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piHGpd/P82

Plasmid

#1325

Depositor

Susan Lindquist

Article

Nathan et al Proc Natl Acad Sci U S A 1997 Nov 25;94(

Insert

GPD promoter-HSP82 (HSP82 Budding Yeast)

Expression

Yeast

Mutation

Wild type gene

Available Since

Jan. 6, 2005

Availability

Academic Institutions and Nonprofits only
pLCeGFP

Plasmid

#241904

Purpose

Part 2 of biosensor able to detect 13 substituted cinnamic acid based compounds

Depositor

Neil Dixon

Article

Machado et al Chem Commun (Camb). 2016 Sep 15;52(76):1

Insert

plc, His6-eGFP

Use

Synthetic Biology

Mutation

none

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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AdeC-pET21b

Plasmid

#218482

Purpose

Expresses adenine deaminase in E. coli with a C-terminal his tag

Depositor

Audrey Lamb

Article

Pasin et al Methods Enzymol. 2024;702:51-74. doi: 10

Insert

Adenine deaminase

Tags

His tag

Expression

Bacterial

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

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pSLB025

Plasmid

#234634

Purpose

pET-28a(+) based plasmid for expression of H16_A1358 from Cupriavidus necator H16, with N-terminal hexahistidine tag

Depositor

Gregg Beckham

Article

Holmes et al Appl Environ Microbiol. 2025 Jun 12:e005

Insert

H16_A1358

Tags

6x-His

Expression

Bacterial

Available Since

June 10, 2025

Availability

Academic Institutions and Nonprofits only

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pGlomyc-Grhl2

Plasmid

#236050

Purpose

Mammalian expression vector with myc-tagged mouse Grhl2 coding sequence

Depositor

Renee van Amerongen

Insert

Grhl2 (Grhl2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-S-13xLexAop-GCaMP8m_VK05

Plasmid

#238557

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Sparse (S) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

TA et al Cell, CELL-D-24-04567, CELL13979

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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pHD-SPARC2-I-13xLexAop-GCaMP8m_VK05

Plasmid

#238556

Purpose

A LexAop-based SPARC plasmid for SPARC-L. Can also be used as a standalone sparsener of a LexA driver line. Intermediate (I) density. The GCaMP ORF can be swapped for your gene of interest.

Depositor

Tom Clandinin

Article

TA et al Cell, CELL-D-24-04567, CELL13979

Insert

GCaMP8m

Use

Sparc plasmid

Tags

DsRed-Express

Expression

Insect

Available Since

May 23, 2025

Availability

Academic Institutions and Nonprofits only

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RT-3

Plasmid

#220440

Purpose

Base construct used to create the PUF3/PUP2 tagging strain. (aka pCS2/3HA/PUM1_RBD/PUP2/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Promoter

MV IE93 promoter

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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RT-2

Plasmid

#220438

Purpose

Base construct used to create PUP2-DADA/3HA fusion proteins in S. cerevisiae. (aka pBSII/PUP2-DADA/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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RT-1

Plasmid

#220434

Purpose

Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Expression

Yeast

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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