We narrowed to 290 results for: Ott;

1. How-to: 5 Steps to a Great Panel Discussion

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   Blog Post

   ... discussions see Guidelines for Great Panels by Scott Kirsner and How to Be a Great Moderator by Guy Kawasaki...

2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

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   Blog Post

   ...on one another to celebrate good news or share a bottle of wine after a rough day, and we have great partners...

3. Cloning Mammalian Cells with the Agarose Method

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   Blog Post

   ...Cas9 knockout efficacy, we have used both Western blotting and PCR, depending on the level of endogenous ...

4. Fluorescent Proteins 101: Visualizing Subcellular Structures & Organelles

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   ...from Sue Jaspersen (Stowers Institute) and Mark Prescott (Monash University). You can find more plasmids...

5. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

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   Blog Post

   ... recipients with an integrated copy of CRISPRi. Bottom: An ICE element containing the CRISPRi components...

6. CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments

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   ...that satisfy all requirements. To alleviate this bottleneck, we began building CrispyCrunch in July 2018 ...

7. 10 Basic tips for mammalian cell culture

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   Blog Post

   ...Finally, avoid sticking pipettes into solution bottles directly, rather decant the solutions into disposable...

8. Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR

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   Blog Post

   ...Liu, X., Chen, Y., Zhang, Y., Liu, Y., Liu, N., Botten, G.A., Cao, H., Orkin, S.H., Zhang, M.Q., & Xu,...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

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   Blog Post

   ...Nanobodies from camelids have gotten a lot of press for being a promising approach for treatment or prophylaxis...

10. Degrading DNA with Cascade-Cas3

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    Blog Post

    ... Naito, Y., Nakada, S., Yamamoto, T., Sano, S., Hotta, A., Takeda, J., & Mashimo, T. (2019). CRISPR-Cas3...

11. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...intricacies of molecular pathways. These screens have gotten larger, easier, and more comprehensive thanks to...

12. Magnetic Control of Proteins: More than a Dream

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    Blog Post

    ...10.1021/jacs.3c01076.  Wheeler, M. A., Smith, C. J., Ottolini, M., Barker, B. S., Purohit, A. M., Grippo, R....

13. Viral Vectors 101: Producing Your rAAV

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    Blog Post

    ..., Conlon, T., Darmon, C., Doria, M., Douar, A., Flotte, T. R., Francis, J. D., … Snyder, R. O. (2010)....

14. Twenty Years of Addgene Sharing: CRISPR

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    Blog Post

    ... data said!  CRISPR is always in style When we plotted CRISPR plasmids requested each year by year of ...

15. Starter guide to induced pluripotent stem cells (iPSCs) part 1:  A renaissance in regenerative medicine

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    Blog Post

    ...Disease and Open Science: An Interview with Darrell Kotton Four Factors that Differentiate the Stem Cell Field...

16. When is a Monomer not a Monomer? The Top Three Ways Your Favorite Fluorescent Protein Oligomerizes in Cells

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    ...did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes of Health...

17. Anatomy of a Plasmid Page at Addgene

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    Blog Post

    ... the arrow down for additional resources at the bottom of the page that help you cite this publication...

18. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

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    Blog Post

    ...lot of protein-protein interaction work and we’ve gotten some tools back from Addgene recently to explore...

19. Negative Can Be Positive: Open AAV Data with Addgene

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    Blog Post

    ... A. M., Su, L., Su, Q., Gao, G., Mueller, C., & Flotte, T. R. (2015). Stability and compatibility of recombinant...

20. Isolating Genomic Regions of Interest with the CRISPR System

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    Blog Post

    ... of many human malignancies. Additionally, the Tapscott Lab identified regulators of DUX4, a transcriptional...