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pCD86-mEYFP(Q69K)

Plasmid

#190746

Purpose

Expression of mEYFP(Q69K)-tagged CD86 in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

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pmCherry2-CD86-mEYFP(Q69K)

Plasmid

#190749

Purpose

Expression of CD86 double tagged with mCherry2 (N-term) and mEYFP(Q69K) (C-term) in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCD86-mEYFP(Q69K)-mEGFP

Plasmid

#190750

Purpose

Expression of CD86 double tagged with mEYFP(Q69K) and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mEGFP and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry2-CD86-mEYFP(Q69K)-mEGFP

Plasmid

#190751

Purpose

Expression of triple tagged CD86 with mCherry2, mEYFP(Q69K), and mEGFP in mammalian cells.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Insert

CD86 (CD86 Human)

Use

Tags

mCherry2, mEGFP, and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Sept. 27, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pmCherry2-GNB1-T2A-mCherry2-GNG2-IRES-GNAI1-mEYFP(Q69K)

Plasmid

#190755

Purpose

Expression of trimeric G protein with mCherry2 (GNB1 and GNG2) or mEYFP(Q69K) (GNAI1) tags.

Depositor

Dave Piston

Article

Foust et al bioRxiv 2021.11.03.467169

Inserts

GNB1 (GNB1 Human)
GNG2 (GNG2 Human)
GNAI1 (GNAI1 Human)

Use

Tags

mCherry2 and mEYFP(Q69K)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 18, 2022

Availability

Academic Institutions and Nonprofits only

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mClY-N1

Plasmid

#90457

Purpose

A YFP-based chloride sensor with enhanced chloride sensitivity, photostability and reduced pH interference. The sequence of mClY is EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206K.

Depositor

Joseph Santos-Sacchi

Article

Zhong et al PLoS One. 2014 Jun 5;9(6):e99095. doi: 1

Insert

mClY-N1

Use

Tags

Expression

Mammalian

Mutation

EYFP-F46L/Q69K/H148Q/I152L/V163S/S175G/S205V/A206…

Promoter

Available since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge