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pME-mir126new

Plasmid

#67712

Purpose

Replacement for 26033 776 pME-mir-126

Depositor

Nathan Lawson

Article

Nicoli et al Nature. 2010 Apr 22. 464(7292):1196-200.

Insert

mir126a (mir126a Zebrafish)

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pBALU4

Plasmid

#69319

Purpose

Encodes a recombineering cassette for tagging a target gene within a genomic clone with a fluorescent protein. Cassette: FRT-galK-FRT in the 2nd intron of mChO (mCherry variant)

Depositor

Oliver Hobert

Article

Tursun et al PLoS One. 2009;4(3):e4625. doi: 10.1371/

Insert

mChOint-FgF

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only
pEM352

Plasmid

#248764

Purpose

160xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-160xTetO

Expression

Bacterial

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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35S-ZFN-pDGB3alpha1

Plasmid

#240729

Purpose

Encodes a ZFN that targets the broken GUS:NPTII reporter.

Depositor

Jose Alonso

Article

Neubauer et al Plant Biotechnol J. 2025 Sep 21. doi: 10

Insert

ZFN

Expression

Plant

Available Since

Feb. 2, 2026

Availability

Academic Institutions and Nonprofits only

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pEM413

Plasmid

#248765

Purpose

10xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-10xTetO

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pEM415

Plasmid

#248767

Purpose

40xTetO Sponge Plasmid with BsaI sites flanking sponge casette for Golden Gate Assembly

Depositor

Carolina Tropini

Article

McCallum et al Cell. 2026 Feb 19;189(4):1245-1261.e21.

Insert

T7Te-rrnBT1-40xTetO

Expression

Bacterial

Available Since

Jan. 9, 2026

Availability

Academic Institutions and Nonprofits only

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pCK303.306

Plasmid

#242926

Purpose

Expression of an MS2 scRNA J306

Depositor

Jesse Zalatan

Article

Kiattisewee et al ACS Synth Biol. 2025 Jul 22. doi: 10.102

Insert

scRNA MS2 J306

Use

CRISPR and Synthetic Biology

Promoter

Bba_J23119

Available Since

Oct. 14, 2025

Availability

Academic Institutions and Nonprofits only

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pREA12

Plasmid

#245770

Purpose

Allows for mCherry::RBD::T2A::rac-2::mGFP entry into Gateway Vectors

Depositor

Salvatore Cherra

Article

Lamb et al Development. 2025 Aug 1:dev.204678. doi:

Insert

mCherry::RBD::T2A::rac-2::mGFP

Use

Unspecified

Tags

mCherry and mGFP

Available Since

Sept. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA26_sgRNA-A0_M13ps

Plasmid

#231413

Purpose

M13 phagemid constitutively expressing sgRNA-A0. sgRNA-A0 is a dummy 20-nt sequence, containing a Golden Gate cloning site (BsaI).

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Insert

sgRNA-A0

Use

Synthetic Biology

Available Since

Feb. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pSEVA47_dCas9-GFP_YES_i2

Plasmid

#231419

Purpose

This YES-gate plasmid expresses dCas9 from a constitutive promoter, GFP from a promoter repressible by sgRNA-Y, and sgRNA-Y repressible by sgRNA-2.

Depositor

Manish Kushwaha

Article

Pujar et al Nucleic Acids Res. 2024 Dec 27:gkae1256.

Inserts

dCas9
GFP
sgRNA-Y

Use

Synthetic Biology

Available Since

Jan. 30, 2025

Availability

Academic Institutions and Nonprofits only

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