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We narrowed to 138 results for: THY-2

Showing: 91 - 100 of 138 results

  1. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    ...’re delivering their payload to.   Figure 2: Numbers of AAV tools, by category, available from... substrates, light, or engineered ligands (Figure 2). Biosensors, which include calcium and voltage sensors...significant time and removes a variable from the lengthy process of validating and using an AAV in a specific...

  2. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...the chromosome, ending replication.   Figure 2: Prokaryotic chromosomal replication. Adapted from...Microbiology and Molecular Biology Reviews: MMBR, 62(2), 434–464. https://doi.org/10.1128/MMBR.62.2.434-464.1998...contribute to this regulation in different ways: methylating newly synthesized DNA, blocking binding sites...

  3. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl sulfoxide-mediated primer...isolated from a methylation competent bacterial strain (e.g. DH5α which is dam+). Methylation deficient bacterial...template. The parent template is removed using a methylation-dependent endonuclease (i.e. DpnI), and bacteria... of the primers. Because you will be using a methylation-dependent enzyme (DpnI) to eliminate the parent...is unique in that it cleaves only DNA that is methylated at the adenosine of the GATC recognition site...

  4. Plasmids 101: E. coli Strains for Protein Expression

    Type
    Blog Post
    ...restrict methylated DNA fhuA2 lacZ::T7 gene1 [lon] ompT gal sulA11 R(mcr-73::miniTn10--TetS)2 [dcm] R... restriction/methylation system. This means the strain can neither restrict nor methylate DNA. dcm: Similarly...Similarly, strains with this mutation are unable to methylate cytosine within a particular sequence. Browse... in the exponential growth phase are alive and healthy, which makes them ideal for protein expression....

  5. Empty Backbones - Choosing Your Perfect Plasmid Backbone

    Type
    Collection
    ...backbone with MS2 loops at tetraloop and stemloop 2 and EF1a-zeo resistance marker pLenti CMV/TO GFP-Zeo... for gene expression Thy1.1 Mammalian pLKO.3 Thy1.1 - Lentiviral shRNA expression Gentamicin...

  6. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...hypoxanthine) or between the pyrimidine bases (cytosine, thymine, and uracil). Base transversions are edits that...have an enzyme to remove inosine from DNA: N-methylpurine DNA glycosylase (MPG), also known as alkyladenine...BioRender.com. Base excision editing extends to thymine Since an enzyme that excises inosine can be modified...which excises uracil, could allow it to recognize thymine (T) or cytosine (C). Several different teams succeeded... DNA, creating an abasic site. MPG / AAG N-methylpurine DNA glycosylase / alkyladenine DNA glycosylase...editing by fusing an adenine base editor with an N-methylpurine DNA glycosylase. Nature Biotechnology, 41(8)...

  7. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ... from damage (Abe & Lim, 2024).    Figure 2: A) When proteins (blue) interact with the air-water...editor consisting of dCas9 fused to a DNA methyltransferase — that can drive lasting transcriptional silencing...Histone tail for Autoinhibition Release of Methyltransferase (CHARM), with several advantages over the ...  Rather than overexpressing an exogenous methyltransferase for epigenetic silencing, which can be toxic...tail to recruit and stimulate the endogenous methyltransferase. The strategy is generalizable to a variety...D3L recruits the endogenous, autoinhibited methyltransferase DNMT3A to the genomic location specified by...DNA-binding protein. The ADD domain of the methyltransferase binds to H3K4me0 on D3L, releasing the autoinhibition...

  8. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...posts: Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse...is less predictable. In our best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA...:1262–1267 . https://doi.org/10.1038/nbt.3026 Gurumurthy CB, Takahashi G, Wada K, Miura H, Sato M, Ohtsuka...

  9. CRISPR Guide

    Type
    Guide
    ...left free to interact with the target DNA. Figure 2: Overview of the NHEJ repair mechanism Cas9 will only...systems enable researchers to target anywhere from 2–7 genetic loci by cloning multiple gRNAs into a single...Cas9 is included in the gRNA-containing plasmid, or 2-vector systems, in which Cas9 must be delivered separately...your experimental cell population (Figure 8E). In a 2-vector system, you’ll need to either co-infect with...the presence of infectious organisms (like SARS-CoV-2 ) and genetic mutations. Similar to Cas9 and Cas12...Cas enzymes? CasPEDIA is an encyclopedia of Class 2 CRISPR systems with wiki entries describing enzyme...off-target effects by using a single Cas9 nickase and 2 different gRNAs, which bind in close proximity on ...

  10. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...as well. What I really brought to the table was empathy, a willingness to listen, and an ability to remember... are a few more tools that can come in handy: Empathy: If you often find yourself in confrontational ...critical component of conflict resolution. Having empathy does not mean you like everyone you meet. Most ...solid examples of times when you have used your empathy, listening skills, and institutional knowledge ...
Showing: 91 - 100 of 138 results