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  1. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...

  3. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...

  4. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...

  7. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ...

  10. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...

  12. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...

  14. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived...

  15. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...

  16. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type
    Blog Post
    ...human disease. However, the rate of transfer was variable. They measured transfer rates by comparing the... of a constitutely expressed RFP reporter, was variable across bacterial strains, ranging from an ~8 to...

  19. Transferable Skills: Negotiation

    Type
    Blog Post
    ...covered separately in a future post.   Resources Maria recommends: Getting to Yes: Negotiating Agreement...  Maria Genco...

  20. Pooled Library Amplifications

    Type
    Blog Post
    ...unique plasmids does your library contain?), the variance of size of plasmids in your library (Are the inserts... Library Type Name Number of Plasmids Variable Region Size DNA per Amplification Amplification...
Showing: 181 - 200 of 382 results