We narrowed to 16,111 results for: form

1. pHYDuet93

   Plasmid

   #42303

   Depositor

   Hideo Iwai

   Article

   Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

   Insert

   NpuDnaEdC6
   

   Tags

   H6-GB1

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEBG-TFII-deltaNLS1

   Plasmid

   #22153

   Depositor

   Ananda Roy

   Article

   Cheriyath et al J Biol Chem. 2000 Aug 25. 275(34):26300-

   Insert

   TFII-I (GTF2I Human)
   

   Tags

   GST and His

   Expression

   Mammalian

   Mutation

   deletion of amino acids 297-304

   Available Since

   Nov. 17, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET SUMO-FL-syt7

   Plasmid

   #213630

   Purpose

   Bacterial expression of SUMO-tagged mouse FL-syt7

   Depositor

   Edwin Chapman

   Article

   Courtney et al Nat Commun. 2023 Nov 27;14(1):7761. doi:

   Insert

   FL-syt7 (Syt7 Mouse)
   

   Tags

   SUMO

   Expression

   Bacterial

   Available Since

   March 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHYBAD94

   Plasmid

   #42305

   Depositor

   Hideo Iwai

   Article

   Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

   Insert

   NpuDnaEC6
   

   Tags

   GB1-H6

   Expression

   Bacterial

   Promoter

   araBAD

   Available Since

   Feb. 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET15b/GgVcl 1-258 A50I mutation

   Plasmid

   #46175

   Depositor

   Susan Craig

   Article

   Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

   Insert

   Vcl 1-258 A50I (VCL Chicken)
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   aa 1-258 (VD1) A50I mutation; talin binding defic…

   Promoter

   T7

   Available Since

   July 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pACEC3GH (Hyg)

   Plasmid

   #110084

   Purpose

   Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3C cleavage site - GFP - His10 Tag

   Expression

   Bacterial

   Available Since

   June 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFLAG_attP

   Plasmid

   #110095

   Purpose

   Vector for low-level constitutive expression in mycobacteria from the Tet promoter. Lacks the Tet repressor. Contains attP for chromosomal integration. Lacks the integrase and thus remains stably integrated in the absence of antibiotic selection. Contains a C-terminal 3xFLAG to allow detection of expression levels by Western blotting.

   Depositor

   Markus Seeger

   Article

   Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

   Type

   Empty backbone

   Tags

   3xFLAG

   Expression

   Bacterial

   Available Since

   June 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET26b_Nb.b201

   Plasmid

   #131404

   Purpose

   E. coli periplasmic expression of nanobody Nb.b201 recognizing human serum albumin

   Depositor

   Andrew Kruse

   Article

   McMahon et al Nat Struct Mol Biol. 2018 Mar;25(3):289-

   Insert

   Nanobody b201
   

   Tags

   6xHis-tag and pelB leader

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCHC042 (∆phaCAB)

   Plasmid

   #226208

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator phaCAB operon, to prevent accumulation of PHB.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for phaCAB
   

   Use

   Synthetic Biology

   Available Since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pCHC005 (∆RE)

    Plasmid

    #226199

    Purpose

    Conjugation knockout plasmid (pK18mobsacB) to delete H16_0006 (Type I restriction endonuclease subunit).

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for type I restriction-modification system restriction subunit H16_A0006
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pESUMO-hRORg1

    Plasmid

    #170330

    Purpose

    Expresses RORg isoform 1 with N-terminal HisSUMO tag in bacteria

    Depositor

    Patrick Griffin

    Article

    Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

    Insert

    RORG isoform 1 (RORC Human)
    

    Tags

    HisSUMO

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCHC039 (∆phcA)

    Plasmid

    #226207

    Purpose

    Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

    Depositor

    Christopher Johnson

    Article

    Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

    Insert

    Homology arms for PhcA
    

    Use

    Synthetic Biology

    Available Since

    Nov. 18, 2024

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. GST-beta-arrestin1

    Plasmid

    #36918

    Depositor

    Robert Lefkowitz

    Article

    Nobles et al J Biol Chem. 2007 Jul 20;282(29):21370-8

    Insert

    beta-arrestin1 (Arrb1 Rat)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    June 27, 2012

    Availability

    Academic Institutions and Nonprofits only

14. pACEC3GH (Apr)

    Plasmid

    #110085

    Purpose

    Replicative, acetamide-based expression vector containing a C-terminal 3C cleavage site and a GPF-fusion with a His10-tag. Suitable for high-level expression levels amenable for large scale purification of proteins.

    Depositor

    Markus Seeger

    Article

    Arnold et al Sci Rep. 2018 Jun 22;8(1):9539. doi: 10.

    Type

    Empty backbone

    Tags

    3C cleavage site - GFP - His10 Tag

    Expression

    Bacterial

    Available Since

    June 5, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEGFPC1/GgVcl 1-258 A50I mutation

    Plasmid

    #46271

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-258 (aka VD1) A50I mutation (VCL Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    1-258 (VD1 domain) A50I mutation

    Promoter

    CMV

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEGFPC1/GgVcl 1-851 A50I mutant

    Plasmid

    #46269

    Depositor

    Susan Craig

    Article

    Cohen et al J Biol Chem. 2006 Jun 9;281(23):16006-15

    Insert

    Vcl 1-851 A50I mutation (VCL Chicken)
    

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    1-851 (Vh) head domain, A50I mutation

    Promoter

    CMV

    Available Since

    July 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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17. pMHBAD14

    Plasmid

    #42304

    Depositor

    Hideo Iwai

    Article

    Oeemig et al FEBS Lett. 2009 May 6;583(9):1451-6. doi

    Insert

    NpuDnaE C-intein
    

    Tags

    GB1-H6

    Expression

    Bacterial

    Promoter

    araBAD

    Available Since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. pESUMO-hRORg2

    Plasmid

    #170329

    Purpose

    Expresses RORg isoform 2 with N-terminal HisSUMO tag in bacteria

    Depositor

    Patrick Griffin

    Article

    Strutzenberg et al J Mol Biol. 2021 Sep 20;433(22):167258.

    Insert

    RORG isoform 2 (RORC Human)
    

    Tags

    HisSUMO

    Expression

    Bacterial

    Mutation

    codon optimized

    Promoter

    T7

    Available Since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PtetA-PLacO3O1 150 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225645

    Purpose

    tetA and LacO3O1 promoters in tandem conformation with 150bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    tetR/tetA and LacO3O1 in tandem conformation
    

    Tags

    mCherry

    Expression

    Bacterial

    Promoter

    tetA and LacO3O1 promoters

    Available Since

    Oct. 15, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. PLacO3O1-PtetA 250 bp with pBELO_L6-PT3-T4_CAMR_TAN+

    Plasmid

    #225648

    Purpose

    LacO3O1 and tetA promoters in tandem conformation with 250bp distance between them, expressing mCherry with pBELO_L6-PT3-T4 backbone and chloramphenicol resistance

    Depositor

    Andre Sanches Ribeiro

    Article

    Chauhan et al Biochemistry. 2024 Jul 12. doi: 10.1021/

    Insert

    LacO3O1 and tetR/tetA in tandem conformation
    

    Tags

    mCherry

    Expression

    Bacterial

    Promoter

    LacO3O1 and tetA promoters

    Available Since

    Oct. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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