Expression

   Worm

   Mutation

   Promoter

   myo-3

   Available since

   Aug. 30, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

4. pCS2-mCherry-Rab11a (S25N)

   Plasmid

   #184036

   Purpose

   Expresses dominate negative mCherry Rab11a in mammalian cells and zebrafish embryos

   Depositor

   Heidi Hehnly

   Article

   Krishnan et al Life Sci Alliance. 2022 Mar 18;5(7). pii

   Insert

   Rab11
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Serine 25 changed to Asparagine

   Promoter

   CMV IE94

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNOC-ARS-CRISPR-v2

   Plasmid

   #99863

   Purpose

   Expresses Cas9-Nlux and sgRNA. Compact backbone contains CEN/ARS. Hygromycin selection marker.

   Depositor

   Eva Farre

   Article

   Poliner et al ACS Synth Biol. 2018 Apr 20;7(4):962-968

   Type

   Empty backbone

   Use

   Algae, nannochloropsis

   Tags

   Expression

   Mutation

   Promoter

   Ribi

   Available since

   Feb. 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLyGo-Ec-8

   Plasmid

   #163136

   Purpose

   pLyGo cloning vector for surface display in E. coli of a sequence of interest (LPMO). Vector encoding the LppSP-OmpA surface expression anchor and the LyGo cassette (SapI-ccdB-SapI)

   Depositor

   Morten Norholm

   Article

   Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   P(rhaB)

   Available since

   May 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. B2094_CRY2olig-miRFP

   Plasmid

   #215114

   Purpose

   Expresses CRY2olig (E490G CRY2 1-498) fused to miRFP in mammalian cells

   Depositor

   Chandra Tucker

   Article

   Hernandez-Candia et al Nat Chem Biol. 2024 Jan 8. doi: 10.1038/

   Insert

   CRY2olig-miRFP (CRY2 Mustard Weed)
   

   Use

   Tags

   miRFP

   Expression

   Mammalian

   Mutation

   E490G

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTpPuc3

   Plasmid

   #62864

   Purpose

   Episome vector for Thalassiosira pseudonana (pUC-based)

   Depositor

   Hamilton Smith

   Article

   Karas et al Nat Commun. 2015 Apr 21;6:6925. doi: 10.

   Type

   Empty backbone

   Use

   Synthetic Biology; Episomal vector for thalassios…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. GW1-rogRFP2

   Plasmid

   #207876

   Purpose

   Mammalian expression of the red fluorescent rogRFP2 redox sensor

   Depositor

   Mathew Tantama

   Article

   Radhakrishnan et al ACS Chem Neurosci. 2020 Sep 2;11(17):266

   Insert

   rogRFP2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. AF1521-c003

    Plasmid

    #173111

    Purpose

    Expression of recombinant fusion protein in E. coli

    Depositor

    Lari Lehtiö

    Article

    Sowa et al Cell Rep Methods. 2021 Dec 20;1(8):10012

    Insert

    AF_1521 (AF_RS07675 Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126))
    

    Use

    Tags

    Nanoluc

    Expression

    Bacterial

    Mutation

    KH-M1-L192

    Promoter

    Available since

    Dec. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pROF417

    Plasmid

    #155335

    Purpose

    Constitutive expression LFY-VP16-2A-RLuc in plants

    Depositor

    Matias Zurbriggen

    Article

    Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

    Insert

    PCaMV35S-LFY-VP16-NLS-2A-RLuc-Tnos
    

    Use

    Luciferase and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available since

    Oct. 26, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGex-4T-2_GABARAP

    Plasmid

    #73948

    Purpose

    For recombinant expression of human GABARAP in E.coli

    Depositor

    Dieter Willbold

    Article

    Stangler et al J Biomol NMR. 2001 Oct;21(2):183-4.

    Insert

    GABARAP (GABARAP Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

13. pALOD4 non-binding mutant

    Plasmid

    #111027

    Purpose

    Expresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)

    Depositor

    Arun Radhakrishnan

    Article

    Gay et al Biophys J. 2015 Mar 24;108(6):1459-69. d

    Insert

    Domain 4 of Anthrolysin O
    

    Use

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: S404C, C472A, G501A, T502A, T503A, L5…

    Promoter

    T7

    Available since

    June 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. CapChR1_pmCherry-N1

    Plasmid

    #188031

    Purpose

    Expresses CapChR1-mCherry in mammalian cells

    Depositor

    Peter Hegemann

    Article

    Fernandez Lahore et al Nat Commun. 2022 Dec 21;13(1):7844. doi:

    Insert

    CapChR1-mCherry
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    S63D, L132C, T159C and N258E mutations in CrChR2

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. E3020X

    Plasmid

    #21366

    Depositor

    Gregory Germino

    Article

    Qian et al Proc Natl Acad Sci U S A. 2002 Dec 24. 9

    Insert

    autosomal dominant polycystic kidney disease type I (PKD1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Changed E3020 to stop codon

    Promoter

    Available since

    Feb. 17, 2010

    Availability

    Academic Institutions and Nonprofits only

16. pGLOW77

    Plasmid

    #177338

    Purpose

    C. elegans mNeonGreen co-injection marker

    Depositor

    Ryan Doonan

    Article

    Witten et al MicroPubl Biol. 2023 Jun 14;2023:10.1791

    Insert

    Pmyo-2::mNeonGreen
    

    Use

    Tags

    mNeonGreen

    Expression

    Worm

    Mutation

    Promoter

    myo-2

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET21-pelB-LaM-3

    Plasmid

    #172769

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-3, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-3
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLyGo-Kp-1

    Plasmid

    #163143

    Purpose

    pLyGo cloning vector for a sequence of interest (LPMO) in K. phaffii. Vector encoding the alfa-MF pre-sequence and the LyGo cassette (SapI-ccdB-SapI)

    Depositor

    Morten Norholm

    Article

    Hernandez-Rollan et al ACS Synth Biol. 2021 Apr 16;10(4):897-90

    Type

    Empty backbone

    Use

    Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    AOX1

    Available since

    Aug. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GOMO-GFP

    Plasmid

    #60975

    Purpose

    Rapid selection of recombinant marker-free fluorescent rodent malaria parasites

    Depositor

    Olivier Silvie

    Article

    Manzoni et al Sci Rep. 2014 Apr 23;4:4760. doi: 10.103

    Inserts

    GFP
    mCherry
    hDHFR-yFCU
    

    Use

    Plasmodium gene targeting

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. (SIM)5

    Plasmid

    #126947

    Purpose

    Bacterial expression plasmid for (SIM)5 client to assess SUMO-SIM scaffolds.

    Depositor

    Michael Rosen

    Article

    Banani et al Cell. 2016 Jul 28;166(3):651-663. doi: 1

    Insert

    5 repeats of SUMO interacting motif (SIM) from PIASx, each separated by (GGS)4 (PIAS2 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    unknown

    Available since

    Sept. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge