1. AAVs CREATed for Gene Delivery to the CNS and PNS

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2. Science communication: 9 Strategies to Get Your Foot in the Door

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3. Science Careers: Unruly Interests Feed Many Paths

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4. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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5. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

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6. Some Like it Hot: Thermostable GeoCas9

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7. AAV Vector Quality Control: Going the Extra Mile with NGS

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8. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

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9. Cloning Mammalian Cells with the Agarose Method

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10. Pushing the Limits of DNA Assembly

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11. 3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells

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12. Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins

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13. Human Germline Editing Using CRISPR

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14. Fluorescent Proteins 101: History of Fluorescent Proteins

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15. Visualizing Translation at the Single Molecule Level

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16. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

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17. Fluorescent Protein Travel Awards - FLiPs and Fluorescent Protein Biosensors

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18. Lighting Up Cell Signaling with Photoswitchable Kinases

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19. Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS

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20. Supporting Rare Disease Research with BeHeard 2017

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21. Addgene-Seeding Labs Plasmid Grant: Accelerating Science Globally - 5 Scientists, 4 Countries & 3 Continents!

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22. DIY DNA Ladders from Penn State University

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23. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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24. Custom CRISPR Screens & the Green Listed Software

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25. Phage Directory: From Phage Therapy to a Repository of Phage Information

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26. 15 Years of Addgene: The Top 15 Plasmids

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27. CRISPR-mediated Plant Base Editors

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28. The Crispr Babies Saga Shows the Need for Action, Not More Delays

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29. What's New in CRISPR - Winter 2018

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30. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

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31. Early Career Researcher Toolbox: Social Media for Scientists

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32. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

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33. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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34. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

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35. Unique and persistent IDs for improved reproducibility: Addgene now supports RRIDs and compact identifiers for all plasmids

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36. Plasmids 101: Biotinylation

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37. Academic vs. Industry Postdocs

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38. Natural or synthetic? How Addgene’s dataset reveals trends in biological innovation

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39. Starter Guide to induced Pluripotent Stem Cells (iPSCs) Part 2:  Reprogramming and Transdifferentiation

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40. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

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41. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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42. Tips for arabidopsis transformation

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43. Tips for a 1st time AAV user (by a Rookie AAV user)

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44. Simplify Cloning with in vivo Assembly

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45. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

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46. 9 tips for a successful postdoctoral experience

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47. New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries

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48. Is this the right place for me? 8 tactics for choosing a lab

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49. Plasmids 101: Codon usage bias

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50. Celebrating One Million Plasmids Shared!

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