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We narrowed to 613 results for: SET

Showing: 181 - 200 of 613 results

  1. AAV ddPCR Titration

    Type
    Protocol
    ...multichannel pipette set to 5 µL. For dilutions 6–8, use the 20–200 µL multichannel pipette set to 50 µL. For...dilutions, use the 20–200 µL multichannel pipette set to 90 µL and mix by pipetting the liquid up and down...ddPCR plate onto a chilled 96-well freezer block and set aside in the droplet generation BSC to cool. Prepare... Droplet Reader. Open the QuantaSoft software to set up a new plate layout. Designate the sample name,...

  3. Transferable Skills Guide: Time Management

    Type
    Blog Post
    ...variety of transferable skills. At Addgene we've set out to fill this gap by both highlighting that researchers...-day experiment in the lab. You have two ways to set up the experiment. The first way, it will take you... project (with metrics if possible) and, at the outset of a task, I outline the activities that are needed...

  4. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    ...are ready to begin the screening process. Want to set yourself up for a screening success? Before you start...look for off-target changes. This method requires a set of control cells to compare the sequencing reads ...

  5. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    ...from someone seeing me speak in a non-interview setting. There are many resources on self-promotion (how...of this article will be talks for non-academic settings. Many of these guidelines will apply to any type...

  6. Tips for CRISPR Gene Editing in Mice

    Type
    Blog Post
    ...contamination with RNase leading to degradation that can set you back days. Working with plasmid DNA is much easier...pCAG-EGXXFP plasmid? They are the perfect primer sets to use to genotype your pups. A simple PCR with ...contains humanized Cas9 expression cassette and sgRNA expression cassette. The target sgRNA can be cloned...of validation for DSB mediated EGFP expression cassette reconstitution. When the target sequence was digested...took place and reconstituted the EGFP expression cassette. (b) pCAG-EGxxFP target plasmid and pX330-sgRNA...

  8. Fueled by Coffee at #SfN14

    Type
    Blog Post
    ...while keeping the party going! At Addgene, we’ve set-up an exhibitor booth in order to help scientists...

  13. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    ...the biggest hurdles for scientific sharing. So we set out to streamline this process. We take pride in ...

  15. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...features and applications of a partiular plasmid or set of plasmids. We hope that these articles make it ...library that contains up-to-date information and data-sets, as well as an interactive gRNA viewer. The Moffat...address this problem, Csaba Pál’s lab has created a set of vectors that allows one to use the MAGE method...method in unmodified bacterial species (4). This set of plasmids (dubbed pORTMAGE) expresses the λ Red recombinase... kindly constructed and deposited an unpublished set of Zika gene plasmids to Addgene’s repository. The... unique tool with their Yeast Prototrophy Kit, a set of plasmids designed to complement unused auxotrophies...Every quarter we highlight a subset of the new plasmids in the repository through our hot plasmids articles...

  16. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    ...specifically developed to thrive in laboratory settings. One of its most attractive features is its stability...easily maintained in a laboratory environment. What sets Arabidopsis apart from other plant model organisms...

  17. Five Popular Model Organisms

    Type
    Blog Post
    ...in leukemia, the Lowe lab was able to identify a set of mutations that made cancer cells more resistant...systems check out Gerald Rubin’s lab who created a set of modular vectors that can be used to optimally ...

  18. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    ...Goedhart and colleagues in the Gadella Lab devised a set of optogenetic switches for the GEFs ITSN1, TIAM1...controlled phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and...

  19. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    ... go over the details step-by-step. 1. Digestion Set up restriction digests for your insert (or donor ...of recipient plasmid to insert. It is critical to set up a negative control ligation reaction with no insert...

  20. Microbes: Look, Listen, and Tell

    Type
    Blog Post
    ...approaches to visualize bacteria in an educational setting. The Symposium on ‘Creative Approaches to Presenting...
Showing: 181 - 200 of 613 results