We narrowed to 222 results for: Rras

1. Transferable Skills Guide: External Collaborations

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   Blog Post

   ...Melanie, et al. "An evidence-based evaluation of transferrable skills and job satisfaction for science PhDs...

2. CRISPR-based Genome Editing Systems For Editing in E. coli

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   ...customized as well, including concatenations into crRNA arrays targeting multiple different sites in E. coli. ...

3. CRISPR 101: Targeting RNA with Cas13a (C2c2)

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   Blog Post

   ...using Cas1, a gene commonly associated with CRISPR arrays and involved in spacer acquisition following infection...

4. Fluorescent Proteins 101: Aptamer Fluorophores

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   Blog Post

   ...important component of high throughput assays with microarrays and biochips. Ultimately, there are just some...

5. New Tools Enable CRISPRa for Neuroscience Applications

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   ...complex genetic programs specify and assemble diverse arrays of neurons, forming the neuronal circuits that ...

6. Plasmids 101: Plasmid Incompatibility

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   ....2015.03.007 Pubmed PMID: 25889267 Ebersbach G, Sherratt DJ, Gerdes K (2005) Partition-associated incompatibility...

7. Healthcare Consulting: A Door to the Business of Life Sciences

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   Blog Post

   ...and synthesizing this knowledge into a coherent narrative that gives the client a defined path forward. ...

8. CRISPR Activation: A Practical Guide

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   Blog Post

   ... the promoter of another gene, thereby driving aberrant transcription. That being said, we generally pick...

9. Getting the Most from Your Lentiviral Transduction

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   Blog Post

   ...G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.” Biotechniques 35.4...

10. Twenty Years of Sharing: Addgene's Viral Vector Service

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    Blog Post

    ...robust high quality viral vector service, making an array of ready-to-use viral vectors available to researchers...

11. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

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    Blog Post

    ... single cell with all its synapses and how it rearranges these synapses in the living brain over time ...

12. Data Freedom: The Expansion of Data Sharing in Research Publications

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    Blog Post

    ...supporting datasets must be logically annotated and arranged, as in an actual publication and not require interaction...

13. Adeno-associated Viruses (AAVs) for Genome Editing

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    Blog Post

    ...non-sequence specific frameshift mutations through aberrant double strand break repair, it has proven more...

14. Antibodies 101: Introduction to Immunofluorescence

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    Blog Post

    ...target common IgGs and then conjugate them with an array of fluorophores. It is common for labs to keep a...

15. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

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    Blog Post

    ...Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk R, Auricchio A (2013) Effective ...

16. Using Phosphoserine to Study Protein Phosphorylation

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    Blog Post

    ...expansion of studies on protein phosphorylation in an array of model systems coupled with advances in mass spectrometry...

17. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

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    Blog Post

    ...functional Cas9 nuclease, so there’s no potential for aberrant DNA cleavage. Direct HIV-1 cleavage allows T-cells...

18. CRISPR 101: Homology Directed Repair

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    Blog Post

    ...repair and their potential to induce chromosomal aberrations. Pfeiffer P, et al. Mutagenesis (2000). PubMed...

19. An Integrin Antibody Toolkit from IPI

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    Blog Post

    ... the human body, integrins are key to a diverse array of processes, including cell adhesion, migration...

20. Thoughts on the Future of Research 2015 Boston Symposium

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    Blog Post

    ...the training they receive as postdocs is not transferrable to other environments. Slide from Paula Stephan...