We narrowed to 2,160 results for: Xpa

1. FUW-tetO-YAPS94A

   Plasmid

   #84010

   Purpose

   expresses a TEAD-binding mutant of YAP (YAPS94A) in mammalian cells under the control of a doxycycline-inducible promoter

   Depositor

   Stefano Piccolo

   Article

   Panciera et al Cell Stem Cell. 2016 Sep 9. pii: S1934-5

   Insert

   YAP1 (siRNA insensitive) (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   S94A

   Promoter

   TRE-CMV

   Available since

   Oct. 31, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. AAV2 ITR_phU6_gRNA scaffold-DMD sg1_phU6_gRNA scaffold-DMD sg16_pMHCK7-3x Flag-NLS-ecDHFR-enOsCas12f1-NLS-ecDHFR_pA_AAV2 ITR

   Plasmid

   #197029

   Purpose

   AAV vector encoding a human codon-optimized ecDHFR-enOsCas12f1-ecDHFR driven by MHCK7 promoter, DMD-targeting guide RNAs compatible with enOsCas12f1 driven by hU6

   Depositor

   Xiangfeng Kong

   Article

   Kong et al Nat Commun. 2023 Apr 11;14(1):2046. doi:

   Insert

   humanized ecDHFR-enOsCas12f1-ecDHFR
   

   Use

   AAV

   Tags

   Expression

   Mutation

   D52R / T132R

   Promoter

   MHCK7, hU6

   Available since

   May 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-CAG-NOTCH2NL-delta EGF repeats (N2NL-delta EGF)-ires-EGFP

   Plasmid

   #122955

   Purpose

   Lentiviral expression of NOTCH2NL delta EGF repeats under CAG promoter with EGFP expression

   Depositor

   Pierre Vanderhaeghen

   Article

   Suzuki et al Cell. 2018 May 31;173(6):1370-1384.e16.

   Insert

   NOTCH2NL-delta EGF repeats (NOTCH2NLB Human)
   

   Use

   Lentiviral

   Tags

   6xhis tag

   Expression

   Mutation

   deletion of EGF repeats from full length NOTCH2NLB

   Promoter

   CAG promoter

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAB(EXPR-DS-HDAC5_HDAC3)

   Plasmid

   #114399

   Purpose

   For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

   Depositor

   Vincent Dion

   Article

   Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

   Insert

   PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)
   

   Use

   Tags

   3xFlag-NLS (internal)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV CCTG 1200x

   Plasmid

   #63088

   Purpose

   AdenoAssociated Virus plasmid containing expanded repeats--CCTG 1200x that correspond to Myotonic Dystrophy of type 2

   Depositor

   Nicolas Charlet-Berguerand

   Insert

   CCUG 1200x
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCI_FLAG-GFP(Y39TAG)-6His

   Plasmid

   #223507

   Purpose

   Fluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39

   Depositor

   Edward Lemke

   Insert

   GFP(Y39TAG)
   

   Use

   Tags

   6xHis and FLAG

   Expression

   Mammalian

   Mutation

   Y39TAG

   Promoter

   Available since

   Oct. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. MUM1

   Plasmid

   #28273

   Depositor

   Cheryl Arrowsmith

   Insert

   MUM1 (MUM1 Human)
   

   Use

   Tags

   His with TEV cleavage

   Expression

   Bacterial

   Mutation

   Contains PWWP Domain (residues 405-538).

   Promoter

   Available since

   Feb. 10, 2012

   Availability

   Industry, Academic Institutions, and Nonprofits

8. pMAH2-CageCys

   Plasmid

   #71404

   Purpose

   Expresses tRNA and synthetase for mammalian encoding of a photocaged cysteine

   Depositor

   Huiwang Ai

   Article

   Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

   Insert

   aminoacyl-tRNA synthetase for a caged cysteine
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

9. pAS_MmaPylT_EF1_NES-MmaPylRS WT

   Plasmid

   #174901

   Purpose

   expression of Mma PylRS with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

   Depositor

   Simon Elsaesser

   Insert

   Mma PylRS (pylS Methanosarcina mazei)
   

   Use

   Tags

   FLAG and NES

   Expression

   Mammalian

   Mutation

   Promoter

   EF1

   Available since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMSCV-IRES-CFP II (pMIC II)

    Plasmid

    #52109

    Purpose

    a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV-IRES-DsRed FP (pMIDsRed II)

    Plasmid

    #52110

    Purpose

    a bicistronic IRES-DsRed containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-DsRed

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 9, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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12. pMSCV-IRES-YFP II (pMIY II)

    Plasmid

    #52108

    Purpose

    a bicistronic IRES-YFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_MmaPylT_EF1_NES-MmaPylRS AF

    Plasmid

    #174902

    Purpose

    expression of Mma PylRS AF with nuclear export sequence for amber suppression in mammalian cells; transient or piggy bac mediated integration

    Depositor

    Simon Elsaesser

    Insert

    Mma PylRS (pylS Methanosarcina mazei)
    

    Use

    Tags

    FLAG and NES

    Expression

    Mammalian

    Mutation

    Y306A, Y384F

    Promoter

    EF1

    Available since

    Nov. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV-IRES-GFP II (pMIG II)

    Plasmid

    #52107

    Purpose

    a bicistronic IRES-GFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

    Depositor

    Dario Vignali

    Article

    Holst et al Nat Protoc. 2006;1(1):406-17.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    IRES-EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pULTRA-CNF

    Plasmid

    #48215

    Purpose

    highly efficient suppressor plasmid

    Depositor

    Peter Schultz

    Article

    Schultz et al J Am Chem Soc. 2006 Nov 1;128(43):13984-

    Inserts

    polyspecific MJ tyrosyl synthetase
    tRNA
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32L, L65V, F108W, Q109M, D158G, I159A

    Promoter

    proK and tacI

    Available since

    June 1, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. iD20 LoxStopLox-Zim3-dCas9-mApple-hygro

    Plasmid

    #204720

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p3E-CMVmin:GFPmem pA

    Plasmid

    #80823

    Purpose

    CMVmin-promoted GFPmem with pA

    Depositor

    Philip Washbourne

    Article

    Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

    Insert

    3E-CMV-GFPCAAXpA spacer
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 1, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pRSF-G1(CysCNP)RS

    Plasmid

    #225054

    Purpose

    tRNA synthetase/tRNA pair for the incorporation of S-(4-cyanopyridin-2-yl)-L-cysteine (CysCNP) and S-(4-cyanopyrimidin-2-yl)-L-cysteine (CysCNPym) into proteins in response to the amber (TAG) codon

    Depositor

    Thomas Huber

    Article

    Abdelkader et al Chem 102385

    Insert

    tRNA synthetase
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Jan. 7, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pEVOL-pAzF

    Plasmid

    #31186

    Purpose

    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG

    Depositor

    Peter Schultz

    Article

    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7

    Insert

    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Y32T, E107N, D158P, I159L, L162Q, D286R

    Promoter

    Available since

    Dec. 9, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRF0G-Tyr

    Plasmid

    #113644

    Purpose

    Plasmid contains the aminoacyl-tRNA synthetase and tRNA genes from methanococcus jannaschii for charging tyrosine at amber stop codons.

    Depositor

    Jeffrey Barrick

    Article

    Monk et al ACS Synth Biol. 2016 Sep 20.

    Insert

    MjYRS and tRNA gene
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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