We narrowed to 214 results for: DAP

1. Plasmids 101: Codon usage bias

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   Blog Post

   ...structures in 10 closely related yeast strains. Adaptation to changing conditions - Organisms often need...

2. Five Popular Model Organisms, Part 2

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   ...plant species and metabolic properties affecting adaptations.  Arabidopsis thaliana flowers. Image from...

3. Adeno-associated Viruses (AAVs) for Genome Editing

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   ...and pAAV-TK-Acceptor) so that they can be easily adapted to edit your gene of choice. These vectors are ...

4. Multicolor Animals: Using Fluorescent Proteins to Understand Single Cell Behavior

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   ... visualization of two colors. Similarly, Flybow adapts Brainbow 2.0 for use in Drosophila melanogaster...

5. Plasmids 101: Biotinylation

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   .... The biotin-avidin interaction has also been adapted for use in immunohistochemistry and antibody labelling...

6. Deep Mutational Scanning with One Pot Saturation Mutagenesis

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   ...Natarajan, Chandrasekhar, et al. "Epistasis among adaptive mutations in deer mouse hemoglobin." Science 340.6138...

7. Viral Vectors 101: Integrase-Deficient Lentiviral Vectors

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   ...non-integrating transgenes. Created with BioRender.com; adapted from Banasik and McCray, 2010. These episomes...

8. Antibodies 101: Normalization and Loading Controls for Western Blots

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   ...the gel (n=3). Data is presented as mean ± SEM. Adapted from Analytical Biochemistry, vol 440, Gilda, J...

9. Plasmids 101: Gibson Assembly and Other Long-Homology Based Cloning Methods

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   ...parts. Gibson assembly meets CRISPR Gibson can be adapted to more complicated cloning schemes, such as those...

10. Treating Muscular Dystrophy with CRISPR Gene Editing

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    ...low-frequency indels will not cause frameshifts. Figure adapted from Osterout et al. (2015b) under a CC-BY 4.0 ...

11. Technologies Enabled by NanoLuc® Luciferase

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    ...accuators. The researchers took a FRET reporter and adapted it into a BRET reporter by using NanoLuc® Luciferase...

12. Antibodies 101: Multiplex Immunofluorescence

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    ...C3 show magnified detail of the box in C. Image adapted from Andrews et al., 2019 under CC-BY 4.0 license...

13. Technique: Probe Phage Genomes for Host Binding Proteins

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    ...Mohammadali Khan Mirzaei, and Anders S. Nilsson. "Adapting drug approval pathways for bacteriophage-based...

14. Building Global Connections with the International Mentorship Program USA-EUROPE

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    ...mindedness, and the ability to accept feedback and adapt their mentoring styles to the mentee’s personality...

15. An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency

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    ... editing methods, other Cas proteins have been adapted for use in C. elegans. In 2017, the Korswagen lab...

16. Mycoplasma Contamination: Where Does It Come From and How to Prevent It

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    ...staining alternative. This method uses DNA stains like DAPI and Hoechst 33258 to stain all DNA. Some amount ...

17. Important Considerations When Using AAVs

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    ...Skeletal Muscle AAV1, AAV6, AAV7, AAV8, AAV9 Adapted from https://www.addgene.org/viral-vectors/aav/...

18. Plasmids 101: Using Transposons in the Lab

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    ...closer look at three different transposon systems adapted for use as research tools: Sleeping beauty, PiggyBac...

19. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

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    ...ICC) as a percentage of total cells in the field (DAPI). Using the ICC assay as a direct readout of tag...

20. Antibodies 101: Designing Your First Flow Panel

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    ...fluorophores in relation to the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-...