We narrowed to 821 results for: EED

...understand the principles of gating, you’ll first need to learn a little bit of theoretical background ...software will allow you to adjust this setting as needed. To figure out the appropriate voltages to observe... through maximum current (H), the time the cell needed to pass through the laser (W) and the area under... current and thereby the signal strength. So, we need to adjust the voltage in the cytometer’s software...interest). Let’s double-click inside our gate and proceed to the next plot! Pro tip! If you only get very...gated on your cells of interest, you will likely need to exclude all duplicate events (two cells stuck...analyzed on their staining signals. For this, you’ll need to gate based on the proportion between H and A ...

..., making it easier to produce the constructs you need. Expression vectors within this kit contain a variety...novel and powerful pyranopyridine derivatives." Proceedings of the National Academy of Sciences 113.13 (2016...terminators in this series are just the tool you need. The plasmids in this portion of the BIOFAB Plasmid...-time observation of metabolite production." Proceedings of the National Academy of Sciences 113.9 (2016...pastoris depending upon your specific protein folding needs. The pCri system has recently been used to study...adaptive evolution of antibiotic resistance." Proceedings of the National Academy of Sciences 109.52 (2012...regulation using the PUF protein family scaffold." Proceedings of the National Academy of Sciences 108.38 (2011...

...correction (about 4%) is needed to see muscle improvement, with only 30% correction needed for normal function...multiple requirements must be met. First, CRISPR would need to be delivered to both cardiac and skeletal muscle... editing occur, the CRISPR components would only need to be expressed for a short period of time, which... mature tissues - no problem, as exon skipping proceeds through the non-homologous end joining (NHEJ) ...therapy closer to the clinic, much work is still needed. First, CRISPR delivery must be optimized to: 1...obstacles remain for CRISPR gene editing, but given the speed at which the technology is advancing, precision ...

...redesigned our website to make it easier to find what you need and to bring it in line with current Web Content...global scientific community. As we enter 2025, your feedback continues to guide our innovation.  In response...search experience so you can find exactly what you need, faster. We’re also building a portal to easily ...landscape is evolving rapidly, and with it, the needs of researchers worldwide. Through our growing community...we're equipping every scientist with the tools they need to push the boundaries of discovery. As I look ahead... E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman, L. P., Ferrara, F., Bradbury, A. R. M., Cable...

... whose infection has failed all #antibiotics. We need lytic non-lysogenic phage URGENTLY to find suitable...help clinicians and scientists quickly find much-needed phages for patients.   A phage directory is crucial...chance that an early request for phage may not be needed if other antibiotics work. “We don’t want to cry...phage community until we have someone that really needs it, but then it might to be too late,” Sacher says...treatment occurs. In addition to providing much needed information and potential for successful phage ...phage therapy, Sacher and Zheng also saw the need to archive phage reagents. If a phage researcher retires...

...waiting multiple days for a reply to an email. You need answers: the right ones, quickly, and this is why... to make our support team available whenever you need them, but sometimes that’s not enough. With your...and make a phone call or write out an email. You need help right at the moment you’re looking for it, ...Center to make sure that, whatever your support needs are, we’ve got you covered! In addition to the Help...making it easier than ever to get the support you need. If for some reason you can’t find the answer that... the support we provide based on your valuable feedback. This is Addgene after all, and making your research...

...: Validation post. The primary antibody control needs only to be run once for each primary antibody that...that will be used (Burry 2011). It does not need to be repeated for each experimental run as long as the...addition to the above-mentioned safeguards, you’ll need to test the secondary antibody in the absence of...detected, the level of noise will determine whether it needs to be addressed. In order to correctly interpret...interpret your results, the signal from your target needs to be significantly higher than the background noise...antibodies in the stain. In situations like this, you may need to find alternative primary or secondary antibodies...samples in different lanes in a western blot, you’ll need to include a loading control. A loading control ...

...audience can relate to. It doesn’t need to be anything fancy; it just needs to capture your audience’s attention...competing desires or (3) the presence of obstacles that need to be overcome. Conflict, and its inherent tension...(nonfiction) story. For there to be a story, you need to have conflict. There are a lot of ways to introduce...the Conflict  Once you have your conflict, you’ll need two other things to complete your story: an introduction...listen or watch) further.  Second, the introduction needs to provide enough information so that the audience...

...them You don’t need to explain as much of your science in a weekly lab meeting as you’ll need to for an outsider...telling it. End at the end A story needs a good start point and it needs a good end point. One thematically... a Good Story Start at the start Any good story needs a solid point to kick off at. Whatever you’re working... outsider. And you won’t need to dive as deep into your research at an outreach event as you might in ...

...101: Control Plasmids Need help thinking about the types of controls you’ll need for your next experiment...posts aim to give researchers the background they need to get started using plasmids and the revolutionary...Scientists: Why and Where Do I Find One? If you need help making a transition into a new job or maybe... CRISPR/Cas9 to make a change to the genome, you need to check whether you’ve made the right one. In this...us through some common controls and why you might need them. Happy Reading!   Resources on the Addgene...

...system, but the available reagents do not match your needs. If YFG is thrillingly new, you might have to make... germline. To begin this process, you will first need to do some molecular cloning. Depending on whether...trying to overexpress or knockdown a gene, you might need different plasmids. Harvard’s DRSC/TRiP Functional...different fly lines. Also, if there is a chance you need to combine multiple transgenes in one fly, having...express multiple transgenes, remember that you’ll need to integrate them all at different loci. Fortunately...for use? Random insertions take longer because you need to determine in which chromosome your transgene ...

...quickly learned that my approach needed to adapt based on individual needs. For instance, I had one colleague...shift and navigate the pitfalls? Here's what you need to know.   Figure 1: Management can seem ...steps to a lab assistant. However, I recognized the need to shift my mindset to empower my team and allow...highly self-motivated and excelled when given the freedom to explore projects autonomously. Failing to recognize...managers fail to set expectations or provide timely feedback, leading to unproductive dynamics. I experienced...

...example, I used to constantly underestimate time needed to prep for an experiment (labeling tubes never...after running a protocol a few times, you no longer need the full checklist. Great! But don’t just stop using...into one. Soon you’ll figure out what prompts you need to consistently and accurately run a protocol.   ...they use them.    Prevent Interruptions If you need to focus, a large set of headphones can act as a...wall of pipette tip boxes when she particularly needed to focus, which was extremely effective. Others...at the bench. Don't be afraid to ask for what you need to be successful! Slowing Down At the suggestion...

...means the mentee needs to be open to change and constructive suggestions and feedback. The best thing ... able to: Help you identify specific skills you need for your current position Understand your interpersonal...of the people you work with Give more informed feedback (for example, they may be able to hear you present...elsewhere is able to: Help identify the skills you will need to make a change Provide perspective on core values...at another company/department Give more candid feedback and be able to discuss more confidential topics...

... have its limitations or that other technologies need to be discarded but clearly we are in an era of ...focuses primarily on behavior, you don’t necessarily need to use electrophysiology to prove that your optogenetic...required. In any given optogenetics experiment you may need to inject virus into 20 or more mice and the injections... die injection prior to using the virus. You may need to adjust coordinates based on your own method of... limited by the mouse line itself. Generation, breeding, and maintenance of a mouse line is also an expensive...terminal also affects the length of time you may need to allow the virus to express. For example, if you...surgeries and viral expression, you are ready to proceed to the material science part of optogenetics. In...

...stabilized promoters incorporate feedback loops such as the incoherent feedforward loop (iFFL). In general, iFFLs...to interpret or can interfere with the precision needed in metabolic engineering or other synthetic biology...deoxychromoviridans requires three genes whose proteins are needed sequentially. When Segall-Shapiro et al. placed... In cases where a specific ratio of products is needed, such as within a multi-protein complex, stabilized...between the different components. When your pathway needs precise fine-tuning of gene expression levels, give...

...Fab fragment, it either needs to be directly conjugated to a reporter or you need a light-chain specific...antibody. Fab fragments are useful when you just need an antibody’s antigen binding function but not the...antibodies, you can make your own antibody fragments as needed.  In contrast, anti-IgG antibodies, commonly used...fragments make good secondaries for cases where you need to pre-form primary-secondary antibody complexes...about what pieces of the antibody you do and don’t need, and how the protein will interact with other components...

...primers, or mammalian selection markers. You don’t need a fancy database to organize your own plasmid collection...of your lab can access and use the materials they need, and avoid duplicating your effort. In addition,... it will be easy to find all of the information needed for the process. Always sequence verify Once the...sometimes more depending on the number of features we need to verify. Determining which features to verify ...Remember, when designing a sequencing primer, you need some space between your primer binding site and ...high-throughput plasmid sequencing If you’re like us, you need a strategy for processing several sequencing reactions...

...deposit process. To deposit plasmids online, you will need to create an account with Addgene before starting...depositing 10 or more plasmids. (If you believe you need to deposit a collection that is greater than 75 ...contains columns that correspond to the plasmid data we need; you can copy and paste your information directly...antibiotics, etc.) and stored as glycerol stocks We will need information on how to verify any genomic alterations...modify the gene/insert and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to ...entry fields to describe the next insert. Repeat as needed for more inserts. When you are finished entering...addgene.org. Include your deposit number and which data needs to be updated. When your plasmids arrive at Addgene...

...many cases where the specific viral particles you need are not available, or the cost of custom viruses...too high for your budget. In those cases, you’ll need to produce your viruses in-house.   Introducing...trying to make. For example, bacteriophages will need to be produced in bacteria cells. However, some ... producing a stock P1 virus, so that if you ever need to remake the same virus, you can skip the first... just two days, but add additional costs and may need to be optimized for your purpose. Making your decision... Baculovirus; can produce AAV and lentivirus if needed # of plasmids Three or four Two Packaging limit...