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We narrowed to 556 results for: SPR

Showing: 181 - 200 of 556 results

  2. Pouring LB Agar Plates

    Type
    Protocol
    ...empty section of lab bench with a working flame. Spray down the bench with a 70% ethanol solution and wipe...

  5. CRISPR Guide

    Type
    Guide
    ...Guide CRISPR Guide Jump To: CRISPR Guide Overview CRISPR Basics Small Edits Large Edits Genome-Wide Screening...increase nuclease activity SpRY - NRN/NYN, where R = A or G, Y = C or T SpRYc - SpRY PAMs plus NNG; a chimeric...Figure 7: Overview of CRISPR transposases Browse Plasmids: CRISPR transposases CRISPR Recombinases and Integrases...screening using CRISPR libraries What Are Pooled Lentiviral CRISPR Libraries? Pooled lentiviral CRISPR libraries...transcriptional repression (CRISPR interference, or CRISPRi) or activation (CRISPRa) of downstream target genes...References Website CRISPR plasmids and resources at Addgene CRISPR 101 eBook (3rd edition) CRISPR software and... Addgene Blog CRISPR topic page How to design your gRNA for CRISPR genome editing CRISPR 101: Homology...

  6. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...PMID: 23328393 Wu lab human lentiviral CRISPR library     CRISPR pooled libraries enable researchers to...Drosophila CRISPR library Addgene has had the privilege of distributing several human and murine CRISPR pooled... Toronto KnockOut (TKO) CRISPR Library. This complex second-generation CRISPR lentiviral library targets...for high-throughput CRISPR/Cas9 gene modification in Caenorhabditis elegans CRISPR/Cas9 technology has...lab overcame this hurdle using CRISPR technology. First, they used CRISPR to create OsTIR1-expressing cell...Find Lourido CRISPR Plasmids. Sidik and Huet, et al. Cell. 2016. PubMed PMID: 27594426 CRISPR-X: dCas9-...mutants. CRISPR-X, a new system from Michael Bassik’s lab, overcomes this obstacle. With CRISPR-X, dCas9...

  7. Degrading DNA with Cascade-Cas3

    Type
    Blog Post
    ... blog CRISPR 101: Cas9 vs. The Other Cas(s) CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems..., these types of CRISPR systems work using a multi-Cas complex called Cascade (CRISPR-associated complex...mechanical work for editing. Class 1 CRISPR systems make up the majority of CRISPR-based immune systems in bacteria... Engineering Using CRISPR-Transposons Additional resources on Addgene.org CRISPR guide Cascade-Cas3 ...The versatility of CRISPR allows you to play with DNA in a number of ways, from small edits that change...derivative of Cas9, but the ever-expanding repertoire of CRISPR has brought many other Cas effectors to light. ... A “complex” system Cas3 is part of the Class 1 CRISPR family, which means it requires multiple Cas effectors...

  8. Your Top Requested Plasmid in 2015!

    Type
    Blog Post
    ...the Addgene repository in 2015 was... lentiCRISPR V2   LentiCRISPR v2 originates from Feng Zhang’s lab... and how does it work? LentiCRISPR v2 purpose As its name suggests, lentiCRISPR v2 is a lentiviral transfer...Genome-wide Screening Using CRISPR/Cas9. If you need help cloning a gRNA into lentiCRISPR v2, you can take a look...on the Addgene Blog Browse Our CRISPR Posts Learn about Using CRISPR to Generate Knockouts Read Some ...with all of the exciting developments surrounding CRISPR genome engineering in the past year, we're excited...,000 human and mouse genes respectively, use lentiCRISPR v2 as the backbone for their many gRNAs. The ...high titer lentivirus production afforded by lentiCRISPR v2 is key to its efficient use in screening experiments...

  9. Adeno-associated Viruses (AAVs) for Genome Editing

    Type
    Blog Post
    ...adeno-cre. AAV-based gene editing compared to CRISPR editing CRISPR, of course, has started a revolution in ...whether to use AAV or CRISPR for a given experimental approach. First, unlike CRISPR, the AAV-based approach...delivering CRISPR components as RNPs and delivering two halves of a repair template spread across two ... editing." Precision Medicine, CRISPR, and Genome Engineering. Springer, Cham, 2017. 29-42. PubMed PMID...in gene editing. However, while CRISPR is remarkably effective for introducing non-sequence specific frameshift...proven more challenging to develop high efficiency CRISPR-based approaches for the generation of knockins...simultaneously in the same cell, are more appropriate for a CRISPR-based approach. Second, the AAV preps generally...

  10. Hot Plasmids - August 2020

    Type
    Blog Post
    ...cells Nanobody purification CRISPRi and CRISPRa system for hPSCs The CRISPR corner New from the viral service...multiplexed CRISPRi and CRISPRa system for human pluripotent stem cells By Jennifer Tsang CRISPR gene interference...interference (CRISPRi) and gene activation (CRISPRa) are great ways to modulate the expression of nearly...57500-1 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...  PAC-MAN (prophylactic antiviral CRISPR in human cells) is a CRISPR-Cas13 based antiviral method to degrade...Addgene The MoClo CRISPR/Cas Toolkit for plants includes 95 plasmids consisting of CRISPR/Cas nucleases, ...

  11. 15 Hot Plasmids from 2017

    Type
    Blog Post
    ...to increase CRISPR editing efficiency across multiple CRISPR enzymes. Plasmids for CRISPR coselection ...podcast segment on CRISPR tools for plants CRISPR inhibitors Bacteria utilize CRISPR Cas9 systems to defend...on Coselection with Ouabain New CRISPR base editors The popular CRISPR base editing technique uses dCas9...of essential genes in vivo. Utilizing CRISPR interference (CRISPRi) technology, the arrayed knockdown library...back using CRISPR inhibitors. Several such inhibitors have been characterized for type I CRISPR systems,... et al. PLoS ONE. 2017. PubMed PMID: 28241009 CRISPR coselection with ouabain increases editing efficiency...Doyon’s lab has developed a new method to increase CRISPR editing efficiency in mammalian cells. Their marker-free...

  12. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    ... DNA Endonuclease Targeted CRISPR Trans Reporter Cas12a (Cpf1) is a CRISPR Cas variant that can also cleave...Resources on Addgene.org Checkout our CRISPR guide page Browse the CRISPR Collection ...In the last few years, scientists have utilized CRISPR-Cas9 protein variants, Cas13, and Cas12a, to develop...develop the method termed DNA Endonuclease Targeted CRISPR Trans Reporter (DETECTR) (Chen et al., 2018). Both...Together SHERLOCK and DETECTR demonstrate the power of CRISPR-based diagnostics. SHERLOCK- Specific High Sensitivity...Abudayyeh et al 2016). Cas13 can be guided by a single CRISPR RNA (crRNA) to cleave ssRNA or mRNA. It also exhibits...reporters for each enzyme. In addition, Csm6, a CRISPR type-III effector nuclease, can be used in conjunction...

  13. Giving gRNAs a Facelift - Synthetic and Beyond

    Type
    Blog Post
    ...design your CRISPR gRNA CRISPR 101: Ribonucleoprotein (RNP) Delivery Components of CRISPR/Cas9 ...sequence for CRISPR-Cas9 targeting is led by a ~20 bp gRNA which is part of the larger CRISPR RNA (crRNA...vectors View Addgene's CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids...surprise then that stability of the RNA used in CRISPR-Cas9 experiments – the “gRNA” – suffers from the...modifications listed above were developed before CRISPR-Cas9, for use in applications like siRNA and antisense...to find pre-tested gRNA sequences for your next CRISPR experiment.    Chemical RNA modifications... On-off switches for gRNAs A major drawback of CRISPR-Cas9 technology is that most expression systems...

  14. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    ...yeast CRISPRa plasmids here Reduce the scale of your CRISPR screens by using this lentiviral CRISPR human...for use in bacteria CRISPR-Casɸ mGold, a new yellow fluorescent protein New CRISPR plasmids New items ...Hypercompact genome editor CRISPR-Casɸ By Melina Fan The Doudna Lab adds to the CRISPR-Cas toolbox with their...sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR plasmids. To...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...their discovery of CRISPR-Casɸ, a hypercompact genome editor from the Biggiephage clade of huge phages. ... B is G, T, or C). The authors demonstrate that CRISPR-Casɸ is active in vitro as well as in human and...

  15. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    ... combine CRISPR and AAVs. 9. CRISPR 101: Validating Your Genome Edit Once you’ve used CRISPR/Cas9 to make...Cpf1: A New Tool for CRISPR Genome Engineering While the vast majority of CRISPR plasmids in our repository...genomic locations. 6. CRISPR 101: Non-homologous End Joining So you know that CRISPR can be used to generate...Recent Plasmids 101, CRISPR 101, and Career Posts Resources at Addgene Browse Our CRISPR Resources Learn ... continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to give researchers...get started using plasmids and the revolutionary CRISPR technology in their own labs. We’re overjoyed to...and happy reading! Top 10 blog posts of 2015 1. CRISPR 101: Homology Directed Repair Addgenie Chari Cortez...

  16. CASTing Off for New Shores in Human Genome Editing

    Type
    Blog Post
    ...and repurposed nuclease-deficient CRISPR-Cas systems to help them spread through bacterial genomes (Peters...Engineering Using CRISPR-Transposons Degrading DNA with Cascade-Cas3 Typing CRISPR Systems Prime Editing... Flexibility to CRISPR Editing Additional resources on Addgene.org Addgene's CRISPR Guide (Jump to Large...hardly seems like we finish writing about one dual CRISPR-transposon system before another exciting new advance...emerges! The programmability and targeting power of CRISPR combined with the large sequence capacity of transposons...arrival on the scene is called evoCAST — an evolved CRISPR-associated transposase (CAST). Back up a second... In either type of CAST, the nuclease-deficient CRISPR-Cas system identifies and binds a genomic target...

  17. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    ...more of these kits, as there are many options! CRISPR CRISPR-Cas can harness the power of MoClo to expand...repurposed for CRISPR applications (gene insert = Cas9 + gRNA), Addgene distributes specialized CRISPR kits too...Enables Rapid Assessment of Emerging CRISPR Technologies. Cold Spring Harbor Laboratory, 27 Oct. 2023. Crossref...including conditional gene expression, multiplexed CRISPR, and chromosomal integration of larger expression...targetability and effectiveness. Perhaps the most intuitive CRISPR MoClo application is to assemble multiple unique...edit optimization, and more. While there are many CRISPR-Cas libraries and tools available for certain model...biologists, Addgene has not one, not two, but three CRISPR MoClo kits for plants that work for both monocots...

  18. A Needle in a Base-Stack: Cas9 Structural Biology

    Type
    Blog Post
    ...Addgene's CRISPR Guide Addgene's CRISPR 101 eBook  Resources on the Addgene blog Components of CRISPR  xCas9...engineered CRISPR enzymes with their own tradeoffs. And this article only covered Cas9! Other CRISPR enzymes...Have you ever designed a CRISPR guide RNA and wondered why it is limited to only 20 bases, or why it’...structure can help us understand the parameters of CRISPR experimental design. Let’s dive into some structural...known proteins; they are completely unique to the CRISPR system (Nishimasu et al., 2014). Table 1. Summary...positively charged channel between the two lobes. For CRISPR to be programmable for any arbitrary sequence, ...better understand how to manipulate it. Happy CRISPRing!   Molecular graphics and analyses performed with...

  19. Hot Plasmids and Viral Preps - July 2021

    Type
    Blog Post
    ...article: CRISPR-based editing in organoid Biosensors for detecting strigolactones The CRISPR Corner New...system, showing the workflow for inducible CRISPR inhibition (CRISPRi). A) Lentiviral vector design. B) Strategy...10.1021/acssynbio.0c00192   The CRISPR corner By Jennifer Tsang New CRISPR plasmids are always being added...To find all of the CRISPR plasmids available from Addgene, head over to our CRISPR Plasmids and Resources...New ready-to-use viral preps New toolkit for CRISPR-based genome editing in organoids By Ashley Waldron..., organoid users have recently begun to develop CRISPR-based approaches for studying gene function in ...derived from other tissue-types. By bringing together CRISPR-based approaches and organoid models, toolkits ...

  20. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    ...Addgene.org Learn All about CRISPR on Our CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments...mammalian genomes as assessed by CRISPR design tools at crispr.mit.edu. They tested their system in HEK293...If you follow CRISPR research, you know all about using non-homologous end-joining (NHEJ) to make deletions...lab has harnessed MMEJ to create a new method for CRISPR gene knock-in, termed PITCh (Precise Integration...percentage of the mutations seen with TALENs and CRISPR. MMEJ is active during M and early S phases, when... PITCh-based genomic insertion would compare to CRISPR sticky-end insertion using the nuclease Cpf1. Since...represents another strategy researchers can exploit for CRISPR gene editing. In organisms where HDR is downregulated...
Showing: 181 - 200 of 556 results