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We narrowed to 824 results for: EED

Showing: 181 - 200 of 824 results

  1. Phage Directory: From Phage Therapy to a Repository of Phage Information

    Type
    Blog Post
    ... whose infection has failed all #antibiotics. We need lytic non-lysogenic phage URGENTLY to find suitable...help clinicians and scientists quickly find much-needed phages for patients.   A phage directory is crucial...chance that an early request for phage may not be needed if other antibiotics work. “We don’t want to cry...phage community until we have someone that really needs it, but then it might to be too late,” Sacher says...treatment occurs. In addition to providing much needed information and potential for successful phage ...phage therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires...

  2. A Better Way to Get Customer Support: The Help Center

    Type
    Blog Post
    ...waiting multiple days for a reply to an email. You need answers: the right ones, quickly, and this is why... to make our support team available whenever you need them, but sometimes that’s not enough. With your...and make a phone call or write out an email. You need help right at the moment you’re looking for it, ...Center to make sure that, whatever your support needs are, we’ve got you covered! In addition to the Help...making it easier than ever to get the support you need. If for some reason you can’t find the answer that... the support we provide based on your valuable feedback. This is Addgene after all, and making your research...

  3. A Control for All Seasons

    Type
    Blog Post
    ...: Validation post. The primary antibody control needs only to be run once for each primary antibody that...that will be used (Burry 2011). It does not need to be repeated for each experimental run as long as the...addition to the above-mentioned safeguards, you’ll need to test the secondary antibody in the absence of...detected, the level of noise will determine whether it needs to be addressed. In order to correctly interpret...interpret your results, the signal from your target needs to be significantly higher than the background noise...antibodies in the stain. In situations like this, you may need to find alternative primary or secondary antibodies...samples in different lanes in a western blot, you’ll need to include a loading control. A loading control ...

  4. The Strength of Story Telling

    Type
    Blog Post
    ...them You don’t need to explain as much of your science in a weekly lab meeting as you’ll need to for an outsider...telling it. End at the end A story needs a good start point and it needs a good end point. One thematically... a Good Story Start at the start Any good story needs a solid point to kick off at. Whatever you’re working... outsider. And you won’t need to dive as deep into your research at an outreach event as you might in ...

  5. Summer SciComm: Science Storytelling

    Type
    Blog Post
    ...audience can relate to. It doesn’t need to be anything fancy; it just needs to capture your audience’s attention...competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent tension...(nonfiction) story. For there to be a story, you need to have conflict. There are a lot of ways to introduce...the Conflict  Once you have your conflict, you’ll need two other things to complete your story: an introduction...listen or watch) further.  Second, the introduction needs to provide enough information so that the audience...

  6. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ...101: Control Plasmids Need help thinking about the types of controls you’ll need for your next experiment...posts aim to give researchers the background they need to get started using plasmids and the revolutionary...Scientists: Why and Where Do I Find One? If you need help making a transition into a new job or maybe... CRISPR/Cas9 to make a change to the genome, you need to check whether you’ve made the right one. In this...us through some common controls and why you might need them. Happy Reading!   Resources on the Addgene...

  7. From Lab Bench to Leadership: Transitioning into Management as a PhD

    Type
    Blog Post
    ...quickly learned that my approach needed to adapt based on individual needs. For instance, I had one colleague...shift and navigate the pitfalls? Here's what you need to know.   Figure 1: Management can seem ...steps to a lab assistant. However, I recognized the need to shift my mindset to empower my team and allow...highly self-motivated and excelled when given the freedom to explore projects autonomously. Failing to recognize...managers fail to set expectations or provide timely feedback, leading to unproductive dynamics. I experienced...

  8. Quick Guide to Working with Drosophila Part 3: Genome Engineering in Flies

    Type
    Blog Post
    ...system, but the available reagents do not match your needs. If YFG is thrillingly new, you might have to make... germline. To begin this process, you will first need to do some molecular cloning. Depending on whether...trying to overexpress or knockdown a gene, you might need different plasmids. Harvard’s DRSC/TRiP Functional...different fly lines. Also, if there is a chance you need to combine multiple transgenes in one fly, having...express multiple transgenes, remember that you’ll need to integrate them all at different loci. Fortunately...for use? Random insertions take longer because you need to determine in which chromosome your transgene ...

  9. Sweating the Small Stuff: Details in the Lab

    Type
    Blog Post
    ...example, I used to constantly underestimate time needed to prep for an experiment (labeling tubes never...after running a protocol a few times, you no longer need the full checklist. Great! But don’t just stop using...into one. Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   ...they use them.    Prevent Interruptions If you need to focus, a large set of headphones can act as a...wall of pipette tip boxes when she particularly needed to focus, which was extremely effective. Others...at the bench. Don't be afraid to ask for what you need to be successful! Slowing Down At the suggestion...

  10. Will You Be My Mentor? Finding and Asking for Mentoring Support

    Type
    Blog Post
    ...means the mentee needs to be open to change and constructive suggestions and feedback. The best thing ... able to: Help you identify specific skills you need for your current position Understand your interpersonal...of the people you work with Give more informed feedback (for example, they may be able to hear you present...elsewhere is able to: Help identify the skills you will need to make a change Provide perspective on core values...at another company/department Give more candid feedback and be able to discuss more confidential topics...

  11. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ... have its limitations or that other technologies need to be discarded but clearly we are in an era of ...focuses primarily on behavior, you don’t necessarily need to use electrophysiology to prove that your optogenetic...required. In any given optogenetics experiment you may need to inject virus into 20 or more mice and the injections... die injection prior to using the virus. You may need to adjust coordinates based on your own method of... limited by the mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive...terminal also affects the length of time you may need to allow the virus to express. For example, if you...surgeries and viral expression, you are ready to proceed to the material science part of optogenetics. In...

  12. Antibodies 101: Fab Fragments

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    Blog Post
    ...Fab fragment, it either needs to be directly conjugated to a reporter or you need a light-chain specific...antibody. Fab fragments are useful when you just need an antibody’s antigen binding function but not the...antibodies, you can make your own antibody fragments as needed.  In contrast, anti-IgG antibodies, commonly used...fragments make good secondaries for cases where you need to pre-form primary-secondary antibody complexes...about what pieces of the antibody you do and don’t need, and how the protein will interact with other components...

  13. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

    Type
    Blog Post
    ...stabilized promoters incorporate feedback loops such as the incoherent feedforward loop (iFFL). In general, iFFLs...to interpret or can interfere with the precision needed in metabolic engineering or other synthetic biology...deoxychromoviridans requires three genes whose proteins are needed sequentially. When Segall-Shapiro et al. placed... In cases where a specific ratio of products is needed, such as within a multi-protein complex, stabilized...between the different components. When your pathway needs precise fine-tuning of gene expression levels, give...

  14. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    ...primers, or mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection...of your lab can access and use the materials they need, and avoid duplicating your effort. In addition,... it will be easy to find all of the information needed for the process. Always sequence verify Once the...sometimes more depending on the number of features we need to verify. Determining which features to verify ...Remember, when designing a sequencing primer, you need some space between your primer binding site and ...high-throughput plasmid sequencing If you’re like us, you need a strategy for processing several sequencing reactions...

  15. How to Deposit Your Plasmids with Addgene

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    Blog Post
    ...deposit process. To deposit plasmids online, you will need to create an account with Addgene before starting...depositing 10 or more plasmids. (If you believe you need to deposit a collection that is greater than 75 ...contains columns that correspond to the plasmid data we need; you can copy and paste your information directly...antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify any genomic alterations...modify the gene/insert and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to ...entry fields to describe the next insert. Repeat as needed for more inserts. When you are finished entering...addgene.org. Include your deposit number and which data needs to be updated. When your plasmids arrive at Addgene...

  16. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    ...many cases where the specific viral particles you need are not available, or the cost of custom viruses...too high for your budget. In those cases, you’ll need to produce your viruses in-house.   Introducing...trying to make. For example, bacteriophages will need to be produced in bacteria cells. However, some ... producing a stock P1 virus, so that if you ever need to remake the same virus, you can skip the first... just two days, but add additional costs and may need to be optimized for your purpose. Making your decision... Baculovirus; can produce AAV and lentivirus if needed # of plasmids Three or four Two Packaging limit...

  17. Sharing Your Lab Protocols: Using Apps to Save Time & Track Your Experiments

    Type
    Blog Post
    ...start-ups are turning to software apps to solve our need for quicker, more effective updating and dissemination...protocols when they begin their own experiments. Need to submit an updated protocol? Just email us at ...departments, industry, and more to fit every lab's needs. Plus there are the apps - many to help you access...solution to your lab's organization and management needs. With Quartzy you can share protcols with your labmates...your thoughts on how the app works for your lab’s needs. At Addgene, we’ll continue to create new molecular...and wikis - by Rory Macneil on Bitesize Bio Data Freedom: The Expansion of Data Sharing in Research Publications...

  18. Twenty Years of Sharing: Expanding Accessibility

    Type
    Blog Post
    ...customs processes, and the distances the packages needed to travel meant the accessibility varied country...showing exactly how to execute a step can provide needed clarity (Table 1). Our most popular video, however...measure of our educational resources’ value is feedback from the researchers who use them. The best part...out to ask if they can help us create or update a needed resource, and when they tell us how they use our...to enable virtual learning during the pandemic. Feedback like that lets us know that Addgene’s materials...internationally, after all, that we could identify the need for distributors in some countries. ‌We’ve developed...

  19. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    ...own expression, so high levels of DnaA act as a feedback mechanism to reduce further transcription. Replication.... Fun fact! As far as we can tell, DnaA doesn’t need to hydrolyze ATP to do its job — although ATP does...for a given plasmid has been reached, negative feedback circuits reduce replication of the plasmid to ...plasmid ori, after which replication of the plasmid proceeds (and terminates) similarly to the bacterial chromosome...controls the copy number of pSC101 through a negative feedback cycle. As with DnaA, high concentrations of RepA...given plasmid influences how high the copy number needs to get before handcuffing occurs. These mechanisms...

  20. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    ...this problem in C. roseus by working with young seedlings and optimizing several other aspects of the transient...D'Aoust et al., 2009): Usually a syringe without a needle is used to inject an Agrobacterium culture through... a great help in screening candidate genes and speeding up research. Certainly, there is still more to...Ching-yeh, et al. "Intrinsic GUS-like activities in seed plants." Plant cell reports 9.1 (1990): 1-5. PubMed...method for transient gene expression analysis in seedlings of Arabidopsis and other plant species." Plant...analyzing gene function in Catharanthus roseus seedlings." Frontiers in plant science 10 (2019). PubMed...
Showing: 181 - 200 of 824 results