We narrowed to 319 results for: INO

1. SpyLigase Irreversibly Locks Peptides Together for Efficient Cell Capture

   Type

   Blog Post

   ... encoded way. SpyTag is nice and small, just 13 amino acids, but SpyCatcher is a protein. You can fuse...

2. Plasmids 101: Walkthrough of Addgene’s Snapgene-Powered Quality Control Process

   Type

   Blog Post

   .... By easily using BLAST to check the translated amino acid sequence from within Snapgene, we can confirm...

3. Bringing Sustainable Practices to the Lab: Recycling

   Type

   Blog Post

   ... Despite the fact that researchers represent a minority of the world’s population, we account for an estimated...

4. Fluorescent Proteins 101: Fluorescent Biosensors

   Type

   Blog Post

   ...measure the concentration of small molecules like arabinose or hydrogen peroxide or ions like calcium and ...

5. Tips for Improving Your Next Manuscript

   Type

   Blog Post

   ... He works with gene expression in Pseudomonas aeruginosa. Follow him on twitter @100percentSDS.     Additional...

6. Build Your CRISPR Vocabulary

   Type

   Blog Post

   ...this post is for you. We’ll cover the common terminology for the proteins, DNA, and RNA used in CRISPR...

7. Hot Plasmids - October 2022

   Type

   Blog Post

   ...because of the title!) The Myc-tag is a string of 10 amino acids, EQKLISEEDL, that can be incorporated into...

8. The Stingy Scientist: How the Baby Gel Box Was Born

   Type

   Blog Post

   ...not to stick your fingers in during a run or risk minor electrocution.  These days you can buy small gel...

9. Hot Plasmids June 2018 - Reverse transcriptase, nanobody, and protein-DNA interaction tools

   Type

   Blog Post

   ...biotin–phenol and H2O2, APEX2 labels electron-rich amino acid side chains (e.g. Tyrosine) with biotin. These...

10. HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides

    Type

    Blog Post

    ... translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by the ribosome...

11. Summer SciComm Series: Master of SciComm

    Type

    Blog Post

    ...once again write for fun eventually saw an English minor tacked onto my BSc. With this decision I had unwittingly...

12. RUBY-Red Siliques

    Type

    Blog Post

    ...a single mRNA using 2a sites. These convert the amino acid tyrosine to betanin, a member of a class of...

13. Viral Vectors 101: What is a Virus (and a Viral Vector)?

    Type

    Blog Post

    ...talking about viruses, here’s a quick tip on terminology. “Virus” refers to a single type of virus, not...

14. Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring

    Type

    Blog Post

    ...active up to 100°C.  The IgnaviCas9 protein is 1,241 amino acids long, has optimal sgRNA spacer length of 22...

15. Comparing Cas9 to NgAgo: Can the Argonautes Best CRISPR?

    Type

    Blog Post

    ...affect editing efficiency. Smaller size At only 887 amino acids in length, NgAgo is shorter than SpCas9 (1368...

16. Plasmids 101: A Brief History of Plasmids and an Improved eBook!

    Type

    Blog Post

    ...into the E. coli chromosome in some cases. This terminology held until the 1960s when scientists began to...

17. Plant Plasmids and Resources

    Type

    Collection

    ...new window) for Targeted Gene Mutation in Rice - Yinong Yang. The MoClo CRISPR/Cas Toolkit for Plants includes...

18. p53 Pathway

    Type

    Collection

    ... Serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1 PERP TP53...

19. CRISPR Plasmids - Bacteria

    Type

    Collection

    ... gRNA. Adenine base editors convert adenine to inosine, which is replaced by guanosine to create A->G ...

20. New and Upcoming Viral Vectors - September 2019

    Type

    Blog Post

    ...(105322-AAV1) GABA sensors Sensing levels of g-Amino butyric acid (GABA) - the primary inhibitory neurotransmitter...