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Showing: 181 - 200 of 437 results

  1. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    ...which can help determine structure and function relationships or, in more practical applications, allow a ... any of a huge variety of ncAAs to study the relationship between structure and function…if you can get...

  2. Viral Vectors 101: Types of viruses

    Type
    Blog Post
    ...lentivirus and gamma-retrovirus are so closely related, their required components are very similar. While...induce a lower inflammatory response within hosts. Relative to the other viruses discussed in this article...

  3. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    ... GPCRs for chemogenetic studies to probe the relationship between neurons and specific behaviors. Chemogenetics...DREADDs are ideal for chemogenetics as they are relatively insensitive to their endogenous ligand, acetylcholine...

  4. Back to Bacteria: CRISPR gRNA Multiplexing Using tRNAs

    Type
    Blog Post
    ... promoter, gRNA and pol III terminator. These relatively large cassettes (considering the gRNA itself ... vector. In addition, the pol III promoter is relatively weak, and low expression of gRNAs from these ...

  6. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...structures based on morphology. Autophagosomes are relatively easy to identify: double-membraned structures...comprised of two tandem fluorescent proteins: a relatively pH-insensitive RFP variant, DsRed.T3 and a pH-sensitive...

  7. CRISPRainbow and Genome Visualization

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    Blog Post
    ...simultaneously, they could also monitor the spatial relationship of the two genes over time, at a range of distances...Mbp, with the calculated fluorescent distances correlating with the previously established physical map....

  8. Antibodies 101: Fab Fragments

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    Blog Post
    ...The modularity of antibodies means that it is relatively easy to physically divide up the different regions...fragments intended for use as primary antibodies are relatively uncommon. Though, if you want to use Fab fragments...

  9. To Each HIS Own

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    Blog Post
    ...binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins containing...questions about polyhistidine tags. For more antibody-related information, sign up to receive alerts for antibody...

  11. Antibodies 101: Affinity Reagents

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    Blog Post
    ...antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative affinity... These are antibody mimics and are not closely related to antibodies. Instead, they are derived from a...

  12. RaPID Detection of RNA-protein Interactions

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    Blog Post
    ...that promote RNA decay and their upregulation is correlated with poor survival in breast cancer patients.... wild-type sequences and that loss of binding correlated with increased serum iron levels seen in HHCS...

  15. Antibodies 101: Choosing the Right Isotype

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    Blog Post
    ... choose primary antibodies from more distantly related species and/or use narrowly specific secondary ... antibodies raised in different, and distantly related, species, and secondary antibodies raised in the...

  17. Overcoming the Challenges of Lentiviral Production

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    Blog Post
    ...example, while the PEG precipitation method is relatively inexpensive and does not require an ultracentrifuge...titering. Typically researchers will choose a relatively permissive cell line for titering in order to...

  18. Tips for Technical Support Calls

    Type
    Blog Post
    ...these tips and tricks will apply to any biology-related customer support interaction. Before contacting...support scientists wear many hats at this still relatively small nonprofit. If you do go longer than a few...

  19. Reaching out to China: Canton Nucleic Acids Forum (CNAF) 2015

    Type
    Blog Post
    ...City The conference was located in the modern, relatively new “Science City” area on the outskirts of Guangzhouon...of Georgia studies the CRISPR/Cas system as it relates to its natural role in prokaryotic immunity to ...

  20. The Materials Science of Optogenetics Experiments

    Type
    Blog Post
    ...significant amount of equipment, most of which is relatively cheap). Several protocols have been written outlining...appropriately (tuned for optogenetics). These lasers are relatively cheap and will run you about $2-3,000/laser. ...
Showing: 181 - 200 of 437 results