We narrowed to 579 results for: SUB;

...recombineering to create insertions, deletions, and substitutions - at the same efficiency as Gibson Assembly ...amplicons based on sequence homology, and the subsequent product is then digested and ligated into an ...nick repair is needed. Since the bacteria are subsequently grown at 37 °C, the recombineering plasmid is...

...ii) embedded in a gel, or (iii) attached to a substrate. These in vitro methods allow for a well-controlled... exchanging excitation filters). This avoids substantial changes in excitation power and will help to ...Backbones with Fluorescent Tags Find Plasmids for Subcellular Localization Browse the entire Fluorescent Protein...

...Nanobodies binding GFP were designed in 2009. Subsequent work showed that nanobodies could be fused to...suddenly seemed very realistic. Envisioning GFP as a substrate promoting dimerization, Tang et al. tested pairs...soon to allow genome modification only in a small subset of cells? Other proteins may also be utliized as...

...cell. Once this first order infection occurs, subsequent cycles of viral replication and retrograde transmission...viral replication are toxic. Second, infecting subsequently higher and higher order neurons doesn’t reveal...but the newly released RABV cannot infect any subsequent cells due to lack of G protein on the viral envelope...

...unusual substrates that other organisms can’t use. Other plasmids offer resistance to toxic substances like...possess plasmid-borne toxins like colicins and subtilisins to kill other bacteria in the area, freeing up...

...Every few months we highlight a subset of the new plasmids and viral preps in the repository through ... were exposed to blue light. Cre-Vvd showed substantially improved activity as compared to other recombinase...fluorescent reporter. Find the plasmids here Yongsub Kim’s lab engineered prime editors to expand their...

...recombinant form through DNA sequencing of the line and subsequent cloning of the antibody chains (Crosnier et al... mammalian cells, for antibody production and subsequent purification.  Academic laboratories and companies...Engebrecht J, Trimmer JS (2019) A toolbox of IgG subclass-switched recombinant monoclonal antibodies for...

...check its subcellular location and solubility. If it’s membrane-bound or located in a subcellular compartment...strengths. If that’s not working, consider using a subcellular fractionation kit or a lysing protocol developed...strength Sonication based lysing protocol Subcellular fractional kit or lysing protocol Not enough...

...extracted from purified, DNase-treated AAV and submitted for NGS. Raw sequencing data is analyzed to determine...sequences. Recombinase-dependent sequences may be subject to recombinase-independent recombination during...Check out our Viral Vectors Featured Topic Page Subscribe to Viral Vectors Blog Posts Learn How We Use NGS...

...expensive exogenously-added substrate, chromoproteins don’t require other substrates for screening. Thus chromoproteins...distinction by name, chromoproteins are actually a subset of the fluorescent protein family. What sets them...

... in the depletion of essential genes. In the subsampling analysis of the sgRNAs, Brunello even with only...number genes may mimic viability genes as they are subject to an increase in dsDNA breaks that can cause cell...al. demonstrated that the Calabrese library substantially identified more hits than the SAM library approach...

...generated with MXS-chaining were used to label subcellular structures in live cells. Four individual expression...detection of each fluorophore at the respective subcellular structure (Figure 1). Here, MXS Chaining gave...Excitation/emission maxima Tethering partner Subcellular localization 1 TagBFP 399nm/ 456nm histone ...

... Cas13a binds and cleaves RNA rather than DNA substrates. In terms of structure, Cas13a shares no homology...Cas13a as a diagnostic tool, as discussed below. Subscribe to CRISPR Blog Posts Applications of Cas13a   ...hundreds of observed shRNA off-targets. They subsequently used catalytically inactive dLwaCas13a as a ...

...textbook. Get the journal’s submission rules for review articles Whether submitting a review by invitation ...for their feedback on the scope and the content. Submit your article! Overall, writing a review can be ...

...reporter (Figure 2). The GUS enzyme cleaves a substrate, which will then form a blue precipitate. GUS ...Luciferases are great quantitative reporters and use a substrate to produce bioluminescence. Luciferases usually...fused to a protein of interest to elucidate the subcellular localization of your protein of interest. Find...

...probe gene-function relationships in defined substructures of the CNS as well as individual neuronal populations...demonstrated by the Qi Lab, which developed a subgenome-scale CRISPRa screening approach in embryonic ...transduced with a pooled sgRNA library and were subsequently cultured for nearly two weeks to allow differentiation...

...cytokinesis. Why name this protein after a vegetable substitute for spaghetti? I actually couldn’t find the specific... to segmental clusters of the mesoderm. This subdivision of the mesoderm is what gives rise to different...have severely disrupted visceral mesoderm and subsequently the gut does not form properly (Azplazu and ...

...output of an initial process as the input to a subsequent process, we linked each step in our pipeline ... build one process at a time and connect each subsequent process to the existing ones. Once our first ...as output, which we specified as input to the subsequent bbmerge process.     Fig. 3: How we get...

... was composed of many protein subunits, it was nonetheless a new subtype. Cas7-11 had combined all the... complex Class I systems that contain multiple subunits, but the more they worked with it, the more they...

...sequence results to full-length predicted sequences submitted by depositors. A sample will automatically pass...backbone sequences. Most theoretical sequences submitted to Addgene have minor differences due to in silico...10.1038/nbt1486  Slatko, B. E., Gardner, A. F., & Ausubel, F. M. (2018). Overview of Next‐Generation sequencing...