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We narrowed to 38 results for: 22894

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Showing: 1 - 20 of 38 results

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  1. pMRX-IP-EGFP-PA-BAX(mito)

    Plasmid
    #228942
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-OMP25 in mammalian cells. Photoactivatable BAX localizes on the outer mitochondrial membrane and induces mitochondrial rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Omp25 (Synj2bp Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228942-plasmid-map-sequence-id-466826

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  2. pMRX-IP-EGFP-PA-BAX(ER)

    Plasmid
    #228941
    Purpose
    Stable expression of EGFP-LOV2(N538E)-BAX-Cyb5a in mammalian cells. Photoactivatable BAX localizes on ER and induces ER rupture upon blue light stimulation.
    Depositor
    Noboru Mizushima
    Article
    Nagashima et al J Biol Chem. 2025 Mar 18;301(4):108421.
    Inserts
    LOV2 domain of phototropin1
    BAX (BAX Human)
    Cyb5a (Cyb5a Rat)
    Use
    Retroviral
    Tags
    EGFP
    Expression
    Mammalian
    Mutation
    N-terminus and C-terminus are deleted. BAX(3-171)…
    Available Since
    April 28, 2025
    Availability
    Academic Institutions and Nonprofits only
    228941-plasmid-map-sequence-id-469413

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  3. pET28a-UBC9

    Plasmid
    #228949
    Purpose
    express a fragment of the wild-type human UBC9 (residues 2-158 of UniProt P63279 supplemented with the N-terminal thrombin-cleavable MGHHHHHHSSGLVPR|G- tag)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228949-plasmid-map-sequence-id-472392

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  4. pET28a-UBC9-K14R-A129K

    Plasmid
    #228948
    Purpose
    express a fragment of the double mutant of the human UBC9 (generated from pET28-UBC9 through mutagenesis; point mutations: K14R and A129K)
    Depositor
    Marcin Suskiewicz
    Insert
    UBC9 (UBE2I Human)
    Tags
    His tag and thrombine cleavage site : MGHHHHHHSSG…
    Expression
    Bacterial
    Mutation
    changed Lysine 14 to Arginine, changed Alanine 12…
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228948-plasmid-map-sequence-id-472389

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  5. pET28a-RANGAP1-CTD

    Plasmid
    #228947
    Purpose
    express the C-terminal domain of the human RANGAP1 protein (residues 418-587 of UniProt P46060 with the N-terminal thrombin-cleavable MGSSHHHHHHSSGLVPR|GSHM- tag). Codon-optimized
    Depositor
    Marcin Suskiewicz
    Insert
    RANGAP1-CTD (RANGAP1 Human)
    Tags
    His tag and thrombine cleavage site : MGSSHHHHHHS…
    Expression
    Bacterial
    Mutation
    contains aa 418-587 only
    Promoter
    T7-lac
    Available Since
    May 13, 2025
    Availability
    Academic Institutions and Nonprofits only
    228947-plasmid-map-sequence-id-472386

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  7. pcDNA3.1-MS2-HA-HsDIS3_Z

    Plasmid
    #148111
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Available Since
    March 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    148111-plasmid-map-sequence-id-407051

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  8. pT7-V5-SBP-C1-HsDIS3_Z

    Plasmid
    #148137
    Purpose
    Mammalian Expression of HsDIS3
    Depositor
    Elisa Izaurralde
    Insert
    HsDIS3 (DIS3 Human)
    Expression
    Mammalian
    Mutation
    L760F compared to the sequence given by isoform 2…
    Available Since
    Nov. 1, 2022
    Availability
    Academic Institutions and Nonprofits only
    148137-plasmid-map-sequence-id-453676

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  12. L2373-FRB-GPA-20-114

    Plasmid
    #222894
    Purpose
    Biosensor chain detecting rapamycin and responding with split Nanoluciferase reconstitution. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, split Nanoluciferase fragment 114.
    Depositor
    Joshua Leonard
    Article
    Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1
    Insert
    FRB-GPA-20GS-NanoLuc114
    Tags
    Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 12, 2024
    Availability
    Academic Institutions and Nonprofits only
    222894-plasmid-map-sequence-id-440207

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  13. pCMV6-XL4 FLAG-CBLN4-Fc

    Plasmid
    #115769
    Purpose
    Expression of the extracellular domain of CBLN4 with N-terminal FLAG tag and C-terminal hIgG tag.
    Depositor
    Davide Comoletti
    Insert
    CBLN4 (CBLN4 Human)
    Tags
    FLAG and hIgG/Fc
    Expression
    Mammalian
    Mutation
    none
    Promoter
    CMV
    Available Since
    Oct. 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    115769-plasmid-map-sequence-id-223827

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Showing: 1 - 20 of 38 results