We narrowed to 784 results for: SCR;

1. Inntags: Innovative Protein Epitope Tagging

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   Blog Post

   ...First described in the 1980s, protein tags are now one of the most useful items in a scientist’s toolbox...

2. How Exercise Purges Stress and Keeps Depression at Bay

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   Blog Post

   ...recent issue of Cell demonstrate how PGC-1a1, a transcriptional coactivator induced in skeletal muscle by exercise...

3. Stem Cell Models for Disease & Open Science: Interview with Darrell Kotton

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   Blog Post

   ...University Center for Regenerative Medicine. Darrell describes his career goals and what drives him as a praticing...

4. Your Top Requested Plasmid in 2016!

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   Blog Post

   ... the Zhang Lab's Nature Protocols Publication describing them here. References 1.Ran, F. Ann, et al. ...

5. What's New in CRISPR - Spring 2019

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   Blog Post

   ...Cas12a inhibitors Using a bioinformatics and a screening approach, the Jennifer Doudna lab identified three...

6. CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping

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   Blog Post

   ...cleavage, the authors showed that Cas14 could indiscriminately cleave ssDNA in trans like Cas13 and Cas12...

7. Quick, Versatile Plant Transgenesis with GreenGate Plasmids

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   Blog Post

   ...Introducing GreenGate As Lohmann and his colleagues describe in the PLoS ONE paper that introduced the kit ...

8. Our Most Popular Blog Posts from 2019

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   Blog Post

   ...editing. Using a fusion between Cas9 and reverse transcriptase and a prime editing guide RNA, it can introduce...

9. Top Requested AAV of 2017: pmSyn1-EBFP-CRE

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   Blog Post

   ...Versatile Tool for Gene Expression in Mammals Subscribe to Viral Vector Blog Posts Additional Resources...

10. Bringing Sustainable Practices to the Lab: Innovations

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    Blog Post

    ...Learn about recycling initiatives for the lab Subscribe to the Addgene blog Find lab tips for your research...

11. Editor's Choice, August 2016

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    Blog Post

    ...choice picks this month, I’m picking two posts that describe methods to make single base edits in DNA. The ...

12. Top 10 Open Science Developments of 2013

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    Blog Post

    ...     Science’s John Bohannon published an article describing a sting operation in which he submitted a phony...

13. X-CHIME: Context Dependent Germline Knockout in Immune Cells

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    Blog Post

    ...2019). A CRISPR-Cas9 delivery system for in vivo screening of genes in the immune system. Nature Communications...

14. Phage Directory: From Phage Therapy to a Repository of Phage Information

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    Blog Post

    ...developments at Phage Directory and in the phage field, subscribe to their newsletter, Capsid and Tail. Additional...

15. Searchable and Sortable gRNAs for Your Next CRISPR Experiment

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    Blog Post

    ...RNPs (ribonucleoproteins) and therefore usually transcribe your gRNAs in vitro, you can send us the sequences...

16. Easing the Protein Purification Process with pCri

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    Blog Post

    ...that your protein might not fold well, you should screen a few different vectors to see which gives you ...

17. Science as Art

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    Blog Post

    ...molecules, analyze terabytes of information on black screens, but not to know even the life cycle of the creature...

18. Three Tips to Organize Your Lab Notebooks in the New Year

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    Blog Post

    ...labels. Color code your Post-its and write brief descriptions on each. Place the notes sticking up out of ...

19. A Better Way to Get Customer Support: The Help Center

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    Blog Post

    ...your name and email address, after which you can describe your issue and press send. Doing so will bring...

20. Mapping the 4D nucleome with CRISPR/Cas9

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    Blog Post

    ...many applications, the most well-known being transcriptional activation/repression. dCas9 has also previously...

21. Viral Vectors 101: Parts of the AAV Packaging Plasmid

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    Blog Post

    ...capsid. Rep proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives...

22. Chemogenetics vs. Optogenetics: Which Method Should I Choose?

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    Blog Post

    ...regions of a neuron. Optogenetic approaches excel at discrete cell or tissue-subregion targeting down to the...

23. We're Updating Our Hold for Publication Policy

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    Blog Post

    ... release plasmids on hold if they are clearly described in a journal publication. Plasmids will be released...

24. Antibodies 101: Affinity Tags

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    Blog Post

    ...protein for downstream applications Below, we describe a few of the most common tags available. Maltose...

25. Early Career Researcher Toolbox: Free Online Molecular Biology Tools

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    Blog Post

    ...repository for Cas enzymes.  Open repository of CRISPR screens Cellosaurus: A knowledge resource for cell lines...

26. Antibodies 101: Fc Effector Functions

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    Blog Post

    ... 2022). So the next time you see an antibody description with the Fc domain as only providing structure...

27. Using Ultrasound to Image Bacteria in vivo: Acoustic Reporter Genes

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    Blog Post

    ... and have already developed a high-throughput screening method for serial acoustic collapse to aid in ...

28. CUT&RUN: An Improved Method for Studying Protein-DNA Interactions

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    Blog Post

    ...McKay lab we study how differential access to transcriptional regulatory elements is controlled during development...

29. Oh, The Places You Can Go: Careers in Science Communication - Curriculum Development

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    Blog Post

    ...Hutchinson Cancer Research Center. And make sure to subscribe to the Career Channel of the blog so you don’t...

30. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

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    Blog Post

    ...blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org ...

31. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

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    Blog Post

    ...your science, whether it’s in presentations, manuscripts, or social media. If you want to draw your own...

32. Bringing Sustainable Practices to the Lab: Recycling

    Type

    Blog Post

    ...PMC5517107. Additional resources on the Addgene blog Subscribe to the Addgene blog Find lab tips for your research...

33. Oh, The Places You Can Go: Careers in Science Communication - Product Development

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    Blog Post

    ...a science education company. And make sure to subscribe to the Career Channel of the blog so you don’t...

34. Oh, The Places You Can Go: Careers in Science Communication - Science Writing

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    Blog Post

    ...in future career blog posts. And make sure to subscribe to the Career Channel of the blog so you don’t...

35. FastCloning: A Newer, Simpler Cloning Technique

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    Blog Post

    ... the correct size (vector + insert). You should screen 3-4 colonies to ensure that you find the correct...

36. Sleeping Beauty Awakens for Genome Engineering

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    Blog Post

    ...primary cells. In 2009, Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity ...

37. Management for Scientists: Giving Feedback

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    Blog Post

    ...a 5 part series on Management for Scientists. Subscribe to the Addgene Blog to follow the entire series...

38. Plasmids 101: Antibiotic Resistance Genes

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    Blog Post

    ...Plasmids 101 Posts Learn How to Use Blue White Screening to Simplify Your Cloning Experiments See Where...

39. RUBY-Red Siliques

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    Blog Post

    ...to select against CRISPR positive plants, but screening these can be challenging and requires specialized...

40. Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource

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    Blog Post

    ... advances in gene synthesis, affinity reagent screening, and next-generation sequencing of hybridomas ...

41. Plasmids 101: Origin of Replication

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    Blog Post

    ...p15A B Relaxed pSC101 ~5 pSC101 C Stringent pBluescript ~300-500 ColE1 (derivative) and F1** A Relaxed...

42. Meet the New Addgene Board Members!

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    Blog Post

    .... “They took a dividing yeast and did a genetic screen to identify the factors that regulate cell division...

43. New Viral Vectors - Winter 2025

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    Blog Post

    ...delightful. Grab a hot beverage and a blanket, then scroll on for some wintery delights!    Table 1: New...

44. Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC

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    Blog Post

    ...immunocytochemistry (ICC). Not all of the solutions described here may be as effective for other techniques,...

45. Multifunctional Neural Fibers: Modulation from Gut to Brain

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    Blog Post

    ...using NeuroStack and the fibers as previously described, then measured electrophysiological potential ...

46. What the HEK?

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    Blog Post

    ...with kidney cells (Shaw et al.). Since then, transcriptome studies of the cell line have determined it ...

47. Identifying Sequence Elements with SnapGene's Feature Database

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    Blog Post

    ... for control elements such as promoters and transcription terminators, the boundaries are less obvious...

48. What Do I Do Now? Academic v. Non-Academic Career Decisions

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    Blog Post

    ...frustrating for solo flyers Other people will routinely scrutinize your data—science can be surprisingly more rigorous...

49. Using Addgene's New Viral Service for Your Research

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    Blog Post

    ...modulate its expression, or perform a genome-wide screen, we’ve got viruses to help you out. Of course, ...

50. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

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    Blog Post

    ...rRNA in bacterial RNA-Seq. This enriches for transcripts of interest without an increase in the number...