We narrowed to 162 results for: Cre

...tissue-specific cell types: Cre-lox recombination. What is Cre-lox? The Cre-lox system is a technology ...marker, Cre can be used to easily perform this eviction. Regulated Cre expression – placing Cre downstream...constructed. Additionally, Cre-containing adenovirus (Ad-Cre) or AAV (AAV-pgk-Cre) has been used to successfully...generated at the loxP sites. How can I use Cre-lox? The Cre/lox system is a well-established research ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...development, or by making the Cre inducible (such as with tamoxifen or doxycycline), the Cre recombinase can be ...facilitate the use of Cre-lox technology, transgenic mice have been constructed that express Cre under a variety...

...be to inject your Cre-dependent mCherry mouse with the pmSyn-EBFP-Cre AAV. Because Cre expression is controlled...We began distributing ready-to-use virus preps through our viral service in late 2016 and requests are...encoding Cre recombinase under the control of the synapsin promoter. It can be used to drive Cre expression... could breed your Cre-dependent mCherry line with a separate line that expresses Cre in neurons, but both...expression of any Cre-dependent gene of interest in neurons. This ability to be paired with any Cre-controlled...interest makes pmSyn-BFP-Cre highly modular. Further uses of pmSyn1-EBFP-Cre Of course there are also ...AAV that became available in 2017 is pmSyn1-EBFP-Cre from Hongkui Zeng’s lab. This AAV has had over 150...

...This post was contributed by guest blogger Katrin Michel. Cre-lox is an incredibly popular and powerful...control without modification - either Cre is there or it’s not. Cre-mediated possibilities for site specific...control FLP and Cre expression have been developed. Read on to learn more about FLP-FRT, Cre-lox, and how...the control of Cre recombinase are used in combination with mouse lines that express Cre only in the cell...LDB) to the C-terminus of FLP or Cre (pCAG-CreERT2 #14797). These Cre/ FLP versions are retained in the...Tamoxifen inducible Cre versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible...about Cre-Lox Check out Retrograde AAVs for Neurscience Research Resources on Addgene.org Find Cre-Lox...

... how Cre-lox recombination works. Advances in Cre-lox recombination have included inducible Cre, such...transgenes and Cre, Cai et al. also created an Autobow construct containing both Cre and XFPs. Cre production...continued utility of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick...membrane tethered cyan). Without Cre recombination, RFP is expressed. Cre can mediate one of two deletions...construct, OFP (orange), is expressed when Cre is absent; stochastic Cre excision results in one of three recombinations...system employs Cre-mediated deletion, the design of Brainbow-2.1 cleverly combines Cre-mediated deletion...remaining cassette can invert as long as Cre remains expressed; once Cre is removed from the system, the fluorescent...

...Plasmid Collections Recombinases Cre-Lox and Other Site-Specific Recombinases Cre Plasmids Flp Plasmids Dre ...prevent gene expression in the absence of Cre. In the presence of Cre, the stop codon is excised, and gene ...until Cre is present, at which time the gene will be disrupted or deleted. shRNA Expression: Cre-lox can...interest, Genes A and B. When Cre is absent, only Gene A is translated correctly. Cre expression excises Gene...double-crossover event. Toxicity Cre-lox recombination is a very useful tool, but Cre expression can be toxic ... such as Cre-only lines to account for cellular toxicity in your system. Using inducible Cre systems in...expressing Cre and other site-specific recombinases. Many of our in-stock viral preps feature Cre-dependent...

...Cre-lox recombination is an incredibly useful molecular biology tool, but like any biological system,...efficiency of Cre recombination varies for different constructs and cell types. Second, Cre may induce recombination...expression of Cre from the standard UAS/GAL4 system is toxic to proliferating cells. A Cre-estrogen receptor... developmental aberrations. In multiple systems, Cre itself, without the presence of a floxed construct...contrast, Dre recombinase, the close relative of Cre, displayed an excision efficiency of only 70%. To...nontoxic. In contrast, 0/79 offspring carried both Cre and GAL4, further emphasizing the toxicity of this...more information about limitations and caveats of Cre usage: Vagaries of conditional gene targeting. Schmidt-Supprian...

... with cell-type-specific Cre, the polyA sequence is inverted only in the Cre-expressing tissues. This ...! What is the CREATE method?  CREATE stands for Cre Recombinase-based AAV Targeted Evolution. In this...using this library to infect an animal expressing Cre in a cell type of interest, a scientist can isolate...28671695. 2. Deverman BE, Pravdo PL, Simpson BP, et al. Cre-dependent selection yields AAV variants for widespread...

...At Addgene, we love GFP, and we’re always excited when depositors find new ways to make this workhorse...transcription or Cre recombinase activity only in the presence of GFP. These systems, known as T-DDOG and Cre-DOG,...Podcast Interview with Connie Cepko Combining Cre and GFP with Cre-DOGs The T-DDOG results showed that GFP ... of interest blocks transcription when Cre is absent. When Cre is present, it removes the STOP cassette...Although Cre isn’t a modular protein, Tang et. al were able to create a split version of Cre that is only...when dimerized by the GBPs/GFP. The new system, CRE-DOG (Cre Dependent On GFP), is activated by GFP and derivatives... cord. Cre-DOG is also suitable for optogenetic studies, and since cells infected with AAV-Cre-DOG retain...

...SSRs) Cre and FLP. SSRs bind DNA at target sites to induce site specific recombination events: Cre recombinase...express GeneR only in the presence of Cre. In this example, Cre would first invert the reporter and GeneR...a cell or tissue-specific Cre is provided. Since the FLEx switch ensures Cre-dependent reporter expression...orientation in the presence of Cre. A similar strategy can be employed for "Cre-off" (i.e. your gene of interest...in the sense orientation and Cre is used to flip it to "off"). These Cre-off switches may be referred ...from our Plasmids 101 series, we learned how the Cre-loxP recombination system can be used to induce site-specific...orientation of the flanking loxP sites directs the Cre recombinase to invert, translocate, or excise a DNA...

.... In tamoxifen-inducible Cre (Cre-ER) systems, Cre is fused to the ligand binding domain of estrogen receptor...the Esr1 domain that blocks Cre expression. Then, Cre is expressed and Cre-mediated recombination of floxed...that regulates Cre expression. The most common drug used to induce the expression of Cre is tamoxifen. ...doxycycline mediated activation of Cre to either induce or repress Cre expression using TetON or TetOFF ...using Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more about Cre-lox ...mice, conditional knockout/knockin mice, use the Cre-lox recombination system to express the transgene...transgene in specific cells or under certain stimuli. Cre recombinase catalyzes the recombination of DNA sequences...

...heterozygous for each mutated allele.  Cre-lox expressing strains Cre-lox strains are a common example of... expressing both Cre and LoxP occurs. But how are these mice made to begin with? Cre-lox mice are made...strain that expresses Cre with a strain that expresses a floxed gene of interest. Cre may be expressed globally...using Cre-lox Read our genome engineering blog posts Resources on Addgene.org Find more about Cre-lox ...to cross mice to create double knockout lines and Cre-lox lines, and how to properly control for genetically...carriers at those alleles. Some alleles, such as Cre, function when hemizygous, meaning that only one ... be either hemizygous or null at one locus (e.g. Cre) and heterozygous at the other. 3. Breed your F1 ...

...combines the Cre/lox system with optogenetics. Cre activity is controlled by red light by splitting Cre and fusing...reduces toxicity as compared to previous Cre/lox or drug-induced Cre systems (ex: tamoxifen). As the name ... dimerize, bringing each terminus of Cre together to restore Cre activity. As soon as red light or PCB...proteins separate, inactivating Cre. This is critical for reducing Cre toxicity in long-term experiments... Figure 4: The C-terminus of Cre (CreC) and the N-terminus of Cre (CreN) are fused to the truncated... and PIF6APB binding, bringing the two halves of Cre together. Image from Eisenhoffer et al., 2019. ...express various fluorescent proteins controlled by Cre recombination. Each cell can have multiple copies...

...Mary Dunlop’s lab generated a photosensitive Cre, Opto-Cre-Vvd, by splitting and joining the protein regions...C-terminal and N-terminal Cre fragments together and permitting recombinase activity. Opto-Cre-Vvd is precise, ...light. Unlike other split-Cre systems which can take up to 24 hours to cut, Opto-Cre-Vvd is fast and can cut...pAAV-hSyn-Cre-P2A-dTomato is now available in AAV1 and AAV8, as well as AAV5 and AAVrg. Find the Cre-expressing... you'll find in this post: Blue-light inducible Cre for use in bacteria CRISPR-Casɸ mGold, a new yellow...the viral service A fast blue-light inducible Cre for precise control of bacterial gene expression ...recombinase activity were exposed to blue light. Cre-Vvd showed substantially improved activity as compared...

...fluorescent protein genes, Cre recombination is required before fluorescence can occur. Cre recombinase can be...developing fish via Cre protein microinjection or crossing of Zebrabow animals to an inducible Cre line. The Schier...OFP (orange) (Livet et al., 2007). Brainbow uses a Cre/lox system to label neurons. Genes encoding the fluorescent...gene directly following the promoter is expressed. Cre-mediated deletion of one or several of these genes...system. One such system is Brainbow 2.0, which uses Cre to trigger the inversion of two genes aligned head-to-head...head-to-head. Another variation, Brainbow 2.1, uses Cre to trigger the inversion of two tandem sets of genes...when crossed with mice expressing the appropriate Cre allele. The Confetti mouse contains the Brainbow ...

...dependent Cre. The two segments come together in the presence of GFP to form a functional Cre enzyme that...ChR2 for targeted expression DREADDs GFP dependent Cre and FLPo And more!  New lentiviral preps SARS-...control plasmids in new serotypes, as well as a new Cre dependent GFP control plasmid for expression in forebrain...future therapies. Our popular plasmid encoding a Cre dependent version of the DREADD hM3D is now also ...Roth   Recombinases Recombinase systems such as Cre-lox allow for more accurate cell and tissue targeting...recombinases from the Cepko Lab are now available as AAV1. CRE-DOG constructs 69570 and 69571 together encode the...restrict gene activation to cells expressing both Cre and GFP. The GFP dependent FLPo, Flp-DOG (75469),...

...cells with an inactive opsin gene; in Cre expressing cells, however, Cre induces the recombination of the ...achieved through site-specific recombinases such as Cre or Flp that induce DNA recombination at defined recognition...loxP and lox511) in opposite orientations. Since Cre does not cause recombination between mismatched recognition...specific neuronal cells? This can be achieved using Cre-dependent viruses, such as the optogenetic FLEx vectors...combination with transgenic animals or rAAVs expressing Cre under a specific cell-type promoter. Once injected...behind the mechanisms of sleep and anesthesia. In Cre transgenic mice, they targeted the dopamine (DA) ...of FLEx vectors combined with the use of several Cre transgenic mice targeting different brain areas enabled...

...Flp-dependent Cre AAV in AAV9 and AAVrg. See our new additions here: Cre vectors pAAV-EF1a-fDIO-Cre (121675...expression. This is a Cre-dependent switch that expresses nuclear mCherry in the absence of Cre, and expresses...expresses nuclear EGFP in the presence of Cre. Biosensor AAV (calcium sensors and GABA sensors) Calcium sensors...sensor (see below), in addition to our inventory of Cre-dependent GABA sensors. See all our GABA sensor AAV...

... Mammalian shRNA Tools for RNAi! Cre-Lox technology CAG-GFP-IRES-CRE (Plasmid #48201) This retroviral ...vector from the Gage lab encodes GFP and Cre-recombinase. The Cre-lox system is a technology that can be...of mouse transgenics. Experimental uses include: Cre-dependent gene expression, gene knockout, and selection... how to use the pLKO.1 vector can be found here. Cre-Lox technology Puro.Cre empty vector (Plasmid #17408... vector deposited by Dr. Tyler Jacks. It carries CRE recombinase and a puromycin resistance cassette. ...shRNA), mutation/editing (CRISPR-Cas9) or deletion (Cre), together with expression of fluorescent or enzymatic...

...Constitutive or inducible Cre recombinases can be introduced by AAV or by mating with Cre-expressing mice. ...the Cre-dependent SPH construct for generating the SPH mouse. (B) AAV plasmids used to express CRE under... of Cre-dependent mouse lines for overexpressing genes within discrete cell populations makes Cre-dependent...importantly, the expression of this construct is Cre-dependent, enabling spatiotemporal control over its..., how many sgRNA expression cassettes will fit). Cre-dependent expression of the SPH activator paired ...populations.  The Yang Lab’s AAV plasmids used to express Cre under neuron-specific promoters, including calmodulin-dependent...