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We narrowed to 224 results for: ENDO 2

Showing: 181 - 200 of 224 results

  1. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    ...establishing the dynamic range of your FRET assay (Figure 2). These labeling methods are freely interchangeable...PMID: 20161833. PubMed Central PMCID: PMC2802762. 2. Dumas, Anaëlle, et al. "Designing logical codon reassignment–Expanding...2009). However, these reagents can bind numerous endogenous biomolecules in cells nonspecifically, and they...

  2. Improving Transduction Efficiency with Exosome AAVs

    Type
    Blog Post
    ... enrich the microvesicles containing AAVs (Figure 2). Since the development of the exo-AAV isolation protocol...useful for therapeutic applications in vivo where endogenous anti-AAV antibodies often neutralize the therapeutic...

  3. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    ...work with Grows extremely fast (doubling time of ~ 2 hours) Amenable to genetic manipulation The first...cinch. This is because it doesn’t secrete its own endogenous proteins, making the recombinant protein its ...

  6. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    ...PubMed PMID: 3459152. PubMed Central PMCID: PMC323600. 2. Chester, Nicholas, and Daniel R. Marshak. "Dimethyl...Tailored mutations are commonly introduced to endogeneous DNA through homology-directed repair (HDR) of...editing requires a template of high homology to the endogenous target, yet to facilitate the repair, the template...template is removed using a methylation-dependent endonuclease (i.e. DpnI), and bacteria are transformed with...

  7. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    ...improve the brightness of previous Nano-lanterns by 2-4 fold and permit five-color fluorescent imaging! ...also envision eNLs as good reporter genes for endogenous protein fusions created using CRISPR/Cas9. eNLs...

  8. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...and open a new conversation about GMOs.  Challenge #2: Technical obstacles to plant synthetic biology In...the IP landscape, using plant parts which are endogenous to the plant, off patent, or not patented, and...

  9. Design Tips for Prime Editing

    Type
    Blog Post
    ...inhibit editing (Anzalone et al., 2019). Figure 2: Starting lengths for optimizing your pegRNA primer...pegRNAs (but not epegRNAs) can improve either endogenous or fused La binding (Yan et al., 2024). ...Adamson, B. (2024). Improving prime editing with an endogenous small RNA-binding protein. Nature, 628(8008),...

  10. Hot Plasmids - February 2022

    Type
    Blog Post
    ...acceptable variations in DR sequences. Figure 2: comparison of Cas 9 vs. Cas 12a expression cassettes...levels of gene activation for both reporter and endogenous genes. It is highly specific in mammalian cells...

  11. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...Era of Personalized Medicine. Genes Dis. 2017 Jun;4(2):43-63. DOI: 10.1016/j.gendis.2017.04.001 Maetzig... that can replicate and divide along with the endogenous DNA. AAV also has low immunogenicity, meaning...

  12. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ...3570-5. doi: 10.1073/pnas.1420294112. Epub 2015 Mar 2. PubMed. Find the plasmids from this publication ...CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system. Xie K, Minkenberg B, ...

  13. Summer SciComm: Preprints

    Type
    Blog Post
    ... scientific communication ecosystem Figure 2: The scientific ecosystem, enriched by open access...providing a public record of changes, critiques or endorsements. In contrast, journal-based peer review is usually...

  14. Harnessing Bacterial Toxins for Allelic Exchange

    Type
    Blog Post
    ...two visible blue or magenta chromoproteins (Figure 2) (Liljeruhm et al., 2018). This system is a nice confirmation... rely on exogenous enzymes and those that use endogenous homologous recombination. Modifying bacterial...and homologous recombination As an alternative, endogenous homologous recombination enzymes can also accomplish... To allow manipulation of bacteria without an endogenous rhamnose regulon, we inserted a copy of rhaS ...

  15. Fluorescent Proteins 101: GFP Fusion Proteins - Making the Right Connection

    Type
    Blog Post
    ..., whereas the mVenus-APT1 fusion does not (figure 2). In the mVenus-APT1, a lipidation motif, which is...assays are qualitative. The Organized Smooth Endoplasmic Reticulum (OSER) assay (Costantini et al., 2012...measures homodimerization of FPs targeted to the endoplasmid reticulum based on the appearance of OSER whorls...CRISPR/Cas mediated HDR) enables the tagging of endogenous genes close to native expression levels. However...

  17. Hot Plasmids - October 2020

    Type
    Blog Post
    ...products have distinct localizations. Figure 2: Split-wrmScarlet labeling proteins with different...expressed in the neuron, the FingRs bind to the endogenous synaptic proteins allowing them to localize to...

  18. CRISPR 101: Any Base Transversion Editors

    Type
    Blog Post
    ...of concept for further development. Figure 2: Mechanisms of deaminase-based adenine transversion...abasic (empty) DNA site using either fused or endogenous Uracil DNA N-glycosylase (UNG) enzymes. Various...efficiency, but it didn’t help: it seemed that the endogenous enzyme is not efficient enough to hinder adenine...

  19. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    ...become a valuable asset yourself.     Fig 2: A diagram of the Thomas-Kilmann Conflict Model, courtesy...makes the teller feel heard. I am not always a stupendous listener, but I aspire to  be. If you don’t at...conflicts within a given organization, it can help tremendously to have as much context as possible. When I ...

  20. A Control for All Seasons

    Type
    Blog Post
    ...as the protocol remains the same.    Figure 2: Microscopy-based antibody experiments should include...albumin. Secondary antibodies can also react with endogenous antibodies naturally present in the sample, for...experimental run (Burry 2011). Autofluorescence Endogenous fluorescence of the sample, or autofluorescence...
Showing: 181 - 200 of 224 results