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We narrowed to 604 results for: abo.2

Showing: 361 - 400 of 604 results

  1. March for Science

    Type
    Blog Post
    ...citizens enter into in order to protect their rights (2). Less formally, governments are the organizations...science education and literacy that this changes.  2) Lumping all scientists (and consequently their results... with a testable hypothesis - an educated guess about how some part of the world functions. Experiments...governments exist in reality and science is how we learn about reality. Formally, a government can be defined ... More concretely, scientists gather information about reality informing people and their governments on...people to rally for science, others are concerned about possible unintended consequences of a march. Three...Democrats more often than Republicans, the March is not about Democrats or Republicans (16). This is a Pro-Science...

  2. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...similar optical quality (magnification of 140X and 2 micron resolution). I have worked many hours in a ...When you think about going to a scientific conference, you may think about sitting amongst a sea of chairs...After learning about using sound to visualize bacteria in the lab, I was excited to learn about creative approaches... at different ultrasound frequencies. Read more about ‘Using ultrasound to image bacteria in vivo: acoustic...Protein Resources and get started!  I learned a lot about creative ways to do science communication and outreach...outreach - if you'd like to learn more about Science communication check out these SciComm blog posts.... Methods for Bacterial Genome Engineering Learn about Science Communication using Twitter  Resources ...

  3. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

    Type
    Blog Post
    ...develop therapies to treat disease in the lung. Chapter 2: Balancing research with medical practice as an MD...promote scientific sharing. Listen to learn more about Darrell, his research, and his focus on open science...Medicine and in the Department of Pathology and Laboratory Medicine. He attends in the Medical Intensive...NIH-funded Principle Investigator in the Kotton Laboratory and is the founding Director of the Center for...Podcast.     Additional Resources from Addgene Learn about Retinal Gene Therapy from Connie Cepko Find Plasmids...Plasmids from the Kotton Lab Learn more about Stem Cells ...

  5. A Conference By Postdocs For Postdocs: Future of Research

    Type
    Blog Post
    ... on the future of the scientific endeavor October 2-3, 2014 Boston University Jacob Sleeper Auditorium...doing science in some form? There is a lot of talk about the state of scientist training in the US and around...

  7. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

    Type
    Blog Post
    ...Transgenesis." PLoS ONE. 8(12): e83043 (20 December 2013). 2. Carola Engler et al. "A One Pot, One Step, Precision...protein,” he says. “There is nothing you can do about it but be gentle. If it no longer works, just trash...

  8. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    ...phosphoinositides (Varnai and Balla, 2008) (Figure 2). RNA production can be detected by using a MS2 coat... 296.5569 (2002): 913-916. PubMed PMID: 11988576. 2. Tsien, Roger Y. "The green fluorescent protein." ...deleting residues. Only about 10 amino acids can be deleted from the C-terminus and about 5 from the N-terminus...cylinder with a length of 4.2 nm and a diameter of about 2.4 nm (Hink et al., 2000). The complete beta-barrel...based on cofactor binding proteins as described above. The smallest tags are peptides that are engineered...

  9. Top 10 Open Science Developments of 2013

    Type
    Blog Post
    ...access to the results of federally-funded research. 2)      In June, the White House honored 13 “Champions...study probably doesn’t say as much about open access as it does about the state of peer review. 8)     ...University Open Data Access (YODA) Project, “a collaborative endeavor guided by aspirations to promote open...

  10. Antibodies 101: Beyond Surface Labeling

    Type
    Blog Post
    ...would label everything but the CD8+ T cells (Figure 2). Even better, we can use the same fluorophore for...gating on the negative population.     Figure 2: Dump gating labels all unwanted cell lineages with..., you may want to look at other methods. In the above cases, a good alternative is using a two-protein... region of the primary antibody (Figure 1b). As above, the advantage of this system is increased flexibility...

  11. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...to a specific area at the plasma membrane (Figure 2). Just express the plasma membrane-localized iLID ...cells will be ready for the spotlight.    Figure 2: Opto-RhoGEFs to control Rho GTPase activity. A) Schematic... Check out our Optogenetics Guide to learn more about how these modules work, and read on to learn how...to the cell membrane isn’t powerful enough, what about altering the chemical composition of the membrane...

  12. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...wheat germ, HeLa, and Leishmania (Gagoski, et al 2015[2]). The proteins encoded in this set range in size ...to generate Illumina sequencing libraries using a 2-step PCR-based protocol and are compatible with single-end...PUF Assembly Kit Below you’ll find information about what these kits contain, how they can be used, and...encoded sensors enable real-time observation of metabolite production." Proceedings of the National Academy...

  13. Our Most Popular Blog Posts from 2019

    Type
    Blog Post
    ...fusion location, there are many choices to make. 2. Prime Editing: Adding Precision and Flexibility to...mNeonGreen, mTFP1, and mWasabi by Jennifer Tsang Learn about mNeonGreen, mTFP1, and mWasabi and find plasmids...Broccoli, Spinach, and Corn, for example. Learn more about the aptamer soup in this blog post. 5. Five Popular...

  14. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    ... a template, copying the base edit. Figure 2: Cytidine deamination takes place on the free strand...SpCas9 base editors with editing windows as small as 1-2 nucleotides. To reduce off-target effects associated...formation. To further decrease indel formation 1.5-2 fold, the team fused bacteriophage protein Gam to ...Homology-Directed Repair Learn about base editing for plants Read about four base editor reporters Additional... The BE3 system increased editing frequency to above 30% for a variety of targets in human cell lines... Liu lab created BE4max and AncBE4max mentioned above, they also made an adenine base editor with improved...cell therapy applications. Dual base editors What about combining the function of both editors into one?...

  15. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    ...Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker (Ginkgo Bioworks)...their curation of citable, linkable figures. Cause #2: Hidden “negative” data We all want to be able to ...easier to find information about research tools. BenchSci collates information about antibodies while FPbase...variety of additional resources and information about organizations promoting reproducibility in this ...that make it easier to share in-depth information about reagents, protocols, analysis tools, and more. Being...from a freezer. As you’ll see in Steve Almo’s talk about work at the Institute for Protein Innovation (IPI... talked to many researchers who have complaints about the unreliability of antibodies. IPI is working ...

  16. Finding Your Science Policy Path

    Type
    Blog Post
    ...teaching and mentoring others (see more in Figure 2). Starting early demonstrates not only your interest...application process so you can learn more! Figure 2: The many ways to prepare for a science policy career...learn more about their career trajectory. If there’s one thing everyone loves, it’s talking about themselves...learn more about the field.  Read the CCST Science Policy Career Guide to learn more about science policy...Administration, I could never get one straight answer about what science policy even is! In this article, I ... the #scipol hashtag. Often there will be posts about virtual meet ups and happy hours that you can attend...solving one problem. It often requires thinking about a problem in a new way through the integration of...

  17. From our Table to Yours: An Inside Look at Lunch at Addgene

    Type
    Blog Post
    ...and trophies are awarded and coveted. I’ve only won 2 trophies over the years, which is either an indication...for the company, but also talking about sports, movies, and just about anything. As our organization grew... with our western blots. Thoughts about sharing Addgene is all about helping scientists share between ...culture of openness and trust that comes from caring about your colleagues on a personal level, and employees...often cite lunch as one of their favorite parts about working at Addgene. We’re a small non-profit, so...indication of the stiff competition or says something about my cooking.   This is a creative (and delicious)...for people in different labs to chat informally about their challenges, successes, and expertise. Who ...

  18. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    ...posts: Part 1: Genetically Engineered Mice and Part 2: Breeding and Crossing Mice. My first CRISPR mouse...is less predictable. In our best case, we saw that 2 out of 3 mice carried a 5 kb insertion in the ROSA... Delivery Methods Learn about Mouse Work on Exercise and Depression Read About Brainbow Resources on ...success is not always guaranteed. To learn more about this process check out our Mouse Modeling blog posts...by CRISPR. The sequence started clean and then, about 100 bps into the run, it got “dirty”, with multiple...makes use of 3 components, a guide RNA (gRNA) of about 125 nt that specifies the target, the Cas9 endonuclease...by Southern blot. From the efficiency discussed above extends 4 major advantages of CRISPR mouse editing...

  19. Three Tips to Organize Your Lab Notebooks in the New Year

    Type
    Blog Post
    ...that you can easily spot the colors and text. Tip 2: Use electronic to-do lists to access your tasks on...experiments before the next R01 is due. But what about organizing yourself and your lab? Organizing your...

  20. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

    Type
    Blog Post
    ...bringing in environmental contaminants.  Figure 2: An Addgenie works in the tissue culture hood while...doi.org/10.1290/1071-2690(2002)038<0086:caeomi>2.0.co;2 Uphoff CC, Drexler HG (2011) Detecting Mycoplasma ...mycoplasma contamination in your cell culture or you are about to begin a new cell culture project. If mycoplasmas...that are difficult to trace. These include the laboratory personnel, the serum, the cell culture media,...accounts for the largest source among those mentioned above. The contaminants can spread through dirty clothing...proliferating shortly. Once they are present in the laboratory environment, they are difficult to eradicate....

  21. Antibodies 101: Validation

    Type
    Blog Post
    ...another. You’ll need to validate that points 1 and 2 hold true under your specific experimental conditions...when (1) other strategies may not be feasible and (2) large-scale proteomic and transcriptomic datasets...may not be the most accessible option for many laboratories. Additionally, it can be difficult to distinguish...the captured proteins off for mass spec analysis (above). When the results come back it shows that the majority...our Selecting an Antibody blog post! Learn more about Antibodies and their many applications in our Antibodies...

  22. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    ... a western, proteins are: (1) separated by size, (2) transferred to a membrane, and  (3) detected using...expensive system-specific sandwiches.   Figure 2: The western blot dry apparatus includes a stack of... the thickness of the band provides information about the relative abundance of the protein in the sample...history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using...

  23. Six Spooky Science Stories and Halloween at Addgene

    Type
    Blog Post
    ...melanogaster." Molecular and cellular endocrinology 215.1-2 (2004): 1-10. PubMed PMID: 15026169. Soo, Rochelle... Additional resources on the Addgene blog Read about company culture at Addgene See some of our previous...

  24. 6 Steps to Submitting a Resume That Gets Seen

    Type
    Blog Post
    ...having trouble preparing your most excellent resume. 2) Follow job boards and check them regularly Use your... your strengths in your resume and don't be shy about asking for help. The worst thing that can happen...

  25. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    ...), 1668. https://doi.org/10.1038/s41467-019-09656-2 More resources on the Addgene blog CRISPR101: Validating...LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested in...K.B., Gillis, J.E., Sen, D.R., Gaudiano, E.F., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H...

  26. Tag Your Favorite Yeast Genes with Ease

    Type
    Blog Post
    ...Pringle’s former lab at UNC Chapel Hill. Bähler et al.(2) describe a modular collection of plasmids for a wide...strain. In addition to the collections featured above, many other modular yeast tagging systems have been...Addgene would love to hear from you, our community, about your experience with yeast genome modifications....

  27. Cre-ating New Methods for Site-specific Recombination in Drosophila

    Type
    Blog Post
    ...CHO) cells, as well as in cortical neurons in layer 2/3 of the mouse brain. Rubin’s work shows that the ...site-specific recombination in Drosophila, read on to learn about new recombinases suitable for this system. Gerald...recombinases from the Rubin lab For more information about limitations and caveats of Cre usage: Vagaries of...

  28. The Open Repository of CRISPR Screens: CRISPR Screen Data in One Place

    Type
    Blog Post
    ...might otherwise be hard to identify. Figure 2: Users can cross-interrogate screens from the literature...resources on the Addgene blog Read this blog post about genome-wide screening using CRISPR Find all of our...on Addgene.org Read our CRISPR guide Learn more about plasmid pooled libraries Find CRISPR plasmids for...

  29. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    ...six different research constellations: Doench/Root (2), Zhang (3), Wu (4), Yusa (5), Wang/Lander/Sabatini...Bioinformatics 33.7 (2016): 1099-1100. PubMed PMID: 28414855. 2. Doench, John G., et al. "Optimized sgRNA design to... to design gRNAs for all the purposes discussed above. Please see videos and text on the website for more...Output: A text file containing full information about the suggested gRNAs to use, including all information... hard to read. It´s suggested that you open the above text files, copy the content of the file, and paste...Additional Resources on the Addgene Blog Learn about CRISPR Screens How to Design Your gRNA Tips for ...CRISPR User Resources on Addgene.org Learn More About CRISPR Find CRISPR Screening Libraries Browse All...

  30. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    ...closer to the clinic (Tsai and Joung 2016). Table 2: Unbiased Genotyping Options Technique Detection...PMID: 23792628. PubMed Central PMCID: PMC3773023. 2. Vouillot, Léna, Aurore Thélie, and Nicolas Pollet...et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming and cannot be applied to heterogenous...viable options for multiplex sequencing in many laboratories. Nevertheless, unbiased detection isn’t as sensitive...unbiased sequencing may not be worth the cost and labor when single clones are selected. Conversely, translational... Hough is a Biochemistry PhD candidate in the laboratory of Prof Steve Jackson at the Gurdon Institute...

  31. Seeing Red: Simple GFP Photoconversion

    Type
    Blog Post
    ...photoconvertible fluorescent protein. Nat Methods. 2009 Feb 6(2):131-3.  PubMed. Find plasmids from this publication...this protein has a few more secrets - during a collaboration, members of Maureen Hanson’s and Rima Menassa's...

  33. What's the Best Way to Elute and Store Your Plasmid DNA?

    Type
    Blog Post
    ...elution choices: TE, Tris buffer, or water Figure 2: Tris-EDTA, Tris, or water? The buffer you choose ...to purify DNA without a kit Read our blog posts about plasmid cloning Browse our Plasmids 101 blog posts...

  34. Bricking Science: Portraying Scientific Reality Through LEGO

    Type
    Blog Post
    ...motion videos is very time consuming; producing a 1-2 minute video takes an entire week of our free time...finally decide the LEGO laboratory was good enough. There are no pre-made LEGO laboratory sets so we had to ...everything now is about the plot. This part is easy, after all, who working in a laboratory has never faced...dysregulated water bath boiling your experiments, just about anything can disrupt your bench work and sometimes...to show people the very human struggles in the laboratory routine. We hope that sharing and laughing at...can learn with each other through globalized, collaborative thinking. LEGO and science: The potential is...while opening a LEGO set with my husband, I thought about how the limitless design possibilities of the LEGOs...

  35. 10 Ways to Share Your Science!

    Type
    Blog Post
    ...of your science but also something about how science really works. 2)   Read science writing in magazines...take on collaborative roles in academia, and that means it’s important to know how to talk about science...talking about and sharing some science on the one you already have. Read my earlier post about Scientists...important for scientists to get out there and talk about their work. To tell others why that work is cool... than ever before for scientists to communicate about their work and their interest in science more generally...already make it a habit to talk – really talk – about the research you are doing in the lab with your ... told. Twitter can be a good way to follow news about particular subjects or find out what others are ...

  36. Viral Vectors 101: Parts of the AAV Transfer Plasmid

    Type
    Blog Post
    ... temporal activation of a transgene. Figure 2: FLEX Cre-Lox system.  WPRE The Woodchuck Hepatitis...the Addgene blog Learn more about basic viral vector elements Read about four considerations for the ...Key elements of an AAV plasmid First let’s talk about the key parts of an AAV plasmid. Inverted terminal...routine plasmid propagation, with complete ITR loss abolishing AAV packaging. To prevent ITR loss, it’s best... Other cis-regulatory elements Next let’s talk about some of the elements that aren’t required parts ...the perfect AAV tool Learn about synthetic promoter AAVs for cell-type expression in retinal cells Resources...

  37. Extracurricular Activities for a Strong Science Career Path

    Type
    Blog Post
    ... sets Taking the time to identify and research 2 – 3 potential professions will highlight which skills...This great resource allows the undecided to learn about a variety of science professions based on their ...training focuses on using multidisciplinary, collaborative, and translational approaches for scientific...skills. Although the word “extracurricular” may be taboo in the lab, multiple research studies highlight ...social media (Twitter, Facebook, etc.) to learn about available jobs, fields, and trends “Never Leave...from your lab bench.  Want further information about shaping your career to your values, interests, and...Let us know what topic you'd like to learn more about next. Leave your suggestions in the comments section...

  38. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    ... done with CRISPR technology in just 2 years and excitement about how much more there is left to do with...It's been about 14 years since I last attended a Keystone Meeting – far too long. Holding these meetings...downstream output. Tuesday morning's talks were also about circuitry and synthetic biology, and I found myself...plasmid sharing to those who were anxious to hear about what was coming next. I also got to show off our... updated Synbio page to people who were curious about these newest features of our website.  I want to...

  39. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    ...bioengineering 94.2 (2006): 322-329. PubMed PMID: 16528756. 2. Oh, Steve KW, et al. "Substantial overproduction ...resources on Addgene.org Learn about Addgene's viral service Learn about viral production Find viral protocols... By now you have probably heard about Addgene’s viral service but did you know that Addgene also performs... approach can be adapted by vector cores and laboratories alike as an easy and inexpensive method to improve...Effect of medium osmolarity on hybridoma growth, metabolism, and antibody production." Biotechnology and ...

  40. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    ...project my team & I have poured our hearts into for 2 yrs) has 200K+ figures by 95K+ scientists 👨🏽‍🔬👩‍... for the Addgene blog, I was focused on writing about new scientific techniques and cool plasmids. Creating... graphics were usually the last thing I thought about when writing posts. Since then I’ve realized my ...know of a tool or resource not mentioned? Tell us about it in the comments section!  Additional resources...scientists Find science career blog posts Learn about other lab software ...
Showing: 361 - 400 of 604 results