We narrowed to 451 results for: SUP
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TypeBlog Post...hands-on science lab kits and digital curricula to support them. Caitlin’s take on her job as an SME: “I write...a lot of interactives and videos, as well, to supplement the hands-on [aspect of the labs],” says Caitlin...
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Oh, The Places You Can Go: Careers in Science Communication - Product Development
TypeBlog Post...hands-on science lab kits and digital curricula to support them. Caitlin’s interview is broken into two parts...hands-on science lab kits and digital curricula that support these kits, is the perfect blend of these two passions... -
Tetbow: Bright Multicolor Labeling for Neuronal Tracing
TypeBlog Post...reduced expression level of XFPs, most likely by suppressing transcription. It is critical to express a minimal...Central PMCID: PMC3713494. Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal circuitry with an... -
5 Uncommon Ways to Gain Career Skills During an Uncommon Time
TypeBlog Post...mentoring support we need, and most of us could use more mentors to listen, guide and support us during... -
Three Tips for Preventing Viral Plasmid Recombination in Your Samples
TypeBlog Post...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...expected to form 3 bands: nicked, linear, and supercoiled. Here we find a smaller, recombined plasmid around... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog Post...sort of talent agency for scientists, where we support health science graduate students with innovative...fund that would apply a percentage of returns to support student projects, but it quickly became apparent... -
Mentoring for Scientists: I Have a Mentor, What Now?
TypeBlog Post...finding mentors and, perhaps, asked someone to support your career development as your mentor. How do ...your creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel... -
Improving Transduction Efficiency with Exosome AAVs
TypeBlog Post...spontaneously released from the producer cells into the supernatant during the production phase. However, this has...centrifugation steps to separate cell culture supernatant containing exo-AVVs from producer cells, and ... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog Post...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3. Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2. Improved trafficking and localization... -
Hot Plasmids - January 2023
TypeBlog Post...demands of advanced microscopy applications such as super resolution, electron, and expansion microscopy. ...with similar capabilities. If you’re looking for a super stable FP for your next microscopy experiment, consider... -
Management for Scientists: Delegating is Key
TypeBlog Post...ultimately responsible for outcomes. Be ready to support your team and take responsibility if things go ...want to know about right away? Provide adequate support. The manager must be available to answer questions... -
Choosing Your Fluorescent Proteins for Multi-Color Imaging
TypeBlog Post...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...an even newer green fluorescent protein that is supposed to be extremely bright. In S. cerevisiae, we've... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog Post...High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. ...several conformations the most abundant being: supercoiled, relaxed and nicked. If your digest lanes look... -
Advice for Scientists Starting a Lab
TypeBlog Post...go about starting a lab? Obviously, funding and support are crucial parameters, and familiarity with equipment...seek out advice from seasoned PIs who can offer support. “It is an exciting and emotional time!” George... -
22 Hot Plasmid Technologies from 2014
TypeBlog Post...genetic electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared...yeast display. APEX2 has been shown to exhibit superior performance over APEX for both EM imaging and ...protein expression Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck... -
A Deep Dive into BioBuilder
TypeBlog Post...with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group meetings and...the BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist ... -
Communicating Your Science With Help From ComSciCon
TypeBlog Post...education and policy and to draw attention and support to work in their field. It can be hugely beneficial...ComSciCon, our primary mission is to strengthen and support the network of graduate students who are passionate... -
Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME
TypeBlog Post...neurotoxin halves to enable VAMP2 cleavage, and suppresses neuronal transmission within minutes. Constructs...depositors demonstrated the utility of light-mediated suppression of synaptic transmission in brain slices from... -
Clear and Accessible Writing
TypeBlog Post...between layers are muddled due to the passage of supernatant through each layer. The right panel illustrates...before and after ultracentrifugation. The viral supernatant migrates through increasingly dense layers until... -
How Addgene Creates Content
TypeBlog Post...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...