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  1. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of interest ...light to generate CRY2-CIB1 oligomers that trap the fusion protein away from its usual location, preventing...

  3. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    ...readout. A common method of labeling proteins is to fuse them with a fluorescent reporter, such as GFP or... a transduction reporter. Note that eGFP is not fused to CD45, but is separately expressed. (The reason...

  4. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    ...must wait for the opsin to express and passively diffuse down the length of the fiber before stimulation...are not actively transported and must passively diffuse so, depending on the length of your projection,...

  8. CRISPR Cheat Sheet

    Type
    Blog Post
    ...nucleases that cut or modify RNA as opposed to DNA. Fusing the nuclease to an adenosine deaminase can convert...

  11. Editor's Choice, August 2016

    Type
    Blog Post
    ...catalytically inactive CRISPR endonuclease (dCas9) fused to a deaminase to make the transitions at target...

  20. Build Your CRISPR Vocabulary

    Type
    Blog Post
    ... it have a lot of parts! If you’re still a bit confused by CRISPR acronyms, this post is for you. We’ll...
Showing: 181 - 200 of 365 results