We narrowed to 359 results for: Nov
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TypeBlog Post...capable of inducing the brightest fluorescence (Filonov et al., 2014). Broccoli is twice as bright as Spinach2...9.10 (2014): 2412-2420. PubMed PMID: 25101481. Filonov, Grigory S., et al. "Broccoli: rapid selection ...
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Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog Post...adeno-associated virus, herpesviruses, hepatitis B virus, adenoviruses, and human papillomavirus (HPV), to name a few...though the rate of loss greatly depends on cell turnover. Why use integrase-deficient lentiviral vectors... -
Fluorescent Proteins 101: When GFP lets you down
TypeBlog Post...e.g. mScarlet) or infrared fluorescent proteins (Chernov et al., 2017) may improve detection. Since longer...24360272. PubMed Central PMCID: PMC3918502. 14. Chernov, Konstantin G., et al. "Near-infrared fluorescent... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog Post...are not easily reversible. Late last year, the Akhmanova Lab published a new optogenetic tool for localized...Grigoriev, I., Nijenhuis, W., Kapitein, L. C., & Akhmanova, A. (2022). Opto-katanin, an optogenetic tool ... -
Evolution of Lab Techniques
TypeBlog Post...papers or are brainstorming new technological innovations. Let’s take a look at three historical techniques...past. In the sequencing world, one up and coming innovation is nanopore-based DNA sequencing which reads ... -
Hot Plasmids: Spring 2025
TypeBlog Post...flexibility and speed, so you can design, test, and innovate faster than ever. Plus, we've already taken them...potential. Our partners at the Institute for Protein Innovation (IPI) recently released a set of highly-specific... -
Building Global Connections with the International Mentorship Program USA-EUROPE
TypeBlog Post... can exchange ideas, network, collaborate, and innovate internationally. Why an International Mentorship...Spanish Secretary of Research, Development, and Innovation, D. Carmen Vela, who reiterated the Spanish government... -
CRISPR References and Information
TypeCollection... opens in a new window) Can be used to identify novel gRNA target sites in a single gene, as well as a... -
27 Hot Plasmids from 2016
TypeBlog Post...strategies for combating cancers. To facilitate innovative cancer research, the Reference Reagents Group...robust CRISPR activators We previously reported on a novel Cas9-based activator developed by the Church lab...-X identified both previously characterized and novel mutants. Hess et al. envision CRISPR-X as not only... -
Live and Let Dye: Self-Labeling Protein Tags
TypeBlog Post...substrate-linked PEG, biotin, or magnetic beads. In one innovative example, the Zhang lab used SNAP-tag to decorate...Bulleit, R. F., & Wood, K. V. (2008). HaloTag: A Novel Protein Labeling Technology for Cell Imaging and... -
An “elegans” Approach to Better CRISPR/Cas9 Editing Efficiency
TypeBlog Post...simplifies design of these constructs, allowing novice researchers to quickly and easily start genome ...technology into C. elegans has been a transformative innovation for our community. Developments in mammalian ... -
Typing CRISPR Systems
TypeBlog Post...V., Fedorova, I., Karneyeva, K. A., Artamonova, D. N., & Severinov, K. V. (2021). Type III CRISPR-CAS ... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog Post...sites of infection or damage (Peters et al. 2008, Chtanova et al. 2008, Kamenyeva et al. 2015)? How do blood...18703742. PubMed Central PMCID: PMC2606057. 2. Chtanova, Tatyana, et al. "Dynamics of neutrophil migration... -
Plan Your Experiment
TypeCollection...DSBs): For small nucleotide edits that introduce a novel restriction site. The region of interest is PCR ... -
The Pleiades Promoter Project
TypeCollection...Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S, Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu... -
Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs
TypeBlog Post...example, you were the first person to identify a novel protein found in skin cancer and your continued ...career development firm focused on connecting innovative, entrepreneurial companies with like-minded professionals... -
CRISPR History and Development for Genome Engineering
TypeCollection...Abudayyeh OO, Gootenberg JS, Wu WY, Scott DA, Severinov K, van der Oost J, Zhang F. 2017. Multiplex gene... -
Tetracycline Inducible Expression
TypeCollection...tetracycline-dependent transcriptional activators: novel mutations yield expanded range and sensitivity .... -
Viral Vectors 101: Systemic Capsids
TypeBlog Post...Delivering genes across the blood-brain barrier: LY6A, a novel cellular receptor for AAV-PHP.B capsids. PloS One...Sabeti, P. C. (2023). Systemic administration of novel engineered AAV capsids facilitates enhanced transgene... -
Antibody Guide
TypeCollection...sdAb. sdAbs can have weak signals due to their monovalent nature. Darpins - Darpins are genetically engineered...