We narrowed to 9,845 results for: Coli

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pColdI-DDX3_helicase_core_Q360P

Plasmid

#233620

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Pro (DDX3X Human)

Tags

His6

Expression

Bacterial

Mutation

Gln360Pro

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M5

Plasmid

#204563

Purpose

Expresses GST-tagged UFBP1 M5 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

215-314aa

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M3

Plasmid

#204561

Purpose

Expresses GST-tagged UFBP1 M3 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

116-314aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 M4

Plasmid

#204562

Purpose

Expresses GST-tagged UFBP1 M4 in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

116-214aa

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6p-1-UFBP1 F196A V198A

Plasmid

#204564

Purpose

Expresses GST-tagged UFBP1 F196A V198A in E.coli

Depositor

Masaaki Komatsu

Article

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

UFBP1 (DDRGK1 Human)

Expression

Bacterial

Mutation

F196A V198A

Available Since

Aug. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pGEX6P-1-MVP

Plasmid

#204541

Purpose

Expresses GST-tagged MVP in E.coli

Depositor

Masaaki Komatsu

Article

Kurusu et al Dev Cell. 2023 May 9:S1534-5807(23)00191

Insert

MVP (MVP Human)

Expression

Bacterial

Mutation

None

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pETM30-C9ORF78

Plasmid

#193367

Purpose

Expression of C9ORF78 in E.coli with N-terminal His6-GST-tag which is cleavable with TEV protease

Depositor

Markus Wahl

Article

Bergfort et al Nat Commun. 2022 Mar 3;13(1):1132. doi:

Insert

C9ORF78 (C9orf78 Human)

Tags

His6-GST-TEV

Expression

Bacterial

Promoter

T7

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-T76D

Plasmid

#175051

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunit 5 (RFC2, RFC3, RFC4, RFC5 Human)

Expression

Bacterial

Mutation

changed Thr76 to Asp, phosphomimetic mutation

Promoter

T7 promoter

Available Since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

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p36-p37-p38-p40-pET-Duet-1-Rfc3-3KtoA

Plasmid

#175050

Purpose

Overexpression of human Rfc2,Rfc3-3KtoA,Rfc4,Rfc5 in E.coli

Depositor

Brian Kelch

Article

Gaubitz et al Proc Natl Acad Sci U S A. 2020 Sep 22;11

Insert

Replication factor C subunits 2-5, Rfc3-3KtoA-mutation (RFC4, RFC2, RFC3, RFC5 Human)

Expression

Bacterial

Promoter

T7 promoter

Available Since

Sept. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pETM33_Nsp3e_NAB

Plasmid

#156473

Purpose

Bacterial expression of Sars-CoV2 Nsp3e_NAB protein with His-tag and GST-tag

Depositor

Ylva Ivarsson

Article

Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:

Insert

Nsp3e_NAB (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)

Tags

His-GST

Expression

Bacterial

Mutation

Gene insert is codon optimized for Ecoli.

Promoter

T7/LacO

Available Since

Aug. 4, 2020

Availability

Academic Institutions and Nonprofits only

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