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Showing: 181 - 200 of 391 results
  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    ..., large collections of biological independent variables, are becoming increasingly popular tools with ...your library cargo to isolate the effects of each variable being manipulated. Thus, the adjustable nature...
  2. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...Japanese firefly (Luciola cruciata), and more. These variant proteins offer different kinetics, substrate requirements...measurements of luminescence from live cells. Luciferase variants with incompatible substrates can also allow multiplexing...
  3. RNA Extraction Without A Kit

    Type
    Blog Post
    ...purification. There is one simple method that works, and variations to that method. A major hurdle to developing ...isolate RNA from 20+ hours to around 4 hours, and variations on this no-kit method are still widely used today...
  4. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    ... application. Consider also that there may be variations within an application. Take immunohistochemistry...might find that while the website highlights a variation of your application, a publication successfully...
  5. Antibodies 101: Polyclonal Antibodies

    Type
    Blog Post
    ...polyclonal antibodies is their batch to batch variability. Once an animal has undergone a terminal bleed...antibody instead. Otherwise, you might experience variability in the strength of staining and background noise...
  6. Plasmids 101: Cre-lox

    Type
    Blog Post
    ...lox2272 (GgATACtT), and lox511 (GtATACAT). These variant lox sites undergo recombination with other sites...not cross-compatible. Using different lox site variants allows Cre to catalyze more than one specific ...
  7. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    ...cross-reactivity (binds to non-target proteins), variability (separate batches can perform differently – happens...This homogeneity circumvents the batch-to-batch variability found in polyclonal antibodies, creating a potentially...
  8. Viral Vectors 101: Calcium Sensors

    Type
    Blog Post
    ...between experiments can be more difficult due to variance in sensor expression if you aren’t working with...through FRET sensors, can reduce these unwanted variables. Twitch is a FRET-based calcium reporter derived...
  9. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ... homozygous at nearly all loci. This reduces variability in genotype and phenotype and helps improve the...the cells in which Cre is expressed.  Several variations of this technology can be used to customize the...
  10. The Materials Science of Optogenetics Experiments

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    Blog Post
    ...materials and methods of a paper that uses the variant you wish to use to determine the ideal excitation... be controlled. As discussed above, each opsin variant has slightly different activation/inactivation ...
Showing: 181 - 200 of 391 results