We narrowed to 1,094 results for: gibson assembly
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Plasmid#198308PurposeProduces PVCs tagged with HiBiT on the baseplate; used as assembly/loading control for payload loading assaysDepositorInsertPVCpnf_pvc12-HiBiT
ExpressionBacterialAvailable SinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcCAST-sgRNA_entry (CJT83)
Plasmid#181785PurposeExpresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertAcTnsB, AcTnsC, AcTniQ, AcCas12k
ExpressionBacterialPromoterLac and J23119Available SinceFeb. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pShoCAST-sgRNA_entry (BO1)
Plasmid#181786PurposeExpresses ShoCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).DepositorInsertShoTnsB, ShoTnsC, ShoTniQ, ShoCas12k
ExpressionBacterialPromoterLac and J23119Available SinceMarch 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKG1179_pPV1-EFS
Plasmid#239690PurposeBsaI Golden Gate assembly plasmid encoding the EFS promoterDepositorInsertEFS promoter
UseSynthetic BiologyAvailable SinceOct. 17, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNC-Y
Plasmid#238998PurposeNode C carrying sgRNA-1 that can be used for the assembly of pEND-11Y or pEND-ZYDepositorInsertsgRNA-1 downstream of a weak constitutive promoter and a binding site for sgRNA-2
UseSynthetic BiologyExpressionBacterialMutationWTAvailable SinceJuly 22, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW621
Plasmid#229867PurposeEntry vector with cGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR in slot 5 (for SapTrap assembly)DepositorInsertcGAL[cGAL(DBD)-linker-QFAD]-tbb-3'UTR
ExpressionWormAvailable SinceJuly 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG0586_pPV2-mRuby2
Plasmid#239695PurposeBsaI Golden Gate assembly plasmid encoding the mRuby2 CDSDepositorInsertmRuby2
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
pKG2039_pPV1-CAG
Plasmid#239686PurposeBsaI Golden Gate assembly plasmid encoding the CAG promoterDepositorInsertCAG promoter
UseSynthetic BiologyAvailable SinceJuly 10, 2025AvailabilityAcademic Institutions and Nonprofits only -
MZB164
Plasmid#217842Purpose10xHis-SUMO (Smt3) fused, HRV-3C cleavable, Halotag for rapid cloning/DNA assembly of pulldown targets via SwaI restriction site for E. coli based protein expressionDepositorInsertHaloTag
Tags10x His, HaloTag, and SUMO (SMT3)ExpressionBacterialPromoterT7 PromoterAvailable SinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pHW1143
Plasmid#229866PurposeEntry vector with FRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA in slot 4 (for SapTrap assembly)DepositorInsertFRT-SA-ArtificialExon-3xStopCodons-SL2-AAAA
ExpressionWormAvailable SinceFeb. 10, 2025AvailabilityAcademic Institutions and Nonprofits only