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pUPD3_FULLPRM_Ptet

Plasmid

#243914

Purpose

GoldenBraid2.0 Tetracycline/Anhydrotetracycline-Inducible promoter, including ribosome binding site. Lacks repressor, TetR.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PtetA without tetR

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pUPD3_FULLPRM_PR

Plasmid

#243916

Purpose

GoldenBraid2.0 Temperature-Inducible promoter, including ribosome binding site. Lacks repressor, cI857.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

PR without cI857

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_FRTampFRT

Plasmid

#243898

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

bla

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_FRTkanFRT

Plasmid

#243899

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aph

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pR6Ko1_FRTspcFRT

Plasmid

#243902

Purpose

GoldenBraid2.0 Flp recombinase excisable antibiotic resistance cassette in the grammar of an assembled transcription unit. Ready to be amplified to make gene knockouts.

Depositor

Christophe Herman

Article

Cooke et al Synth Biol (Oxf). 2025 Aug 14;10(1):ysaf

Insert

aadA

Expression

Bacterial

Available Since

Jan. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pBL155

Plasmid

#246591

Purpose

express cCadC AtRbcL fusion protein

Depositor

Tina Wang

Article

Li et al Proc Natl Acad Sci U S A. 2025 Sep 16;12

Insert

cCadC-AtRbcL (rbcL Synthetic)

Expression

Bacterial

Available Since

Nov. 26, 2025

Availability

Academic Institutions and Nonprofits only

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pFerCA

Plasmid

#241903

Purpose

Part 1 of biosensor able to detect 13 substituted cinnamic acid based compounds

Depositor

Neil Dixon

Article

Machado et al Chem Commun (Camb). 2016 Sep 15;52(76):1

Insert

ferA and ferC

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pRM16

Plasmid

#174787

Purpose

Constitutive expression of the λCI protein; used as three-hybrid control corresponding to pCW17 (addgene #174664)

Depositor

Katherine Berry

Article

Stockert et al Nat Protoc. 2022 Apr;17(4):941-961. doi:

Insert

none - empty vector

Tags

λCI

Expression

Bacterial

Available Since

Aug. 8, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5415

Plasmid

#229030

Purpose

Expression vector for 10xHis-phi29 DNA polymerase (wild-type): PT7-lacO-g10leader-p2 wt-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_wt

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTE5444

Plasmid

#231064

Purpose

Expression vector for 10x-phi29 DNA polymerase (PovSak): PT7-lacO-g10leader-10xHis-p2 povsak-T7 terminator, CamR

Depositor

Tobias Erb

Article

Barthel et al bioRxiv 2025.07.08.663768; doi: 10.1101/

Insert

PT7-lacO-g10lead-10xHisTag-phi29 DNAP_PovSak

Tags

10x His-tag + Factor Xa recognition site

Expression

Bacterial

Promoter

PT7-lacO and T7 promoter

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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