...do. From all of us to all of you. Thanks for supporting nonprofit, open science and Addgene in our mission...Collaboration with Fellow Nonprofit Seeding Labs Addgene Supports Rare Disease Research Listen to Our Interview ...

...fluorescent signals. Named for the "stellar explosion SUperNova," SunTag can help you turn up the brightness in...GFP aggregation, which they reduced by using superfolder GFP (sfGFP) with the small solubility tag GB1...

...reduced expression level of XFPs, most likely by suppressing transcription. It is critical to express a minimal...Central PMCID: PMC3713494.  Ke, Meng-Tsen, et al. "Super-resolution mapping of neuronal circuitry with an...

...Working on the scientific support team at Addgene, I get to see everything that can go wrong with a plasmid...expected to form 3 bands: nicked, linear, and supercoiled. Here we find a smaller, recombined plasmid around...

...mentoring support we need, and most of us could use more mentors to listen, guide and support us during...

...sort of talent agency for scientists, where we support health science graduate students with innovative...fund that would apply a percentage of returns to support student projects, but it quickly became apparent...

...finding mentors and, perhaps, asked someone to support your career development as your mentor. How do ...your creative thinking Enhance leadership skills Support inclusion, diversity, and open communication Feel...

...spontaneously released from the producer cells into the supernatant during the production phase. However, this has...centrifugation steps to separate cell culture supernatant containing exo-AVVs from producer cells, and ...

...demands of advanced microscopy applications such as super resolution, electron, and expansion microscopy. ...with similar capabilities. If you’re looking for a super stable FP for your next microscopy experiment, consider...

...variants. Image adapted from Aggarwal et al…, Supplementary Figure 3.   Figure 3: pH titration...variants. Image adapted from Aggarwal et al…, Supplementary Figure 2.   Improved trafficking and localization...

...ultimately responsible for outcomes. Be ready to support your team and take responsibility if things go ...want to know about right away? Provide adequate support. The manager must be available to answer questions...

...biliverdin as a cofactor and so you may need to supplement your cells with biliverdin for maximal brightness...an even newer green fluorescent protein that is supposed to be extremely bright. In S. cerevisiae, we've...

...High Fidelity versions of the endonucleases and/or supply custom buffers as means to avoid this issue. ...several conformations the most abundant being: supercoiled, relaxed and nicked. If your digest lanes look...

...go about starting a lab? Obviously, funding and support are crucial parameters, and familiarity with equipment...seek out advice from seasoned PIs who can offer support. “It is an exciting and emotional time!” George...

...genetic electron microscopy (EM) tag providing superior subcellular localization of tagged proteins compared...yeast display. APEX2 has been shown to exhibit superior performance over APEX for both EM imaging and ...protein expression Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck...

...with a kickoff meeting in October, BioBuilderClub supports teams through monthly online group meetings and...the BioBuilder textbook, a laboratory kit, and support during the academic year from remote scientist ...

...neurotoxin halves to enable VAMP2 cleavage, and suppresses neuronal transmission within minutes. Constructs...depositors demonstrated the utility of light-mediated suppression of synaptic transmission in brain slices from...

...conversations, conferences, and questions our scientific support staff receives – in fact, the blog originally started... post (email [email protected].) We are here to support and advance the scientific community, and we can...

...between layers are muddled due to the passage of supernatant through each layer. The right panel illustrates...before and after ultracentrifugation. The viral supernatant migrates through increasingly dense layers until...

...education and policy and to draw attention and support to work in their field. It can be hugely beneficial...ComSciCon, our primary mission is to strengthen and support the network of graduate students who are passionate...