We narrowed to 280 results for: LUM;

1. Pathways Over Time Plasmids Engage Students in Functional Genomics Research

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   Blog Post

   ...research course that is part of the biology core curriculum at Boston College. Students in the course work...

2. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

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   Blog Post

   ...the group was able to demonstrate that while illumination of neighboring cells evokes action potentials...

3. CRISPR 101: Targeting Non-Coding RNAs with CRISPR/Cas9

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   Blog Post

   ...Noncoding RNAs. Annual Review of Biochemistry. 2012;81(Volume 81, 2012):145-166. doi:10.1146/annurev-biochem-...

4. Hot Plasmids February 2024

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   Blog Post

   ...mitochondrial membranes imaged with structured illumination microscopy in HeLa cells expressing COX8a-mStayGold...

5. Lab Automation at Addgene

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   Blog Post

   ...pipette with how much total sample you need and the volume of the smaller aliquots you’d like to dispense ...

6. Plasmids for Endogenous Gene Tagging in Human Cells

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   Blog Post

   ...tagging using CRISPR/Cas9 in human stem cells to illuminate cell organization." bioRxiv (2017): 123042. Additional...

7. Tips for Titering Your Lentiviral Preps

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   Blog Post

   ...Additional considerations for titering lentivirus Volume and transduction time will influence lentiviral...

8. Experimenting in an Open Source lab: from CRISPR to Cats

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   Blog Post

   ...equipment for new uses. We’ll probably never use the Illumina GAIIx we recently acquired  for sequencing again...

9. Molecular Biology Reference

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   Guide

   ...acquire NGS but one of the most commonly used is the Illumina NGS platform. This is the platform used by Addgene...sequencing partner, seqWell . The actual process of Illumina NGS is not that different from Sanger sequencing...modified fluorescently tagged nucleotides. During illumina NGS, a long piece of DNA is first fragmented into...regular base and thus do not halt the reaction. Illumina NGS, therefore does not require any “normal” bases...

10. Hot Plasmids: Winter 2025

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    Blog Post

    ...negative-going GEVI that can be used in different illumination regimes with fast activation kinetics and high...

11. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...for downstream NGS and means you can use smaller volumes of your library for each experiment. Additional...

12. Production of Virus in Insect Versus Mammalian Cells

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    Blog Post

    ...virus. This P2 virus can be produced in a greater volume and will have a higher titer than a P1 virus. Pro-tip...

13. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...., & Kusamoto, T. (2023) Single-Molecule Magnetoluminescence from a Spatially Confined Persistent Diradical...

14. 3D Printing Meets CRISPR Cas9

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    Blog Post

    ... is docked on the membrane of the endoplasmic reticulum. And graduate students, momentarily forgetting...

15. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ... will die. If the solution is too cold, it will clump prematurely, and not spread out onto the plate. ...

16. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

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    Blog Post

    ...will be very light in coloration and can look very plump. On the side of their abdomen will be a dark spot...

17. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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    Blog Post

    ...secreted proteins are produced in the endoplasmid reticulum, modified in the golgi and then transported in...

18. Truncated gRNAs for Regulating Gene Expression

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    Blog Post

    ...Addgene Scientific Advisory Board. His laboratory at Columbia University is focused advancing scientific discovery...

19. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

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    Blog Post

    ... Boole’s logic shows up in mechanical systems, plumbing, computers, and now through BIL gates we've implemented...

20. A Guide to Starting Your Own Journal Club

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    Blog Post

    ...Perhaps interest has simply waned. When attendance slumps below a certain level or interest wanes off such...