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We narrowed to 525 results for: MEA

Showing: 181 - 200 of 525 results

  1. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    ... yellow GECIs, OCaMP shows: Higher ΔF/F0 (the measure of signal change during calcium events), Greater...curves for OCaMP and various red and yellow GECIs measured with soluble protein. Reproduced from Aggarwal...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...generally have moderate to high immunogenicity, meaning they induce an immune response in the host. So...endogenous DNA. AAV also has low immunogenicity, meaning it does not induce the same high immune response...

  3. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    ...conventional journal. Publishing via open access means that your manuscript will be available to anyone... for publication and is, in most cases, blind (meaning you don’t know who the reviewers are). If the reviewers...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    ...yeast-specific origin of replication (ORI) and a means of selection in yeast cells, in addition to the ...some drawbacks to using auxotrophic markers as a means of selection: A specific selection marker needs...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...lagged behind, likely due to limitations of current means to detect RNA-protein interactions. To address this... E. coli’s BirA*. Faster labeling kinetics also means that BASU could potentially be used in vivo to study...

  6. dTAG - You're it!

    Type
    Blog Post
    ... are happy to share aliquots of dTAG-13 in the meantime, please address your requests for dTAG molecules...the dTAG lentiviral plasmids can serve as a rapid means of comparing functionality of N- and C-terminal ...

  9. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...ill-defined. In this article, I try to clarify what people mean when they ask about “problem solving skills” and...because I can resolve issues effectively does not mean I am qualified to be an astronaut. But, knowing ...

  10. Addgene: The First Twenty Years

    Type
    Blog Post
    ...designing a website users could easily navigate. This meant we could grow accessibility alongside our repository...beginners” on r/labrats, Reddit.  Part of that work meant facilitating the Material Transfer Agreement process...

  11. Viral Vectors 101: An Introduction to AAV

    Type
    Blog Post
    ... used in research have their genomes “gutted”, meaning that all viral genes except ITRs (inverted terminal...capacity Empty rAAV has a cargo capacity of ~ 4.7 kb, meaning that your gene of interest (or genes) need to be...

  12. The time and cost required to make a plasmid

    Type
    Blog Post
    ...put scientific inflation at… roughly 34.9%, which means that the same plasmid would cost $155.25 in reagents...changes. Fun fact: Addgene’s high-throughput approach means we virtually always use every single well in a 96...

  13. To Each HIS Own

    Type
    Blog Post
    ...protein of interest. The tag was developed as a means to purify a protein based on the affinity of the...losing your protein of interest. But that doesn’t mean longer is always better - the longer the tag, the...

  14. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...single sentence and figure must be clear, concise, meaningful, and supportive of the mission of the application...of ‘to be’ verbs creates strong, well-directed meaning and delivers strength to the sentence. Below are...

  17. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    ...strains share a common genetic background, which means that each mouse is genetically identical and all...maximum genetic heterozygosity in the population, meaning they more closely mimic the genetic diversity of...

  19. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... genome, the virus is replication incompetent, meaning it can’t replicate and continue the infection cycle... (or BSL-2+) lab and require additional safety measures.   General viral handling safety   Containment...

  20. 6 Tips for Grant Writing

    Type
    Blog Post
    ...this does not mean that you should apply for everything that’s out there, it just means that you shouldn...
Showing: 181 - 200 of 525 results