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Showing: 181 - 200 of 1005 results

  1. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...give to your questions. If one of their answers rings true for you, go ahead and give a similar answer... to the hiring manager and others, if you have their emails. You can use notes you took during your interviews...Fielding questions can be hard during this talk since you’ll be sharing your screen and won’t be able ...2016 and then working four more years in academia. During my job hunt, I quickly noticed one of the biggest... and/or small groups, usually starting with the hiring manager. These interviews lasted for 30 minute ...a list of who will be interviewing you, ask the hiring manager or recruiter for this information. I found...presentation to your audience Start by asking the hiring manager or recruiter what presentation format they...

  4. The Importance of a Fun Workplace: Company Culture at Addgene

    Type
    Blog Post
    ...actually has chimes outside of our break room. A ring of the chimes means something fun is going on and...in a unique environment where, in addition to furthering our mission by hard work, we genuinely enjoy ... that you don’t work for Addgene (though we are hiring!) but to encourage everyone to make an effort to... the power of simple word of mouth - be sure to bring up your event in casual conversation to get people...

  5. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    ...camera lens without having to physically rotate the rings around the lens. It allowed me to focus on the subject...constraints given. And there were many constraints during the COVID-19 pandemic. The Pandemic Pivot Back...Even Blugene is practicing social distancing and wearing their mask. But while the camera is here, the operator...chair not watching their actors live on set, but peering at a 7-inch wireless monitor. Wireless video technology...control, and extra hand sanitizer.    Cycle 4: Venturing into the Lab Design: The lab is the beating heart...heart of Addgene. It’s why we exist, storing plasmids, and distributing them to labs that need them. The...projects lined up, some that started production during the middle of lockdown, and some that will spin...

  6. Transferable Skills Guide: Creativity

    Type
    Blog Post
    ...to help students during an interview. When they were instructed to use the three-ring binder as a starting...transferable skills learned while working in the lab and offering advice on areas where you might need some help...creativity is absolutely a transferable skill developed during PhD training. Creativity is required to develop...Whether it’s finding the source of contamination or figuring out why the lab is so cold, we’ve all had to think... need ahead of time and mute your notifications during. (Boyes, 2019) If you’ve taken the time to do this... assure that all participants feel comfortable sharing their ideas? And how do we give people the opportunity... The purpose of this form was to encourage the sharing of ideas, not to give people more work. Identify...

  7. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    ...spent hours in the biosafety cabinet with cloning rings or following labor-intensive limiting dilution protocols...is used for a variety of applications such as measuring target protein expression levels, assessing post-translational...uses 2 or 3 different fluorescent colors each measuring a different target. Flow cytometers however are...methanol, fix and permeabilize in a single step by denaturing proteins and dissolving lipids including those...specialized components that can divert a subset of cells. During FACS, the sample stream oscillates to generate ...be targeted with specific fluorescent antibodies during the flow cytometry experiment and will appear as...of yellow and orange from FITC and that from PE. During compensation the signal arising from a specific...

  8. Pouring LB Agar Plates

    Type
    Protocol
    ... Protocols Pouring LB Agar Plates Pouring LB Agar Plates You may also like......solidifies in the bottle while you’re pouring, you should stop pouring and re-make the gel-mix. If you’re... autoclave tape. The autoclave tape will darken during the autoclave process if your sample has spent ...in the autoclave, you should prepare your plate pouring station: Find an empty section of lab bench with... the flame just to the side of where you’ll be pouring your plates - be sure to leave room for your bottle...appropriate antibiotics, and a section for active pouring. Prepare your antibiotics. Prior to adding your...1000x stock solution. For example: If you’ll be preparing plates with a final concentration of 100 ug/mL...

  9. Fluorescence Titering Assay

    Type
    Protocol
    ... Protocols Fluorescence Titering Assay Fluorescence Titering Assay for Lentivirus You may also...cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and ...consider wells with less than 40% fluorescent cells. Titering methods assume one integration event per cell....

  10. Colony Formation Titering Assay

    Type
    Protocol
    ... Protocols Colony Formation Titering Assay Colony Formation Titering Assay for Lentivirus You may also...luer-lock filter, Corning 431229 10 mL luer-lock syringe, BD BD300912 0.45 μm polyethersulfone filter, Nalgene...added at the time of viral transduction, and not during the cell seeding step. Procedure Before beginning...cells and debris. Lentiviral titer can decrease during cycles of freeze-thaw. If you are freezing and ...

  11. Tips to Make the Most of a Scientific Conference

    Type
    Blog Post
    ...like to go to. During the conference Take notes during talks. If you have questions during the talk, jot...conference depends on what you put into it before, during, and after the meeting. Let’s break it down into...Try to avoid jargon in this initial introduction. During the conference When you meet someone new at a conference... new method that you learned about at the talk, bring it to your next lab meeting. Don’t be afraid to ...for both instances. If your poster material is nearing publication, consider uploading your work to a ...poster) so your visitors can have a DOI to go to. During the conference Arrive at the poster session early...the board and meet your poster session neighbors. During the session, if someone comes up to my poster, ...

  12. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ... from Meiring et al., 2022, under CC-BY 4.0 license. A naturally occurring microtubule-severing protein...cells with physical structure, separate chromosomes during mitosis, support transport of organelles and vesicles...localized microtubule disassembly, Opto-katanin (Meiring et al., 2022), that overcomes many of those limitations...Opto-katanin induces fast, localized microtubule severing. A) Schematic of Opto-katanin constructs and activity...highly localized, rapid and reversible microtubule severing and has very low dark-state activity and toxicity...can be critical when, say, the gene is essential during development and a knockout is not feasible or when... One great way to do this is to use CRY2-CIB1 clustering. Fuse either CRY2 or CIB1 to your protein of ...

  13. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    ...This year, the IMP mentoring program extends its scope to the fields of Engineering, Economics, and Philology...internationalization and there is a lack of expert mentorship during undergraduate and graduate studies. Students also...Mentor Circle Programs at BWH and providing career mentoring to several students from Spain and all over the...in several countries, however programs like IMP, bring closer than ever (although not physically) the ideas...exist outside their immediate frontiers. Moreover, sharing a culture/country of origin with an international...2014-15, University of Valencia): “It is very inspiring to be in contact with mentors who are at a professional...the ability to accept feedback and adapt their mentoring styles to the mentee’s personality and professional...

  14. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    ..., try lowering the voltage and using a longer run time.   Samples can run into empty neighboring lanes... two most common (non-technical) issues with transferring are smaller proteins going completely through...through the membrane or larger proteins not transferring completely onto the membrane. If you’re not sure ...sure if your protein of interest is over-transferring (going completely through your membrane to the other...other side), you can check by using two membranes during your transfer instead of one. If you see signal...both membranes, you know your protein is over-transferring! If you see very little signal, but you know... sample of a large protein, you may be under-transferring.  Membrane pore size Membranes come in different...

  15. Mouse Modeling, Part 2: Breeding and Crossing Mice

    Type
    Blog Post
    ...Kleckner. "Recombination, pairing, and synapsis of homologs during meiosis." Cold Spring Harbor perspectives...generate double-mutant offspring. Crossing mice allows for the production of offspring that have a combination...generate a desired phenotype Instead, genetic engineering should be used to separately introduce each mutation...modification will be passed along to all of the offspring in the F1 generation, your breeding scheme should... two mutations that you want combined in your offspring. Genotyping involves getting a small piece of ...is homozygous for a different mutation, the F1 offspring will be either hemizygous or null at one locus...mice that were bred in your colony, as opposed to ordering control mice from a stock center. This serves ...

  16. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    ...tools available for monitoring how both the brain and visceral organs interact during various behaviors.... This is all an impressive engineering feat, but you’re probably wondering “did it actually work”? The...Neuromodulation During Free Behavior. 2020 42nd Annual International Conference of the IEEE Engineering in Medicine...continuously monitor it. How to address this? Through the pairing of materials scientists (Anikeeva lab) with gut-brain...physically hooked up to invasive, cumbersome systems during experiments – neither ideal for the intrepid researcher... fiber (Park et al., 2019). This provided an engineering road map of sorts, giving an idea of the stiffness...affect their function (the gut twists and contorts during the digestion of food, while brain tissue is relatively...

  17. Tips for Writing a Good Cover Letter

    Type
    Blog Post
    ... professor or hiring manager. Otherwise, use a gender-neutral greeting like “Dear Hiring Manager.” Highlight...If you are job searching, you may be wondering if you need to go to the effort of writing a cover letter...requires ddPCR skills. Your resume lists ddPCR, so the hiring manager knows that you have ddPCR experience. In...assay to answer an experimental question. Now, the hiring manager knows that you can design experiments with...briefly explaining a gap in your job history or sharing relocation plans. They can be skills-based: if ... of the department] at [name of the university].During my thesis work on [phenomenon X], I read several...joining your team as a postdoctoral researcher. Dear Hiring Manager, I am writing to apply for [title of the...

  18. A Deep Dive into BioBuilder

    Type
    Blog Post
    ...of “bringing tomorrow’s science into today’s classrooms,” BioBuilder answers the call by offering a variety...Tamer from BioBuilder, an innovative nonprofit bringing the study of synthetic biology into the hands ... Kuldell from MIT’s Department of Biological Engineering. Built primarily from her 20 years of college... boost interest and understanding around the engineering of biology. Participating schools are provided...BioBuilder inspires students and teachers to apply engineering principles to biological systems. Today, what...BioBuilder module allows students to explore how engineering fields rely on rapid prototyping to develop and...high school teams around the world who combine engineering approaches and scientific know-how to design/...

  19. Binning Singletons: Tackling Conference Networking When You Don’t Know Anyone

    Type
    Blog Post
    ...and scientists - Culturing a scientific network We refer to the process of mentoring within a bin as Horizontal...and more. We are partnering with ASM and are working together with the society to bring an even more valuable...next component of Binning Singletons is when we bring all the bins together at a mixer. We call this Quorum...also sought expertise from many with experience mentoring in STEM, including Addgene’s Joanne Kamens. Expanding...Chicago.  Finding our place in networking and mentoring circles We looked at a lot of approaches scientific...scientific meetings have taken to provide mentoring. For example, Goldschmidt conference, Coastal & Estuarine...historically underrepresented groups. Through such mentoring programs, scientists can build and expand their...

  20. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ... IPI and the Springer lab (Hao et al., 2024). In this study, we, along with the Springer lab, reported...stabilizes the extended open integrin conformation. During embryonic development, extended-open integrins ...development, and the shaping of the extracellular matrix. During immune responses, this same conformation encourages...produced from hybridoma antibodies developed in the Springer lab. Some are rabbit immunoglobulin G1 (IgG1) ... to be developed and defined. According to the Springer lab, a highly specific function-blocking antibody...   Targeting integrins in the lab The Ha and Springer labs recently showed RGD-binding integrins demanded...integrins. The Ha lab — in collaboration with the Springer lab, the Jaumouillé lab, the Waterman lab, and...
Showing: 181 - 200 of 1005 results