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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 551 results for: ADA

Showing: 181 - 200 of 551 results

  1. Hot Plasmids: Fall 2024

    Type
    Blog Post
    ...ubiquitination substrate Ikaros (IKZF1ZF2) (B). Image adapted from Kroupova et al., 2024 under CC BY license....design and studies of protein ubiquitination and degradation.   Find the CRBNmidi plasmid here!   Kroupova...

  4. To Codon Optimize or Not: That is the Question

    Type
    Blog Post
    ...the late 1980s, scientists even created a codon adaptation index (CAI) that is based on the codon usage ...10.1038/nrg3051 Sharp PM, Li W-H (1987) The codon adaptation index-a measure of directional synonymous codon...

  6. Bioinformatics at Addgene

    Type
    Blog Post
    ...have been developed for a singular purpose (ex. adapter trimming, quality score analysis, k-mer counting...Java, R, etc.) in our pipeline. Crucially, this adaptable property gave us the freedom to use multiple open-source...

  7. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...your problem. In a study outlined in Originals by Adam Grant, a creativity expert at Stanford conducted... with the group (because they will fight more adamantly for their solution), selecting a devil’s advocate...

  8. Hot Plasmids - November 2023

    Type
    Blog Post
    ...trafficked from the “source” to “destination”. Images adapted from Lee and Cheah et al. 2023 bioRxiv (A) and ...:GFP-iPAK4 at 3 or 6 h. Scale bar 5 µm. Images adapted from Lin et al., 2023 bioRxiv, under CC-BY-NC 4.0...

  11. New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor

    Type
    Blog Post
    ...SF-Venus-iGluSnFR.A184V, iGluSnFR3 v82, and iGluSnFR3 v857. Image adapted from Aggarwal et al., Figure 1   Increased...curves for the different iGluSnFR variants. Image adapted from Aggarwal et al…, Supplementary Figure 3. .../F0 for the different iGluSnFR variants. Image adapted from Aggarwal et al…, Supplementary Figure 2. ... PDGFR, GPI, and SGZ display constructs. Image adapted from Aggarwal et al… Figure 4. PDGFR is a C-terminal...

  12. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

    Type
    Blog Post
    ...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...Chatters, S. J., de Ridder, D., Gilmour, K. C., Adams, S., Thornhill, S. I., Parsley, K. L., Staal, F....

  13. Hot Plasmids: Summer 2024

    Type
    Blog Post
    ...structure of the H1.8-GFP-bound nucleosome. Images adapted from Arimura et al. 2024 under CC-BY-NC-ND 4.0 ...3D map with 3.1 Å global resolution (E). Figure adapted from Abe & Lim, 2024 under CC-BY-NC-ND 4.0 license...

  14. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    ...twist since it relies on the sequential nicking, degradation, and PCR-generation of a mutant version of the...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138...

  15. Design Tips for Prime Editing

    Type
    Blog Post
    ... motifs at their 3′ end to protect them from degradation. When designing epegRNAs with large structured...different strategies for protecting pegRNAs from degradation. Created with BioRender.com. That’s all ...A., Sahin, M., Osborn, M. J., Weissman, J. S., Adamson, B., & Liu, D. R. (2021). Enhanced prime editing...., Bauer, D. E., Marson, A., Parsons, L. R., & Adamson, B. (2024). Improving prime editing with an endogenous...

  16. Open Resources and Plasmid Tools For Studying C. elegans

    Type
    Blog Post
    ...for the Auxin Inducible Degradation System (AID) The Dernberg lab in 2015 adapted the AID system discovered...and new tools for conditional expression and degradation. These workshops not only highlighted new tools...  Zhang, Liangyu, et al. "The auxin-inducible degradation (AID) system enables versatile conditional protein...

  17. Hot Plasmids: Winter 2025

    Type
    Blog Post
    ...weeks after herbicide was applied to both plants. Adapted from Vu et al., 2024 bioRxiv, under CC-BY-NC-ND... B) Protein expression and labeling procedure. Adapted from Smith et al. 2024 under a CC-BY 4.0 license...(C) is indicated; red line is exponential fit. Adapted with permission from Hao and Lee et al., 2024. ...non-neurodegenerative diseases (Diabetes & cancer). Image adapted from Wu and Wang 2024 under a CC-BY 4.0 license...

  18. Isolating Genomic Regions of Interest with the CRISPR System

    Type
    Blog Post
    ...purification of a specific genomic region, numerous adaptations of this system have been made in different fields...specific genomic region has also recently been adapted to isolate genomic loci in bacteria (Fujita et ...also has great potential for improvements and adaptations that can provide an increased understanding of...programmable dual-RNA–guided DNA endonuclease in adaptive bacterial immunity." Science337.6096 (2012): 816...

  19. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...endocytosed material or molecules targeted for degradation. These two pathways also use different cytoskeletal...several parallel mechanisms in place (for review see Maday et al., 2014). It is currently unclear which proteins...Common AAV Serotypes and their target tissues (adapted from Addgene's AAV guide)  Commonly used serotypes...PMID: 22196330. PubMed Central PMCID: PMC3275419. Maday, S., Twelvetrees, A.E., Moughamian, A.J., and Holzbaur...PubMed Central PMCID: PMC4269290. Masamizu, Y., Okada, T., Kawasaki, K., Ishibashi, H., Yuasa, S., Takeda...

  20. Hot Plasmids: Spring 2025

    Type
    Blog Post
    ...Rising τ, and Decay τ of the three sensors. Image adapted from Aggarwal et al. 2025 under a CC-BY-NC-ND 4.0...displaying characteristic PV basket cell morphology. Adapted from Furlanis, Dai, Leyva Garcia et al. 2024 bioRxiv...
Showing: 181 - 200 of 551 results