...in orange. Created with BioRender.com.    How do secondary antibodies work?  Secondary antibodies ... such as the rabbit IgG class of antibodies. They do so by targeting the Fc region of an antibody, the...But in reality, multiple primary antibodies can and do attach to a single target protein, so this assumption...exception of flow cytometry, most immunoimaging assays do not allow for absolute quantitative analysis of a...

... if you have your first cold of the season like I do, sniffle) ourselves back into our regular post-holiday...other people. (We don't need evidence for that one, do we?) OK, so, now that we’ve convinced you that laughter...

...fill this gap by both highlighting that researchers do learn MANY transferable skills while working in the...teams in house to solve every problem (although we do that too), we will often look for partnerships where...the established partnership. If you’re willing to do similar things for your team or lab, you’re likely... forget about collaborations and projects you can do with others after work. Get sponsors for a training...job someday. Universities themselves can help you do this through practical tools like alumni networks...

...with fluorophores whose excitation/emission spectra do not overlap. Multiplexed IF can also require some...the performance of other antibodies. But, once you do find the right protocol, multiplex IF can give you...use and the ultimate outcome of your assay. So how do you choose good ones? Choosing a secondary is often...available imaging instruments and the spectral properties do not overlap with any other fluorescent molecules ...antibody will likely perform as expected in both.  How do you find validated antibodies? Many companies that...

...this heyday of molecular biology, many scientists do a lot of DNA work but never get to actually manipulate... - birds eat a caterpillar once, vomit, and never do it again. Cichlids (Scott Juntti) Cichlids are 2,...open so you can see them and even feed them RNA to do silencing in the wild. They also return to their ...they are trying to replace embryo injection (hard to do, expensive, not every lab has the capability) with...developed and shared for many different organisms. Do you have a favorite organism you study in the lab...

...ll be able to do it when needed. How many people from your lab would use the ELN i.e. do you need a system... that we don’t forget to write things down when doing scientific research? Electronic lab notebooks and...different lab members to avoid any issues later on. Do you need external teams or partners to access your...specific permissions set for every member of your team. Do you need to make sure your research can be patented...

...Graduate Student Career Outcomes University websites do not currently publish career outcomes for PhDs or...those required at the lab bench. At Addgene we try to do our part in boosting awareness of various career ...Write Often Whether you take on opportunities to do some blogging, write reviews, or write for a university...certainly true that not all PIs can take the time to do this, you should always reach out to your peers and...questions that needs to be asked going forward is: How do we measure inclusion and equity? This is a difficult...

... at Experiment Designs Consulting, Inc. “How much do you need [for your lab start up]?” A hiring chair...tools Outline the knowledge and skill sets needed to do your science. This will help you identify the talent...if you’re going to need a tech because you’ll be doing mouse experiments, start defining keywords you’ll... and resources that you’ve thoroughly identified. Do online searches, and seek advice from your current...resources. For example, if a dean/chair asks “how much do you need to start your lab”. You can respond by saying...

... luciferase in mammalian cells Wong Return to Top Do you have suggestions for other plasmids that should...

... what we are accomplishing and what more we might do. I am grateful every day for their dedication, flexibility...worry a very little bit about hiring when we need to do it quickly. Hiring is hard but as a 3 year Boston...working here and I hear from the Addgenies that they do too. Ever watchful, but I’m so proud of our culture..., we also don’t have to worry about being able to do good in the world. We are making an impact.  {% video_player...

...legal status of thousands of plasmids a day, like we do at Addgene, pen and paper will fail you. The benefits...setup, there are no explicit instructions on what to do when something fails. While we’re sure our techs ...have to individually keep track of any failures and do troubleshooting on their own. For a few samples this...everyone up at some point, decide early on what to do in a namespace collision. It may even be worth it...

...continue reading for more details. How much time do you spend reading scientific literature? Very often...’t save you the hard work of reading papers and doing experiments but we can definitely give you credit...envisions a world where the impact of teaching and doing science is rapid, concrete and open to innovation...

...stability Figure 1: So many choices! What to do? The main factors that can affect DNA stability...stability of your DNA, it will limit what you can do with these samples. This is because divalent cations...than water in these conditions.  The next time you do a plasmid prep, plan in detail before you drop that...

...researchers are communicating across disciplines or doing different tasks. But these challenges can be exaggerated... with each other in person. One possibility is to do a lab exchange, where researchers spent time in each...cohort, neither of which I would have been able to do from my home lab. Scientific conferences and workshops...

...successfully takes up plasmid DNA. Among those that do, some now have plasmids that contain your gene of...you’re seeking. Fortunately, there are many ways to do this involving positive selection, negative selection...cells that contain a specific gene survive. The rest do not grow. While there are many ways to design your...

...compelling, and I encourage you to hold one. Why do we need physical models of protein structure? Structural... you can only wonder what I am talking about. How do researchers use physical models of protein structure...and dangerous. Subscribe to CRISPR Blog Posts How do educators and students use molecular models?   We...human body?” … and probably most importantly… "how do we know this?” Over the past fifteen years, the CBM...

...Entrepreneur, Motivational Speaker Do you have a difficult supervisor, or do you have to work with difficult...problem and leave your emotions out of the discussion Do not assume that your supervisor is familiar with ...ideas, but he or she probably has reasons for it. Do not take criticism personally or get defensive. Look...

...mouse strains). AAV-PHP.eC, AAV9-X1.1 and AAV1-X1 do not display strain-specific tropism and can be used...choose the best capsid for your cell type. First, do you want to target CNS or PNS? Then consider...

...primary antibody controls in your experiments. If you do find high levels of background staining by the secondary...binding Direct immunohistochemistry (Figure 2A)  How do you avoid having your secondary antibody bind endogenous...common solutions to avoid background staining when doing species on species IHC. (A) One solution is to use...

...2016; Torres & Casadevall, 2008) but…does it really do anything? Fc functions The answer is, yes, it does...Fc effector functions and all that antibodies can do! For instance, some Fc receptors, once bound to an...structure and isotype determination, remember that it can do so much more than that!    References and resources...