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We narrowed to 399 results for: MUT

Showing: 181 - 200 of 399 results

  2. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type
    Blog Post
    ... HEPN domains in other proteins. As with Cas9, mutating key residues in the Cas13a molecule results in...Zika virus, genotype human DNA, identify tumor mutations within cell-free genomic DNA, and detect COVID...

  3. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    ...biosensors are generated by inserting a circularly-permuted GFP into an intracellular loop of various G-protein-coupled...around 70 mM and 100 mM, respectively. Introducing mutations and using potassium-binding protein homologues...

  4. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    ...2013) Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas...Rice Blast Resistance by CRISPR/Cas9-Targeted Mutagenesis of the ERF Transcription Factor Gene OsERF922...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...mEGFP. This variant was made by introducing two mutations (F64L, S65T) in the original jellyfish GFP (AvGFP...with improved brightness (Tsien, 1998). Another mutation (A206K) is necessary to generate the strictly ...

  7. CRISPR 101: Epigenetics and Editing the Epigenome

    Type
    Blog Post
    ... spiroplasma (M. Sss1) (termed MQ1.) The Q147L mutation improves methylation kinetics such that cytosine...fused LSD1 to x-Cas9(3.7). x-Cas9(3.7) is a SpCas9 mutant that exhibits increased PAM flexibility, and this...

  8. Live and Let Dye: Self-Labeling Protein Tags

    Type
    Blog Post
    ... most often consist of an enzyme that has been mutated to block its catalytic activity and instead become... later work showed that introducing a Leu28Cys mutation in eDHFR does lead to covalent binding of the ...

  9. Quick Guide to All Things Lentivirus

    Type
    Blog Post
    ...active area of research due to the possibility that mutation or recombination with human retroviruses could...Efficient gene transfer Potential for insertional mutagenesis: Replication-incompetent lentiviruses with human...

  10. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    ...with senior investigators. Discussions can reveal mutual scientific interests, complementary perspectives...research to search committees, and can lead to mutually beneficial collaborations. 7. You cannot get too...

  11. Important Considerations When Using AAVs

    Type
    Blog Post
    ...them. There are suspected cases of insertional mutagenesis in humans (1) and AAVs are non-enveloped viruses... Russell, David W. "AAV vectors, insertional mutagenesis, and cancer." Molecular Therapy 15.10 (2007):...

  15. CRISPR Cheat Sheet

    Type
    Blog Post
    ...multiple genes in a cell population. The resulting mutant cells are screened for phenotypes of interest. ...

  18. New Viral Vectors - Summer 2024

    Type
    Blog Post
    ...viral service with multiple serotypes pAAV-hSyn-GRAB_OTmut AAV9 Biosensors (Control) Li New viral service...

  19. What's New in CRISPR - May 2019

    Type
    Blog Post
    .... Thus, they engineered two CBE variants with mutations in the rat APOBEC1 that decreases the number of...
Showing: 181 - 200 of 399 results