We narrowed to 290 results for: Ott

1. Degrading DNA with Cascade-Cas3

   Type

   Blog Post

   ... Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3...

2. The AAVantages of AAV in CRISPR Screens

   Type

   Blog Post

   ...intricacies of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to...

3. Magnetic Control of Proteins: More than a Dream

   Type

   Blog Post

   ...10.1021/jacs.3c01076.  Wheeler, M. A., Smith, C. J., Ottolini, M., Barker, B. S., Purohit, A. M., Grippo, R....

4. Viral Vectors 101: Producing Your rAAV

   Type

   Blog Post

   ..., Conlon, T., Darmon, C., Doria, M., Douar, A., Flotte, T. R., Francis, J. D., … Snyder, R. O. (2010)....

5. Twenty Years of Addgene Sharing: CRISPR

   Type

   Blog Post

   ... data said!  CRISPR is always in style When we plotted CRISPR plasmids requested each year by year of ...

6. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

   Type

   Blog Post

   ...Liu, X., Chen, Y., Zhang, Y., Liu, Y., Liu, N., Botten, G.A., Cao, H., Orkin, S.H., Zhang, M.Q., & Xu,...

7. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   ...Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis...

8. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

   Type

   Blog Post

   ... recipients with an integrated copy of CRISPRi. Bottom: An ICE element containing the CRISPRi components...

9. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

   Type

   Blog Post

   ...that satisfy all requirements. To alleviate this bottleneck, we began building CrispyCrunch in July 2018 ...

10. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

    Type

    Blog Post

    ...Disease and Open Science: An Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

11. 10 Basic tips for mammalian cell culture

    Type

    Blog Post

    ...Finally, avoid sticking pipettes into solution bottles directly, rather decant the solutions into disposable...

12. Cloning Mammalian Cells with the Agarose Method

    Type

    Blog Post

    ...Cas9 knockout efficacy, we have used both Western blotting and PCR, depending on the level of endogenous ...

13. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

    Type

    Blog Post

    ...from Sue Jaspersen (Stowers Institute) and Mark Prescott (Monash University). You can find more plasmids...

14. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

    Type

    Blog Post

    ...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

15. Anatomy of a Plasmid Page at Addgene

    Type

    Blog Post

    ... the arrow down for additional resources at the bottom of the page that help you cite this publication...

16. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type

    Blog Post

    ...lot of protein-protein interaction work and we’ve gotten some tools back from Addgene recently to explore...

17. Chemogenetics Guide

    Type

    Guide

    ...Slaviero AN, Silvagnoli AD, Crespo EL, Schalau R, Gott M, Tree MO, Dunbar GL, Rossignol J, Hochgeschwender...

18. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

    Type

    Blog Post

    ...check that your RNase has not expired (or been forgotten).    Improving read accuracy    In many cases,...

19. An Introduction to Adenovirus

    Type

    Blog Post

    .... Rowe, W. P., Huebner, R. J., Gilmore, L. K., Parrott, R. H., & Ward, T. G. (1953). Isolation of a Cytopathogenic...

20. Don’t FRET: Bimolecular Fluorescence Complementation Makes Visualizing Protein-Protein Interactions Easy

    Type

    Blog Post

    ...and their endogenous counterparts using western blotting. The subcellular localization of BiFC constructs...