We narrowed to 302 results for: SPI

1. Choosing the B(right)est Fluorescent Protein: Photostability

   Type

   Blog Post

   ...photon-excitation, selective plane illumination, TIRF or spinning disk confocal use entirely different excitation...

2. CRISPRainbow and Genome Visualization

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   ...boxB Magenta boxB-MS2 White boxB-MS2-PP7 Despite the success of Ma et al.’s approach, fluorescently...

3. To Each HIS Own

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   ... for nonspecific binding of untagged proteins. Despite being a relatively low frequency amino acid, proteins...

4. Developing Lab Management Software for Biology

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   Blog Post

   ...Python or MATLAB. Choosing Python makes it easy to spin up a read-only Jupyter notebook for them and let...

5. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

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   ...system, including the motor cortex, cerebellum, and spinal cord. Cre-DOG is also suitable for optogenetic ...

6. The Future of Research Symposium Boston 2015

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   ...growing dialogue on the state of a system that, despite all of its successes, has many deep-rooted flaws...

7. Writing Scientific Manuscripts: Literature Searching, Reading, & Organizing

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   Blog Post

   ...Check out the hashtag #365papers on Twitter for inspiration and accountability. There are a few different...

8. Overcoming the Challenges of Lentiviral Production

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   ...ultracentrifugation, centrifugation on a sucrose gradient, spin columns, and polyethylene glycol (PEG) precipitation...

9. Lab Automation at Addgene

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   Blog Post

   ...things your lab can do to get into the robotics spirit include: Start using electronic multichannel and...

10. Science communication: 9 Strategies to Get Your Foot in the Door

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    Blog Post

    ... careers are in advance of yours, but also get inspired and meet peers. Join societies like National Science...

11. Fluorescent Proteins 101: Introduction to FRET

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    Blog Post

    ...measure the tension in the helical structure of spider silk, or the activity of a protein in a synapse...

12. Plasmids 101: The Wide World of Natural Plasmids

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    Blog Post

    ...with a penicillin resistance plasmid can grow in spite of a hostile Penicillium fungus trying to kill them...

13. CRISPR in the Clinic

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    ...currently working on giving this treatment a CRISPR spin. CRISPR CAR-T treatments for leukemia, lymphoma,...

14. Savvy Advocates Needed to Navigate a Scientific Enterprise in Flux

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    Blog Post

    ...for students interested in leading the process despite the recent advance. Similarly, grad students throughout...

15. The Materials Science of Optogenetics Experiments

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    ...science writing. Derek is also a pianist and an aspiring composer. View his blog drsimonsaysscience.org...

16. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

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    ...Editing of Pten by CRISPR/Cas9 in Mouse Liver in Spite of Cas9-Specific Immune Responses. Human Gene Therapy...

17. xCas9: Engineering a CRISPR Variant with PAM Flexibility

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    Blog Post

    ..., resulting in lower overall genome frequency. Despite the progress we’ve made, there is still a need ...

18. AAV Vector Quality Control: Going the Extra Mile with NGS

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    Blog Post

    ...see how low we could go. We prepared a range of spiked samples from 0.1-20% and discovered that, with ...

19. Cloning Mammalian Cells with the Agarose Method

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    Blog Post

    ...dish containing the circled clones is removed by aspiration and the dish left covered (to avoid drying out...

20. The AAVantages of AAV in CRISPR Screens

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    Blog Post

    ...., Garcia, S. P., Zaborowski, M. P., Volak, A., Spirig, S. E., Muller, A., Sousa, A. A., Tsai, S. Q., ...