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We narrowed to 57 results for: eomes

Showing: 1 - 50 of 57 results

  1. Mapping the 4D nucleome with CRISPR/Cas9

    Type
    Blog Post
    ...lab, brings us one step closer to mapping the 4D nucleome, the organization of the nucleus in space and ...loci, a major benchmark in characterizing the 4D nucleome. Future modifications and applications Ma et al...technique. The NIH Common Fund has made mapping the 4D nucleome a specific priority, as researchers work to increase... nucleome...

  2. Proximity Labeling: A Powerful Tool for Protein Complex Purification and Proteomic Mapping

    Type
    Blog Post
    .... APEX has allowed scientists to identify the proteomic map of the human mitochondrial matrix (Rhee et...TurboID and miniturbo Split-BioID: A conditional proteomics approach to monitor protein-protein interactions...Central PMCID: PMC3308701. Rhee, Hyun-Woo, et al. "Proteomic mapping of mitochondria in living cells via spatially...Isabel Myriam, et al. "Split-BioID a conditional proteomics approach to monitor the composition of spatiotemporally...

  3. Hot Plasmids: Fall 2025

    Type
    Blog Post
    ...FRCaMPi from the Kiryl Piatkevich Lab (Zhou, Zhu, Eom, Fang, et al., 2025). These new sensors use quite...neurons; scale bars 50 µm. Reproduced from Zhou, Zhu, Eom, Fang, et al. (2025) under CC BY license. Both...doi.org/10.1101/2024.12.16.628673 Zhou, S., Zhu, Q., Eom, M., Fang, S., et al. (2025). A sensitive soma-localized...Frugal CRISPR kit for classrooms Cell-surface proteomics for neurons and astrocytes By Mike Lacy Cell-...Khakh and colleagues mapped their cell-surface proteomes (Wu et al., 2025). The team expressed extracellularly-anchored...proteins on each cell type, the cell-surface shared proteome of astrocytes and neurons (CS SPAN) featured extracellular... Wu, L., et al. (2025) The cell-surface shared proteome of astrocytes and neurons and the molecular foundations...

  4. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    ...insert a resistance gene. The vector contains a neomycin resistance gene (NeoR) flanked by homology arms...screen for cells where recombination has occurred. Neomycin, puromycin, and hygromycin resistance genes are...remove a resistance gene. In this construct, the neomycin resistance gene is floxed so that it can be removed...the sequence of GFP is cloned together with the Neomycin resistance (NeoR) gene between exon 1 and 3 of...

  6. dTAG - You're it!

    Type
    Blog Post
    ...selective removal of proteins-of-interest from the proteome. Rather than inhibiting protein function, these...Immunoblotting is recommended, while quantitative proteomics experiments will provide the best confirmation...and genetic rescue experiments (Nabet et al.), proteomics (Nabet et al., Erb et al., Huang et al.), transcriptional...

  7. CRISPR Between the Genes: How to Experiment with Enhancers and Epigenomics

    Type
    Blog Post
    ...the genome. Aneesh's research interests include proteomics, machine learning, and visualization for big ...proteins,” ChIP-seq from Table 1. Because of DNA’s 3D geometry and the chemical properties of modified histones...Aneesh's research interests span machine learning, proteomics, and user interfaces for math.     Additional...

  8. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ... Genesis 2014 Jan;52(1):39-48. APEX2 for proteomic mapping and electron microscopy A thorough understanding...microscopy, and as a targeted tool for spatial proteomic mapping in living cells. Despite these advances...APEX for both EM imaging and proximity-dependent proteomic mapping with far greater sensitivity at lower ... subcellular regions or protein complexes for proteomic mapping in live cells. Lam et al., Nat Methods...

  9. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    ... of which are commercially available. Various proteomic and transcriptomic datasets can be used to select...even more challenging. Correlation with RNA or Proteomic data from multiple cell lines/ types with different... antibody-independent methods, like RNAseq or proteomics, it is possible to compare antibody labeling ...

  11. Plasmids 101: Mammalian Vectors

    Type
    Blog Post
    ...do not confuse this with G418 (aka Geneticin). Neomycin should not be used for mammalian expression – ...confers G418 resistance; however, like gentamicin, neomycin is typically used to suppress bacterial growth...

  12. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...organization within the nucleus, also called the 4D nucleome. Visualizing specific loci has historically been...loci, a major benchmark in characterizing the 4D nucleome. CRISPRainbow: Scaling up to 6 colors + white ...

  14. Hot Plasmids - November 2023

    Type
    Blog Post
    ...can identify protein interactions or map a local proteome, but some applications have been challenging or...Cheah, J.S., et al. (2023). Dynamic mapping of proteome trafficking within and between living cells by...

  15. Plasmids 101: Biotinylation

    Type
    Blog Post
    ...organisms have less than five enzymes in the entire proteome that are biotinylated. In E.coli for example, ...biotinylates only one lysine in the entire E.coli proteome. Using this knowledge, scientists identified the...

  16. Using Phosphoserine to Study Protein Phosphorylation

    Type
    Blog Post
    ...staggering ~250,000 phosphorylation events in the proteomes of higher mammals (2). How can we begin to understand...despite abundant incorporation of cysteine in their proteomes (3). This led to the eventual discovery of a naturally...

  17. Antibodies 101: Validation

    Type
    Blog Post
    ...methods may include analysis of transcriptomic or proteomic data from several different tissues or cell types...strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets already exist.   Example...

  18. Rinehart Lab Phosphoprotein Reagents

    Type
    Collection
    ...positions for testing phosphoproteomics methods, proteomics search algorithms, proteomics phosphosite localization...multi-purpose, regenerable, proteome-scale, human phosphoserine resource for phosphoproteomics . Nature Methods ...(V70) (DOCX, 551 KB) . The synthetic human phosphoproteome The Rinehart lab synthesized 110,139 unique...anticipated to be useful for laboratories performing phosphoproteomic experiments in need of reference spectra or...(2022) is adapted from the synthetic human phosphoproteome. It subdivides the 110,139 human phosphoserine...Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent... J. (2018). Kinase substrate profiling using a proteome-wide Serine-Oriented human Peptide library . Biochemistry...

  21. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    ...available as a standard entry clone. ORFs from an ORFeome Gateway library or any PCR-amplified ORF cloned... reporter gene (EGFP, iRFP, IFP1.4, puromycin, neomycin or luciferase) to create a final lentiviral vector...

  25. Hot Plasmids - May 2022

    Type
    Blog Post
    ...Proximity-dependent labeling has become a powerful approach for proteomic mapping in both cell culture and whole organisms...

  29. Working with Nuclear Receptors

    Type
    Blog Post
    .... Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa cells was the...

  37. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    ...antibiotics also block protein synthesis, and the neomycin resistance gene protects against any of them. ...

  40. Evolution of Lab Techniques

    Type
    Blog Post
    ...fourth-generation DNA sequencing technology." Genomics, proteomics & bioinformatics 13.1 (2015): 4-16. PubMed PMID...

  44. Plant Plasmids and Resources

    Type
    Collection
    ...Maize Transcription Factor ORFeome - John Gray. Rice Transcription Factor ORFeome - John Gray. Plant transcription...

  45. Lentivirus Plasmids

    Type
    Collection
    ...with puromycin selection. See plasmid 21916 for neomycin selection. Dmitri Wiederschain 11578 pSico 3rd...Tet-pLKO-neo 3rd Inducible expression of shRNA with neomycin selection. See plasmid 21915 for puromycin selection...

  46. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...Organism Representative Empty Backbones Neomycin (G418) Mammalian, Varies Tet-pLKO-neo - Tet-inducible...selection Find nematode transgenesis plasmids with Neomycin selection Puromycin Mammalian pcDNA3.1-(-)-Pur...

  47. Antibody Guide

    Type
    Collection
    ...done using methods such as transcriptomics or proteomics. This approach is often appropriate for quantitative...validation This method uses peptide identification by proteomics (Capture MS) to validate the identity of proteins...

  48. Resolute Plasmid Collection

    Type
    Collection
    ...core of the deorphanizing engine. Genetic and proteomic interactions represent the framework that provides...

  49. SARS-CoV-2 Pseudotyped Virus

    Type
    Collection
    ...reporter vector expressing firefly luciferase with neomycin resistance. Christopher Vakoc 138152 pLentiEGFPdestablized...

  50. Retrovirus Plasmids

    Type
    Collection
    ... for puromycin selection, and plasmid 1774 for neomycin selection. Bob Weinberg 10676 pMKO.1 GFP MoMLV...
Showing: 1 - 50 of 57 results