...targeted to specific tissues, cell types or even subcellular regions of a neuron. Optogenetic approaches excel... excel at discrete cell or tissue-subregion targeting down to the limits of light delivery. Chemogenetic...DR If precise spatiotemporal control of a small subset of neurons is necessary, use optogenetics. If broad...

...to silence or incite neuronal firing and study subsequent effects on behavior. The system is an especially...the lab expressed the LiGluR opsin in different subsets of spinal cord neurons using the Gal4/UAS system...blog posts Read blog posts about viral vectors Subscribe to the Addgene blog Resources on Addgene.org ...

...total of eight evolutionarily conserved CRISPR subtypes comprised of more than 40 cas gene families have...grouped the systems into three distinct types and subtypes using polythetic classification based on evolutionary...system and are not translatable to the other CRISPR subtypes. Still, the large and dynamic range of CRISPR ...

...labs. This week we are pleased to announce the submission of the results of our first study which can be...PubMed PMID: 16528756. 2. Oh, Steve KW, et al. "Substantial overproduction of antibodies by applying osmotic...Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal trafficking of adeno-associated...

...interest in STEM subjects; we generally refer to them as the “interested public.” Subscribers to Scientific...Common ones include age range, familiarity with the subject, shared interests, socioeconomic and/or racial/...

... (Fig. 1). However, once thawed, there was a substantial reduction (from 7.4 to 5.0) in the pH of samples...periods, and measured the pH. We found that tubes subjected to a recovery period of at least 96 hours had ...infectivity of the virus. However, there was a substantial reduction in infectivity after 3 weeks of exposure...

...temperature ranges and make them grow better at suboptimal ones, which could allow even your regular E. ...worth the trouble.  Enter ELSA: E. coli Learning Suboptimal Acclimatisation To resolve this impasse, our ...mesophilic chaperones would not function well under suboptimal conditions. This means recombinant proteins can...

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ...amplicons based on sequence homology, and the subsequent product is then digested and ligated into an ...nick repair is needed. Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is...

...ii) embedded in a gel, or (iii) attached to a substrate. These in vitro methods allow for a well-controlled... exchanging excitation filters). This avoids substantial changes in excitation power and will help to ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

...Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be fused to...suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang et al. tested pairs...soon to allow genome modification only in a small subset of cells? Other proteins may also be utliized as...

...cell. Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...viral replication are toxic. Second, infecting subsequently higher and higher order neurons doesn’t reveal...but the newly released RABV cannot infect any subsequent cells due to lack of G protein on the viral envelope...

...recombinant form through DNA sequencing of the line and subsequent cloning of the antibody chains (Crosnier et al... mammalian cells, for antibody production and subsequent purification.  Academic laboratories and companies...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances like...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... were exposed to blue light. Cre-Vvd showed substantially improved activity as compared to other recombinase...fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered prime editors to expand their...

...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

...extracted from purified, DNase-treated AAV and submitted for NGS. Raw sequencing data is analyzed to determine...sequences. Recombinase-dependent sequences may be subject to recombinase-independent recombination during...Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

...expensive exogenously-added substrate, chromoproteins don’t require other substrates for screening. Thus chromoproteins...distinction by name, chromoproteins are actually a subset of the fluorescent protein family. What sets them...

...generated with MXS-chaining were used to label subcellular structures in live cells. Four individual expression...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone ...

... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...number genes may mimic viability genes as they are subject to an increase in dsDNA breaks that can cause cell...al. demonstrated that the Calabrese library substantially identified more hits than the SAM library approach...

... Cas13a binds and cleaves RNA rather than DNA substrates. In terms of structure, Cas13a shares no homology...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ...hundreds of observed shRNA off-targets. They subsequently used catalytically inactive dLwaCas13a as a ...