...2013 (7, 8). Nine months later, more than 1500 publications describe work to improve the tool’s specificity... providing tips from experts in the field, and enabling access to multiple plasmids used for various platform...

... in synthetic biology and DNA synthesis. They published their results in a paper in Nature Communications... Nielsen & Christopher A. Voigt also recently published a tool (trained on Addgene plasmid datasets) to...

...Interviewee: “I did the most work for a paper we were publishing, but when it came time to add author details ...which had affected his judgement. Eventually, we published the paper in a high-impact journal and it worked...

...how the antibodies work in various applications, enabling you to better plan your experiments. To promote...Bandrowski et al., 2016). It’s also been well established that recombinant antibodies are excellent for...

...video of her singing about the paper recently published by her lab and Mustafa Khammash's lab at ETH Zürich...UriAlonWeizmann, here my song about our paper recently published in @nchembio Title: Bye bye L-arabinose drive....

...Originally published Feb 6, 2014 and last updated Nov 10, 2020. Now that we know all about antibiotic...ori is the place where DNA replication begins, enabling a plasmid to reproduce itself as it must to survive...

...at Addgene have gone virtual!   2. Practice public speaking For almost any science career, there will... will be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving...

...several years ago we partnered with Seqwell to establish a next-generation sequencing (NGS) QC platform...serotype of our AAV preparations. We recently published a description of the VGS process and use cases...

...these tools, which aren’t always easy to find in publications, so this is a much-needed community resource...which is equally important to report. We recently published a paper in Frontiers in Neuroanatomy and summarized...

...promise to be host-independent, beyond the oft published metabolic engineering realm of S. cerevisiae and...library of characterized aptamers and supporting publication: http://www.freebase.com/base/aptamer Cruz-...

...sensitivities can now be applied to dozens of other published FRET-based sensors. For example, his team is now... improve upon an indicator they developed and published in 2010 for visualizing the transcription factor...

...than NHEJ. ID Plasmid Gene/Insert Promoter PI Publication Browse CRISPR Plasmids By Function Genome Editing...opposite strand) mutations. ID Plasmid Promoter PI Publication Nick CRISPR/Cas nickase mutants introduce gRNA-targeted...repair (HDR). ID Plasmid Gene/Insert Promoter PI Publication Prime Edit Cas9 H840A nickase fused to a reverse...template. ID Plasmid Gene/Insert Promoter PI Publication Activate Catalytically dead dCas9 fused to a ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication Interfere Catalytically dead dCas9, or dCas9 ...specific locus. ID Plasmid Gene/Insert Promoter PI Publication RNA Targeting Type VI CRISPR systems, including...the entire population. ID Plasmid Promoter PI Publication Purify A catalytically inactive Cas9 (dCas9) ...

...identified MerMAIDS from their analysis of different publicly available metagenomic data including the Tara ...demonstrates the power of combining the analysis of publicly available metagenomic datasets with biophysical...

...researchers are open to sharing knowledge and establishing collaborations, so it doesn’t hurt to reach ...expect to generate data that will contribute to a publication? A grant? A patent? It is never too early to ...

...this screen successfully differentiated cells resembling all three germ layers (ectoderm, endoderm, and...amplification, refer to the Zhang lab’s previously published protocol and its relevant modifications on the...

...PMCID: PMC6070313. Find the plasmids from this publication at Addgene! Additional Resources on the Addgene...pages Find plasmids from the BioBits BrightTM publication here ...

...Do-it-Yourself (DIY) biology laboratories working towards enabling everyone to participate in the biotech revolution...detect compounds in the environment, and openly publish protocols so that anyone can engineer yeast to ...

...vectors VSV-G and psPAX2, which were used in a publication by Xiuyuan Ou, et al. that characterized the ... requested these plasmids from Addgene. This publication then resulted in the development of a new plasmid...

...do just this.  In under a month’s time, we’ve published an article on BioRxiv describing a comprehensive...coronavirus, one FedEx package at a time Since the publication of our paper, our group has been inundated with...

...libraries are popular tools for genetic screens, enabling genome-wide interrogation of the genes required...are refractory to lentiviral transduction, now enabling forward genetic screens in cells with few genetic...