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... what we are accomplishing and what more we might do. I am grateful every day for their dedication, flexibility...worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year Boston...working here and I hear from the Addgenies that they do too. Ever watchful, but I’m so proud of our culture..., we also don’t have to worry about being able to do good in the world. We are making an impact.  {% video_player...

...legal status of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits...setup, there are no explicit instructions on what to do when something fails. While we’re sure our techs ...have to individually keep track of any failures and do troubleshooting on their own. For a few samples this...everyone up at some point, decide early on what to do in a namespace collision. It may even be worth it...

...stability Figure 1: So many choices! What to do? The main factors that can affect DNA stability...stability of your DNA, it will limit what you can do with these samples. This is because divalent cations...than water in these conditions.  The next time you do a plasmid prep, plan in detail before you drop that...

...researchers are communicating across disciplines or doing different tasks. But these challenges can be exaggerated... with each other in person. One possibility is to do a lab exchange, where researchers spent time in each...cohort, neither of which I would have been able to do from my home lab. Scientific conferences and workshops...

...continue reading for more details. How much time do you spend reading scientific literature? Very often...’t save you the hard work of reading papers and doing experiments but we can definitely give you credit...envisions a world where the impact of teaching and doing science is rapid, concrete and open to innovation...

...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of...you’re seeking. Fortunately, there are many ways to do this involving positive selection, negative selection...cells that contain a specific gene survive. The rest do not grow. While there are many ways to design your...

...determines that our current queue is too high and we do not expect to meet the general turnaround time, Addgene...

...compelling, and I encourage you to hold one. Why do we need physical models of protein structure? Structural... you can only wonder what I am talking about. How do researchers use physical models of protein structure...and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We...human body?” … and probably most importantly… "how do we know this?” Over the past fifteen years, the CBM...

...Entrepreneur, Motivational Speaker Do you have a difficult supervisor, or do you have to work with difficult...problem and leave your emotions out of the discussion Do not assume that your supervisor is familiar with ...ideas, but he or she probably has reasons for it. Do not take criticism personally or get defensive. Look...

...’t truly address the question of what I wanted to do when I grew up until I was all but finished my undergraduate...curiosity did not ultimately translate to a desire to do the research itself. Several years of exclusively...audience and how to utilize a wide array of media in doing so—can prove invaluable in the pursuit of public...

...transition state or specific configuration. So how do you ensure that you have the right antibody for the...configuration in your assay. Did you have a denaturing step? Do you want to capture the protein bound to anything...elucidate information about what a given protein is doing or changing in different conditions. Just remember...

...primary antibody controls in your experiments. If you do find high levels of background staining by the secondary...binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your secondary antibody bind endogenous...common solutions to avoid background staining when doing species on species IHC. (A) One solution is to use...

...2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions The answer is, yes, it does...Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound to an...structure and isotype determination, remember that it can do so much more than that!    References and resources...

...Enhancer traps do not need to be integrated within an intron or exon of a gene, and thus they do not disrupt...they are directly integrated into it. Enhancer traps do not need to be inside of a gene’s open reading frame...

...alone; a researcher doing some experiments in Brazil can have the same issues as one doing experiments in ...sometimes wish they could live differently than we do day to day. We discovered that it can be good for...

...“Synthetic biology, in particular, the ability to do DNA synthesis, is evolving so fast that it is a really...technological change happens really fast,” Thompson says. To do this, Thompson reached out to MIT labs in the Synthetic... engineered? Where did it come from? What does it do? Those are the sets of questions one would want to...

...with a PhD in biology. Depending on what you want to do, that’s not necessarily bad news, but it is an argument... science with the community. Some of them you can do today! 10 ways to share your science 1)   If you ...talk – really talk – about the research you are doing in the lab with your mother or father or your best...

...support your career development as your mentor. How do you make the most of this new relationship? Consider...skills in which you have expertise or knowledge. Where do these intersect? Work with resources: Choose books...take larger goals (e.g. Figure out what I want to do after I get my PhD) and break them down into smaller...

...questions! How much virus should I inject? How long do I need to wait? What serotype and promoter should...the answers, but we’re betting that your colleagues do. That’s why we’re launching the AAV Data Hub. The...

...the population that have an edit. I didn’t do this, but to do it you would need a pretty good gel image...Cas9-expression cell line. Especially if you’re doing a screen. I made a few monoclonal lines (Figure ...expansions (monoclonal, 1-5). Wild-type A549 cells do not express CAs9 and are included as a control (WT...couldn’t get a good image. You could probably also do Western blotting against GFP expression, but I didn...designing primers flanking my edits in genomic DNA and doing PCR on genomic DNA. The surveyor assay itself was...