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Showing: 181 - 200 of 436 results

  1. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine...

  2. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    ...adding more terms.  Weighting We determined the relative importance of different bits of information for...shortcuts to specific catalog items as well as related items (for example, a kit that contains a plasmid...

  3. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ... promoter, gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself ... vector. In addition, the pol III promoter is relatively weak, and low expression of gRNAs from these ...

  5. RaPID Detection of RNA-protein Interactions

    Type
    Blog Post
    ...that promote RNA decay and their upregulation is correlated with poor survival in breast cancer patients.... wild-type sequences and that loss of binding correlated with increased serum iron levels seen in HHCS...

  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...structures based on morphology. Autophagosomes are relatively easy to identify: double-membraned structures...comprised of two tandem fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive...

  7. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...Mbp, with the calculated fluorescent distances correlating with the previously established physical map....

  8. Antibodies 101: Fab Fragments

    Type
    Blog Post
    ...The modularity of antibodies means that it is relatively easy to physically divide up the different regions...fragments intended for use as primary antibodies are relatively uncommon. Though, if you want to use Fab fragments...

  9. To Each HIS Own

    Type
    Blog Post
    ...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

  10. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  13. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    ...example, while the PEG precipitation method is relatively inexpensive and does not require an ultracentrifuge...titering. Typically researchers will choose a relatively permissive cell line for titering in order to...

  15. Tips for Technical Support Calls

    Type
    Blog Post
    ...these tips and tricks will apply to any biology-related customer support interaction. Before contacting...support scientists wear many hats at this still relatively small nonprofit. If you do go longer than a few...

  17. Antibodies 101: Choosing the Right Isotype

    Type
    Blog Post
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  18. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...City The conference was located in the modern, relatively new “Science City” area on the outskirts of Guangzhouon...of Georgia studies the CRISPR/Cas system as it relates to its natural role in prokaryotic immunity to ...

  19. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...significant amount of equipment, most of which is relatively cheap). Several protocols have been written outlining...appropriately (tuned for optogenetics). These lasers are relatively cheap and will run you about $2-3,000/laser. ...
Showing: 181 - 200 of 436 results