We narrowed to 78 results for: FreeGenes

#165521

Operon containing Exo, Bet, and Gam. To use the lambda red recombineering system to modify your target DNA.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 3, 2021

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#165501

Robust and extremely thermostable polymerase with 5x higher fidelity than Taq. Ideal for GC-rich or looped sequences.

Drew Endy, Jennifer Molloy, FreeGenes Project

July 26, 2021

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#165573

DNA polymerase responsible for protein-primed viral DNA replication by strand displacement with high processivity and fidelity. Possesses three enzymatic activities: DNA synthesis (polymerase), primer terminal protein (TP) deoxynucleotidylation, and 3' to 5' exonuclease activity.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 24, 2021

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#165556

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity. Comparable to SuperScriptII from Thermo Fisher.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 16, 2021

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#165520

The most commonly used polynucleotide kinase.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 3, 2021

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#165559

Double-stranded DNA specific exonuclease and single-stranded DNA endonuclease. Initiates at the 5' termini of linear or nicked double-stranded DNA. Cleaves linear or nicked double-stranded DNA in the 5' to 3' direction.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 16, 2021

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#165529

Used to digest methylated DNA; to be expressed in dam- E. coli strains (strains which do not methylate their genomic DNA)

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 5, 2021

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#165572

Robust and extremely thermostable polymerase with a half-life of 23 hours at 95 degrees C; offers 5x higher fidelity than Taq and robust performance. Ideal for GC-rich or looped sequences. Lacks exonuclease activity. Comparable to Deep Vent (exo-) DNA Polymerase at NEB

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 24, 2021

Industry, Academic Institutions, and Nonprofits

#165546

Single 75 kDa monomer, cDNA synthesis; high enzyme activity and processivity.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 10, 2021

Industry, Academic Institutions, and Nonprofits

#165570

Significantly improves maximum amplicon size and PCR yields for Family B polymerases like Pfu or Phusion. Comparable to ArchaeMaxx/PfuTurbo.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 23, 2021

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#165549

ssDNA binding protein, 9kDa. Features: Improves the processivity of DNA polymerase; involved in stabilization and marking of ssDNA structure; increases the yield and specificitiy of PCR; increases the yield and processivity of RT during RT-PCR; improves DNA sequencing through regions with strong secondary structure

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 10, 2021

Industry, Academic Institutions, and Nonprofits

#165569

Taq DNA Ligase

Drew Endy, Jennifer Molloy, FreeGenes Project

Oct. 25, 2021

Industry, Academic Institutions, and Nonprofits

#165509

Used for site-specific cleavage of proteins. TEV cleaves the protein consensus sequence EXXYXQ^S/G

Drew Endy, Jennifer Molloy, FreeGenes Project

July 30, 2021

Industry, Academic Institutions, and Nonprofits

#165516

DNA-dependent RNA polymerase derived from SP6 bacteriophage that catalyzes the transcription of viral genes.

Drew Endy, Jennifer Molloy, FreeGenes Project

July 29, 2021

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#165565

Ribonuclease A or RNase A; endoribonuclease purified from bovine pancreas. Important enzyme for the removal of RNA for RNA free DNA purification reactions such as plasmid DNA purification and genomic DNA purification, RNA removal from recombinant protein preparations, ribonuclease protection assays, mapping single-base mutations in DNA/RNA.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 17, 2021

Industry, Academic Institutions, and Nonprofits

#165504

Type II restriction enzyme that recognizes the double-stranded sequence GAATTC and cleaves after G-1.

Drew Endy, Jennifer Molloy, FreeGenes Project

July 30, 2021

Industry, Academic Institutions, and Nonprofits

#165505

Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

Drew Endy, Jennifer Molloy, FreeGenes Project

July 27, 2021

Industry, Academic Institutions, and Nonprofits

#165548

Type II restriction enzyme that recognizes the double-stranded sequence GATATC and cleaves after T-1.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 10, 2021

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#165539

High fidelity strand displacing polymerase. Allows one to amplify complex/problematic sequences.

Drew Endy, Jennifer Molloy, FreeGenes Project

Sept. 1, 2021

Industry, Academic Institutions, and Nonprofits

#165524

Polymerase that replicates viral genomic DNA.

Drew Endy, Jennifer Molloy, FreeGenes Project

Aug. 4, 2021

Industry, Academic Institutions, and Nonprofits