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  1. Science Communication for Everyone

    Type
    Blog Post
    ... was founded in early 2018 with the goal of communicating science to non-scientists. Chinmaya Sadangi,...questions about The Addictive Brain and science communication for general audiences. How does The Addictive...Addictive Brain address science communication? At The Addictive Brain, we collaborate with scientists from around...sci-art.  What are your top tips for science communication? It is important to identify your audience ...on the Addgene blog Find all of our science communication posts Sign up to receive our science career ...guide Learn about different careers in science communication Resources on Addgene.org Learn how Addgene...

  2. Pooled Library Amplifications

    Type
    Blog Post
    ...ask for an amplification protocol with your deposit. This is because repeated amplifications of pooled ... an amplification protocol developed for each individual pooled library; generalized amplification protocols...Plasmids Variable Region Size DNA per Amplification Amplification Type Link to Protocol CRISPR Knockout... this blog, we’ll discuss how to develop an amplification protocol for your pooled library deposit. When...to amplify the sample for further downstream applications. As individual libraries can vary significantly... to consider when creating a pooled library amplification protocol. Created with BioRender.com.   ...Libraries Protocol.   Selecting your strain for amplification Since pooled libraries often contain thousands...

  3. Plasmids 101: Origin of Replication

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    Blog Post
    ...the origin of replication/replicon. The replicon is comprised of the origin of replication (ori) and all...   Types of origins of replication There are lots of origins of replication out there so, for simplicity...gene for replication (reference). **F1 is a phage-derived ori that allows for the replication and packaging...control elements. The ori is the place where DNA replication begins, enabling a plasmid to reproduce itself...are generally different from the those used to replicate the host's chromosomal DNA, but they still rely...readily at lower temperatures and allows the replication machinery room to come in and get busy making...derivatives), pSC101, R6K, and 15A. Not all origins of replication are created equal. Some will produce many plasmid...

  4. Fostering communication in remote collaborations

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    Blog Post
    ...researchers involved. Communication is a major challenge when researchers are communicating across disciplines...closely replicates how you’d interact with team members in person, and improves overall communication.  Maintain...Furthermore, this casual communication reflects the type of informal communication you’d likely have with...But these challenges can be exaggerated when communicating across time zones, digitally, and asynchronously...to keep everyone on the same page is to make communication a non-negotiable part of your collaboration....great way to include researchers from different locations. Having virtual face-to-face meetings as opposed... Maintain informal communication In between recurring meetings, you should share interesting results or...

  5. PhD Applications After COVID

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    Blog Post
    ...previous nosy question! The Application Process Broadly speaking, the PhD application process tends to go something...steps in the application process and usually occurs in the few months leading up to applications opening. ...program. Most applications will recommend that you name two professors in your application that you intend...to continue the application process through a campus visit, a weekend where applications can get to know...strengthen your application and help you decide if a program is a good fit for you. The application process looks...next?” For those finishing up an undergraduate education or ending a Master’s program, the answer might...know that this process only became even more complicated with COVID-19 and standards for hybrid, virtual...

  6. DNA Purification Without a Kit

    Type
    Blog Post
    ... DNA purification without a kit as well as one way to reuse silica columns from DNA purification kits....consider some ways to purify DNA without a kit. DNA purification kits have advantages: they are convenient and...because you’ve run out of columns. Kit-less DNA purification methods avoid many of the drawbacks of kits ... and then rehydrated. There are some slight modifications depending on the type of DNA you’re purifying...After this, the DNA is ready for use! Plasmid purification and DNA gel extraction with glass syringe filters...Product: plasmids or DNA fragments The plasmid purification and DNA gel extraction method described in Kim...salt (guanidine hydrochloride for the plasmid purification protocol and guanidine isothiocyanate for the...

  7. Truncated gRNAs for Regulating Gene Expression

    Type
    Blog Post
    ...target site. Benefits of truncated gRNAs Importantly, the use of truncated guides has largely been demonstrated...protospacer, in the genome. Native Cas9 has two catalytic domains, each of which cleaves one strand of DNA...gRNA (20nt complementarity to target site) or truncated gRNA (≤15nt complementarity to target site). When...complexed with a full-length gRNA, native Cas9's two catalytic domains, HNH and RuvC, cleave DNA strands complementary...the gRNA, respectively. When complexed with a truncated gRNA, Cas9 binds the target site, but does not... addition to its utility in making targeted modifications to DNA, Cas9 can be reprogrammed to serve as...as a regulator of gene expression. Its catalytic domains can be mutated to inactivate the protein’s nucleolytic...

  8. Summer SciComm Series: Modes of Communication

    Type
    Blog Post
    ...xkcd.com Science communication should be fun and engaging for the communicator as well as the audience...many ways options available to today's science communications: podcasts, blogging, videos, lectures, exhibits...in what people think of as "the best way" to communicate their science, meaning that they will heavily...heavily evangelize for the mode of communication they connect best to. And that's really natural - if it works... text or use Facebook. However you choose to communicate your science, some people will love it and others...platforms we use at Addgene: YouTube, for longer educational videos, and Twitter, for sharing updates, new...factors like socioeconomic status and geographic location. For example, not all social media platforms are...

  9. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    ...extension is repeated 25-35 times to exponentially replicate the target DNA of interest. This entire reaction...have been developed for different scientific applications. These PCR methods all use the same basic PCR...the final cycles which would slow or stop PCR amplification. Other times, the PCR reaction may not be 100%...quantified by qPCR. qPCR is used in a variety of applications including gene expression profiling, studying...concentration, unlike qPCR where results can vary across replicates. ddPCR can be used to quantify DNA sequences ...droplet contains the material required for PCR amplification. Following the PCR reactions each droplet is... our blog post “Droplet Digital PCR for AAV Quantification.”   Multiplex PCR Multiplex PCR, as the name...

  10. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...plasmids rely on their host cell's replication machinery in order to replicate—but not always to the same extent...described in our previous Origin of Replication post, DNA replication is initiated at the ori and may or...consider the replication mechanism of plasmids under stringent control, such as pSC101. DNA replication and regulation...review how E. coli normally replicate their chromosomal DNA. Chromosomal replication in bacteria is carefully...entire round of replication. Initiation is the primary control point of DNA replication in bacteria, so... the replication forks reach each other on the opposite end of the chromosome, ending replication.   ...chromosomal replication and balance population growth against sustainability. Plasmid DNA replication and regulation...

  11. Selecting Your Plasmid Purification Kit

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    Blog Post
    ...column-based DNA purification methods perform well in terms of quality and yield. Bead-based purifications tend to...standard purification kits, there’s a range of specialty kits available for plasmid purification. Kits can...optimized for purification of these large plasmids, while others have suggested modifications to their normal...you’re new to using purification kits, or if you’re struggling with a purification, reading the full instruction...molecular biology. And while kit-free plasmid purification is always an option, let’s be honest — kits ... plasmid preps are the same. Your downstream application will dictate how much DNA you need to recover...recover and the level of quality required. Some applications require very little DNA and you can get away with...

  12. Viral Vectors 101: Viral Applications

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    Blog Post
    ...their use in other clinical applications. In the last decade, viral applications in cancer treatment and ...s unpack the myriad number of emerging viral applications and give you some recommendations for putting...putting viruses to work. Viral applications Viruses work by infecting a host cell (the target of viral infection...expressed. It’s important to note that for all the applications we discuss, there is more than one type of virus...retrovirus, AAV, etc.). When you’ve decided on an application, our Viruses as Biological Tools blog can help...viruses and pick one for your experiment. Research applications Gene expression control Researchers are always...expression control mechanisms require a specific modification of the viral vector for your gene of interest...

  13. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    ...Voltage indicators are voltmeters for cells! In this blog we will review how these indicators work, their...fluorescence or voltage indicators.   Viral Vectors and Voltage Indicators As previously mentioned...Enhanced genetically encoded voltage indicators advance their applications in neuroscience. Curr Opin Biomed...Cellular communication and function depend on potential energy. Cellular activities like action potentials...their general applications, and why viruses are essential to their use as biological tools. Why Use Voltage...Voltage Indicators? Electrical signals are integral to many facets of biology. For example, many heart...within a cell are genetically encoded voltage indicators (GEVIs) and chemigenetic sensors. These tools...

  14. CasPEDIA: A Functional Classification of Cas Enzymes

    Type
    Blog Post
    ...established uses, and intended applications. Within CasPEDIA, there is a classification system, CasID, to help...multiplex ability Experimental guidelines and applications Protein structure and properties Links to ...you want to read literature on the less common applications of an enzyme for your non-traditional experiment...al. (2023). CasPEDIA Database: A Functional Classification System for Class 2 CRISPR-Cas Enzymes [Preprint...I., Iranzo, J., et al. (2019). Evolutionary classification of CRISPR–Cas systems: a burst of class 2 and...

  15. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    ...after application of NE (10 μM) and corresponding pixel-wise ΔF/F0 heatmaps. White insets indicate surface...developing and depositing two new norepinephrine indicators: nLightG and nLightR (Kagiampaki et al., 2023...surface expression of the indicators over white dashed lines. Scale bars, 10 μm (HEK293T), 20 μm (neurons...trialed in two-photon in vivo experiments. nLight indicators are more sensitive to and selective for norepinephrine...They can, however, be studied with the nLight indicators. Speed One of the major advantages of this next...… Patriarchi, T. (2023). Sensitive multicolor indicators for monitoring norepinephrine in vivo. Nature...

  16. 5 Reasons to Use Reddit for Science Communication

    Type
    Blog Post
    ...re more interested in selling a product than communicating your ideas with people or discussing science...to write about in the future. The AMA was also cataloged for permanent reference by the Winnower. The only...I do a lot of public speaking where I enjoy communicating face to face with the audience. I post on LinkedIn...own expertise and your resources for science communication opportunities like an AMA. One of the best things... science communication...

  17. Easing the Protein Purification Process with pCri

    Type
    Blog Post
    ...protein purification. For a more thorough introduction to pCri, please see the original publication cited...expression and purification: a comprehensive review of affinity tags and microbial applications." Biotechnology... Protein purification can be one of the most stressful lab activities. Working with proteins requires...interest can save a lot of time later. Protein purification specialists recommend doing some research, notably...Gomis-Rüth, aims to take the guesswork out of protein purification through simple and efficient multiplexing. pCri...sites should one need to remove the tag post-purification. A sample of pCri constructs: pCri-1a, -4a ...4a and -9a. 6xHis tags enable affinity purification using a Ni-NTA resin. MBP improves protein solubility...

  18. Science Communication Snapshot: DayCon 2015

    Type
    Blog Post
    ... Here at Addgene, we’re dedicated to advancing and sharing science! In association with the Harvard graduate...chief goals. First, SITN works to bridge the communication gap between scientists and the public and help... SITN helps graduate students develop their communication skills and engage with the public. The organization...students, these opportunities to improve our communication and organizational skills provide great training... science communication...

  19. New Educational Resource: The Addgene Videos Page

    Type
    Blog Post
    ...for communicating scientific knowledge. On this new page, we have neatly organized the educational video...plasmid repository, Addgene aims to be a vital educational resource for scientists around the world. With...

  20. New Tools Enable CRISPRa for Neuroscience Applications

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    Blog Post
    ...genetic underpinnings of these processes has been complicated by the lack of precise genetic tools for modulating...recent advancements and offer commentary on their application to neuroscience research. What is CRISPR-based...accessible to neuroscientists, not all neuroscience applications require such optimized tools. This is elegantly...necessitates such molecular gymnastics for sensitive applications such as CRISPR screening. The plasmids used ...used by the Qi Lab for this particular application can be found here.  Why CRISPRa? Although cDNA overexpression...gene activation in select in vitro and in vivo applications, the tools described above make CRISPRa in the...relatively easy and accessible. There are also other applications for which CRISPRa is well-suited, including ...

  21. Addgene Protocols Go Viral: AAV Purification and Titration

    Type
    Blog Post
    ...some tips for performing this purification.  Check out the AAV Purification protocol page for more detailed...! Subscribe to the Addgene YouTube page! AAV purification by iodixanol gradient ultracentrifugation This...This video goes over AAV vector purification using an iodixanol column gradient. We'll show the loading...

  22. Summer SciComm Series: A PhD in Science Communication

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    Blog Post
    ...Further, when science is communicated in a format that is accessible, that communication often neglects the ...SciCommSummer Scicomm Series: Modes of CommunicationCareers in Science Communication: Science Writing... to save a few days’ worth of capsules to self-medicate with when they get a cold. A primary school teacher...dihydrogen monoxide (H2O). Studying science communication as a PhD student Fortunately, by the time I ...Otago had established the Centre for Science communication and I was accepted as a grad student. I chose...a phenomenon called “motivated reasoning,” the catchall term for a range of neat little tricks our minds...found opportunities for more hands-on science communication. Between New Zealand’s multiple Covid-19 lockdowns...

  23. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    ...run in triplicate.  The nice thing about qPCR is that since it measures relative quantification - that... not need to be run in triplicates. ddPCR is an extremely accurate application, even in areas of the genome...Digital PCR for AAV QuantificationPolymerase Chain Reaction Overview and Applications Resources on Addgene.org...blog post, we're going to focus on PCR-based applications (qPCR and ddPCR.) qPCR Quantitative/real-time...range of RQ values as noted above.   In both applications, results can be affected by DNA quality and ...absolute quantitative data, instead of relative quantification.  Even though no one option is perfect, with...your variant options, you’re likely to find an application that matches your needs. Happy quantifying!  ...

  24. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...and applications.    Plant applications We outlined many synthetic biology MoClo applications, but...- and multi-gene constructs for a variety of applications. MoClo tools can be used in bacterial and cell-free...and the kits Addgene offers to kickstart these applications, no matter what your model organism is. MoClo...this blog, we will highlight some common MoClo applications and kits. Keep in mind that this technology ... it, MoClo might help you achieve it! MoClo applications and specialized tools Synthetic biology in bacteria...and multi-TU constructs for synthetic biology applications in bacteria or cell-free systems. The approach...combinations of promoters, terminators, solubility and purification tags, connectors, cDNAs, and more to find the...

  25. We're Updating Our Hold for Publication Policy

    Type
    Blog Post
    ...other scientists to use? We take this Hold for Publication policy seriously and appreciate the trust you...of July 1, 2025, we are updating our Hold for Publication policy with two changes that will help us ensure...hold if they are clearly described in a journal publication. Plasmids will be released 3 years after the ...keep things moving internally. And remember, a publication isn’t required to share your plasmids! You can...can make your deposit available before publication and we can update the plasmids’ citation when the paper...

  26. The Many Reasons for Irreproducible Research- and a Vaccine to Eradicate It

    Type
    Blog Post
    ...after credible attempts to replicate major findings could not be replicated by other researchers.There...published research is difficult to replicate.  Why is replicating previous research findings so difficult...can be difficult to replicate. Unclear methods Until someone attempts to replicate the results of another...positives in original studies and false negatives in replications are unavoidable, but can be minimized with high...obtaining a different result after conducting a replication study. An important difference between the original... methods, QRPs, and small samples, then when replications do not come out the same as an original research...data, materials, and code Only when you try to replicate the work of other researchers (or your own from...

  27. Transferable Skills Guide: Cross-team Communication

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    Blog Post
    .... I needed to a) figure out the sources of miscommunication and b) find practical solutions.  My PhD ... for this task. Good scientists learn how to communicate their work differently based on their audience... family is going to vary widely. The way you communicate your science at a poster is going to vary depending...two main problems with Addgene’s cross team communication. Although these real world examples did not ... for the container to be used in cross-team communication A list of “AKA” names, or jargon terms, for ...tracking system. Though you might not have to communicate with a team of software engineers in your lab...experimental protocols. Clarify: Asking for clarification during a meeting can help get everyone on the...

  28. Communicating Your Science With Help From ComSciCon

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    Blog Post
    ... the Communicating Science Conference series for graduate students. I believe that communication is the...skill in science communication over time.  Like most skills, great science communication comes with practice...able to communicate with their colleagues and peers. Scientists also need to be able to communicate to expert...need to communicate with non-scientists, to establish broader impacts for their work in education and policy...with opportunities to practice communicating.  Some fantastic K12 education initiatives like BiteScis (https...careers in science communication The strength of story telling Science communication: 9 strategies to get... world is the value that you can succeed in communicating. Even the most rigorous, insightful, and novel...

  29. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post
    ...science communication are as varied as the field itself. Among other things, science communication comprises...certain aspects of art and education. If you suspect that science communication might be for you, don’t ... science education Do you aim to reach young people? Try networking with your local education community... Training – Get a formal education Some people get into science communication by taking a media or journalism...project. Science communication opens doors Getting involved in science communication has been the most...career researcher looking to break into science communication? Everybody goes about this in their own way....t wait. Start communicating now. These 9 strategies will get you started:  Subscribe to Career Blog Posts...

  30. Educational Planarian Tools for Teaching Developmental Biology Techniques

    Type
    Blog Post
    ...DE3. For Phagocata morgani and Phagocata gracillis, the process is a little more complicated. These strains...Girardia sp., Dugesia dorotocephala, Phagocata morgani, and Phagocata gracillis. There are 11 different ... coli. Thus, to get the dsRNA into Phagocata morgani and Phagocata gracillis, one has to purify the dsRNA... in situ hybridization experiments. A recent publication [1] from the Sánchez lab describes the plasmids...the deposit) are described in the Sánchez lab publication [1] so that the students performing these experiments...mRNAs are subsequently destroyed by Argonaute-catalyzed nuclease activity or their translation disrupted...-amputation planarians after knockdown of beta-catenin. Other gene targets do not require amputation to...

  31. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post
    ...Riglar Advocating for Science Symposium and Workshop 2016 – Sept 16-17 MIT, Boston The Advocating for Science...futureofresearch.org/advocating-for-science-boston-2016/. Sign Up For the Advocating for Science Symposium...Director of Media Communications, Harvard University Ray Howell -Founder of Howell Communications Philip Brenner...union dues. Time will tell the extent of the implications of this ruling and whether graduate students...labor laws, rather than as trainees in a special category, as has been the ‘labor-of-love’ philosophy of...tight lab budgets and even simply being able to categorize all postdocs at institutions, will be challenging...Revisited” 2014 Appendix B p93-95). For those advocating in this area, keeping the pressure on for further...

  32. Experimenting in an Open Source lab: from CRISPR to Cats

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    Blog Post
    ... Working on the Cat Microbiome Project Another project we are working with is the cat microbiome project...data for analysis. And, well, cats... everyone knows the internet loves cats! This is mostly a project to...gut of the domesticated housecat, and seeing how that makeup differs from that of wild cats with the goal... goal of optimizing the health of domesticated cats and learning about feline microbiomes. One of the ...OpenWorm Project Website: http://www.openworm.org/ Cat Microbiome Project Website: http://www.kittybiome.com...biologists and computer scientists learning and communicating with one another.      Currently our main focus... of Minnesota has declared our makerspace an educational institution meaning that we can obtain known ...

  33. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post
    ...where readers may locate data supporting the research reported in PLOS publications. The policy was not... is founded on the principle that scientific publications must contain enough information (or links to...probable to some extent - but that is the price of publication. Who is to say that the initial data-generating...logically annotated and arranged, as in an actual publication and not require interaction with the data authors...likely, at least in part, due to our inadequate cataloguing of how results were obtained and this alone is... Nature Publishing Group recently launched a publication called Scientific Data, which aims to act as ...how they view the information. We are used to publications presenting a polished, standard and usually ...

  34. Quantifying DNA? Here are Five DNA Quantification Methods to Consider

    Type
    Blog Post
    ...see any signs of DNA in your final tube after purification. How do you know if you actually have DNA in...you choose could be based on your downstream application, time, and instrument availability. UV absorbance... at 230 nm. The A260/A280 ratio is used as an indicator of DNA purity. Ideally, this number should be ...like a PCR product). This method also gives an indicator of DNA or RNA contamination based on the presence...before larger fragments. Figure 1: The quantification of a RNA ladder using capillary electrophoresis... about quantifying DNA In choosing your DNA quantification method there’s many things to consider: cost...

  35. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post
    ...they asked me, 'How do you feel about dissecting a cat?' And I’m like, 'Well, I’m here so let’s do it!'"...in creative science education tools? Check out this Addgene podcast with educator and science rap mastermind...In this post of the Careers in Science Communication blog series, you’ll hear more about Caitlin Runne-Janczy...subject matter expert (SME) at eScience Labs, an educational company that creates hands-on science lab kits...jack-of-all-trades. “As a subject matter expert in the educational sphere, it can range from researching an area...take a step back and look at new technologies in education and ask ‘Can we implement that?’ [And think about...business.” Caitlin’s advice for budding science communicators: “My recommendation would be to take any opportunity...

  36. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post
    ...In this post in the Careers in Science Communication blog series, you’ll learn about Caitlin Runne-Janczy...Product Development Manager at eScience Labs, an educational company that creates hands-on science lab kits...through Caitlin’s blood. “I come from a family of educators, mostly elementary school, middle school, and ...where I came from.” Working at eScience Labs, an educational company that creates hands-on science lab kits...when it comes to the labs.” While her love of educational science writing is clear, Caitlin’s career plans...to do scientific editing.” It was for Cactus Communications, a company that provides scientific editing...can learn what it’s like to work for a science education company. And make sure to subscribe to the Career...

  37. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

    Type
    Blog Post
    ...about careers in science communication. When I started my Science Communication Internship with Addgene...ll interview three science communicators who work in the biotech, education, or nonprofit industries. ...re interested in learning more about science communication careers, you’re in the right place. For this...should be. He debated taking a position as an application support/sales representative in industry or a...job. “I am really more of a multimedia science communicator than a science writer these days. I do some ...illustrations for summarizing mechanisms and applications that are relevant to things IDT does. I also...next couple of months. This involves a lot of communication with the presenters, and editing of abstracts...

  38. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    ...scientists have long used antibody based affinity purification and mass spectrometry approaches. Recently however...that are tagged with biotin using avidin coated purification techniques. Due to the high affinity of biotin...challenge for classic antibody based affinity purification/mass spectrometry methods.  BioID: a promiscuous...can even target BirA* to specific subcellular locations, such as the nuclear envelope, allowing scientists...scientists to probe interactions in locations of interest.  Try BioID for your proximity labeling experiment... proximity labeling (Rhee et al., 2013). APEX catalyzes the oxidation of biotin-phenol to the short-lived...mutation (R40G) in a conserved residue in the biotin catalytic domain to allow for promiscuous biotinylation....

  39. Opto-Nanobodies: Using Light to Manipulate Cell Signaling and Protein Purification

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    Blog Post
    ...#1: Affinity tag purification You tag YFP and use an affinity column for purification. After binding YFP...  Light controlled protein purification with OptoNBs The first application for OptoNBs takes us in vitro...light-controlled affinity purification of unmodified proteins. Affinity purification methods typically require...It’s time to choose your own protein purification adventure. You want to purify your favorite protein...elute YFP.  Option #2: Opto-Nanobodies (OptoNBs) purification You skip adding a tag to YFP and instead use...sound like fiction, but light-controlled protein purification is now a reality thanks to OptoNBs. Recently...response to light allows for tagless protein purification as well as reversibly regulation of endogenous...

  40. Oh, The Places You Can Go: Careers in Science Communication - Writing for a Research Institute

    Type
    Blog Post
    ...things or communications planning.” To beef up her resume, she decided to complete a certificate in Strategic...Podcast Episode on Science Communication Check out Other Science Communication Posts Resources on Addgene.org...In this post of the Careers in Science Communication blog series, you’ll hear from Susan Keown, a staff...Cancer Research Center. Susan Keown’s science communication journey started with something unexpected: termites... time position with a Washington D.C.-based communications consulting firm, Palladian Partners. “I was...Strategic Communications and Public Relations at the University of Washington and the connections she made...storytelling is an impactful tool for science communicators. “The power of a story is to make a human connection...
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